Avicenna Research Institute

Tehran, Iran

Avicenna Research Institute ) – affiliated to ACECR – was established in Tehran in order to achieve latest medical technologies through conducting clinical and laboratory research projects in 1998. The institute consists of three research centers including Reproductive Biotechnology Research Center , Monoclonal Antibody Research Center and Nanobiotechnology Research Center . ARI also includes Avicenna Biotechnology Incubator and Avicenna Infertility Clinic .Named after Ibn Sina, the research institute was found in 1998 in Shahid Beheshti University in Tehran.Reproductive Biotechnology Research Center includes Embryology and Andrology, Reproductive Genetics and Biotechnology, Reproductive Immunology, Reproductive Infections and Bio law & Ethics departments.For the purpose of laying a ground to provide therapeutic services and applied research findings in the field of infertility treatment, Avicenna Infertility Clinic was founded. Five specialized clinics including Infertility Treatment, Recurrent Abortion, Perinatology, Sexual Health and Replacement Therapies organize AIC.Monoclonal Antibody Research Center established in 2001 consists of Hybridoma, Immunochemistry and Antibody-Antigen Engineering Departments.Following introduction of nanotechnology and nanobiotechnology as one of the main priorities of the country, Nanobiotechnology Research Center was founded in 2005. Three departments including Nanotechnology, Recombinant Technology and Medical Diagnostic Products form NBRC.Interaction between specialists of various professional fields such as biology, biotechnology and clinical and molecular medicine in 11 research departments provide the ground for educational and scientific activities.Considering modern equipment and enjoying specialized team in Avicenna Research Institute, Medical Biotechnology Incubator initiated its activity under technical, economical and scientific supports of ARI, in 2010.The main activities of this center are summarized in the fields of reproductive biotechnology, monoclonal antibodies, nanobiotechnology, etc. All the established sections in this center have the possibility to benefit from the facilities and services provided by Reproductive Biotechnology Research Center, Monoclonal Antibody Research Center, Nanobiotechnology Research Center and Avicenna Infertility Clinic.Medical Biotechnology Incubator will foster new professions and encourage entrepreneurs working within the newly founded units in the above fields.The most prominent missions of ARI: Conducting basic, applied and therapeutic research projects in the field of reproduction and cancer treatment Identifying and fulfilling requirements related to biotechnologies in diagnostic and therapeutic areas Attracting M.Sc and Ph.D students to conduct thesis Arranging postgraduate courses on modern technologies in collaboration with different universities Giving information, publishing books and journals as well as holding national, regional and international conferences Wikipedia.

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Hosseinzadehdehkordi M.,Tarbiat Modares University | Adelinik A.,Avicenna Research Institute | Tashakor A.,Islamic Azad University at Varamin
European Journal of Pharmacology | Year: 2015

Exposure to arsenic is one of the major causes of lung cancer due to production of Reactive Oxygen Species (ROS). Herbal medicine is a new approach used for prevention or treatment of cancers. Among various herbal compounds, a lot of attention has been paid to curcumin, as antioxidant, anti-proliferative, anti-carcinogenic and anti-tumor and pro-apoptotic properties of curcumin have been well studied. In the present study, we investigated the effects of curcumin on lung cancer cell lines and arsenic-treated lung cancer cell lines, originated from different stages of lung cancer development. Here, we measured ROS generation and caspase 3/7 activity for both curcumin-treated cell lines and those co-treated with arsenic and curcumin. Then, we studied lipid peroxidation, intracellular ATP content, and cytochrome c release to further investigate how ROS generation and curcumin exert synergistic effects and direct cells toward apoptosis. According to our data, curcumin has a dual effect on ROS generation which is dependent on specific concentration as a threshold and seems to induce apoptosis by two different mechanisms. Moreover, for the first time we report that curcumin delays the drop in ATP levels in these cell lines and hence provides required energy for apoptosis process. Furthermore, western blot analysis reveals that release of cytochrome c is highest when ATP begins to drop in the presence of curcumin. To sum it up, it seems that curcumin is strong candidate for prevention or treatment of lung cancer, especially at stage 2. © 2015 Elsevier B.V. All rights reserved.

Nazari M.,Shahid Beheshti University of Medical Sciences | Ghorbani A.,Shahid Beheshti University of Medical Sciences | Hekmat-Doost A.,Shahid Beheshti University of Medical Sciences | Jeddi-Tehrani M.,Avicenna Research Institute | Zand H.,Shahid Beheshti University of Medical Sciences
European Journal of Pharmacology | Year: 2011

Hesperidin, a flavonoid present in fruits and vegetables, possesses anti-inflammatory and chemopreventive effects. Some evidence implies that hesperidin may recruit Peroxisome Proliferator-Activated Receptor-gamma (PPARγ) to exert biological action. It has been shown also that inactivation of Nuclear Factor-kappaB is another mechanism of flavonoids' action. Therefore, we wished to investigate the role of these transcription factors in apoptosis induced by hesperidin in Ramos Burkitt's lymphoma cells. We found that incubation of Ramos cells with hesperidin abrogates constitutive and doxorubicin-induced NF-κB activation through inhibition of phosphorylation of its inhibitory subunits (IκB). Moreover, our results showed that incubation of Ramos cells with hesperidin resulted in inhibition of proliferation and apoptosis in a PPARγ-independent way. Our results demonstrated that hesperidin inhibits proliferation of Ramos cells and sensitizes them to doxorubicin-induced apoptosis through inhibition of both constitutive and doxorubicin-mediated NF-κB activation in a PPARγ-independent manner. © 2010 Elsevier B.V. All rights reserved.

Habibi Z.,Shahid Beheshti University | Mohammadi M.,Iran National Institute of Genetic Engineering and Biotechnology | Yousefi M.,Avicenna Research Institute
Process Biochemistry | Year: 2013

This research describes the immobilization of Rhizomucor miehei lipase (RML) and chemically aminated RML (NH2-RML) on different supports including octyl-sepharose (octyl-RML), activated sepharose with cyanogen bromide (CNBr-RML and CNBr-NH2-RML), glyoxyl sepharose (Gx-RML and Gx-NH2-RML) and glyoxyl sepharose dithiothreitol (Gx-DTT-RML and Gx-DTT-NH2-RML). The highest immobilization yield was achieved for octyl-RML (>98%) followed by CNBr-RML (88%). Octyl-RML had the most specific activity (13.6) among all derivatives. The other preparations had moderate activities likely because of chemical reaction during covalent attachment of the enzyme. The catalytic behavior of lipase immobilized in hydrolysis reactions was investigated using methyl, ethyl, propyl, butyl and isobutyl-ibuprofen esters and the influence of the alkyl chain and the alcoholic residue of the ester were studied. Butyl ester was the most interesting ester for carrying out hydrolysis. The highest enantioselectivity of enzyme (E = 8.8) was obtained with isooctane/sodium phosphate buffer pH 7.0 at temperature of 40 °C. Increasing temperature from 40 to 50 °C caused decreasing in enantioselectivities and conversions. Also esterification of ibuprofen was carried out in solvent systems containing isooctane and two ionic liquids (ILs); [BMIM][PF6] and [BMIM][BF4]. Poor conversions and enantioselectivities were observed during esterification in all solvents. © 2013 Elsevier Ltd.

Oryan A.,Shiraz University | Alidadi S.,Shiraz University | Moshiri A.,Shiraz University | Moshiri A.,Avicenna Research Institute | And 2 more authors.
Journal of Orthopaedic Surgery and Research | Year: 2014

This review analyzes the literature of bone grafts and introduces tissue engineering as a strategy in this field of orthopedic surgery. We evaluated articles concerning bone grafts; analyzed characteristics, advantages, and limitations of the grafts; and provided explanations about bone-tissue engineering technologies. Many bone grafting materials are available to enhance bone healing and regeneration, from bone autografts to graft substitutes; they can be used alone or in combination. Autografts are the gold standard for this purpose, since they provide osteogenic cells, osteoinductive growth factors, and an osteoconductive scaffold, all essential for new bone growth. Autografts carry the limitations of morbidity at the harvesting site and limited availability. Allografts and xenografts carry the risk of disease transmission and rejection. Tissue engineering is a new and developing option that had been introduced to reduce limitations of bone grafts and improve the healing processes of the bone fractures and defects. The combined use of scaffolds, healing promoting factors, together with gene therapy, and, more recently, three-dimensional printing of tissue-engineered constructs may open new insights in the near future. © 2014 Oryan et al.; licensee BioMed Central Ltd.

Yousefi M.,Avicenna Research Institute | Mohammadi M.,Iran National Institute of Genetic Engineering and Biotechnology | Habibi Z.,Shahid Beheshti University
Journal of Molecular Catalysis B: Enzymatic | Year: 2014

Here we report the stereoselective hydrolysis of racemic esters catalyzed by Candida rugosa lipase (CRL) and Rhizopus oryzae lipase (ROL) immobilized on octyl-sepharose via physical adsorption. Hydrophobic immobilization caused to almost six fold hyperactivation with 229.2 and 81.3 U/mg enzyme for immobilized CRL and ROL, respectively (13.2 and 48.75 U/mg for corresponding free enzyme). Based on the preliminary results, CRL was chosen for further investigation. The performance and yield of the reaction were evaluated as a function of the critical reaction parameters such as temperature, enzyme to substrate ratio and organic co-solvent. An increase in the temperature resulted to decrease in enantioselectivity of hydrolysis reaction. The hydrolysis reactions were carried out in presence of two organic solvents; n-hexane and isooctane. Generally n-hexane was a better co-solvent compared to isooctane. High enantioselective hydrolysis of the racemic esters (yielding S(+) ibuprofen; ee ≤ 95%) can be achieved using the immobilized CRL. Among various esters the kinetic resolution of ibuprofen butyl ester yielded the best results (E value 70 and 74; conversion 14.6 and 8.9 in n-hexane and isooctane, respectively). The immobilized derivatives were re-used in four cycles and showed little decrease in enantiomeric excess of (S)-ibuprofen. 96.7 eep and conversion 14.6 in first cycle reached to 90.5 eep and conversion 11.3 in the forth cycle. © 2014 Elsevier B.V.

Bazgir A.,Shahid Beheshti University | Hosseini G.,Shahid Beheshti University | Ghahremanzadeh R.,Avicenna Research Institute
ACS Combinatorial Science | Year: 2013

A green reaction of isatins, active cyanomethanes, and cyclic 1,3-dicarbonyl derivatives for the efficient and simple one-pot three-component synthesis of spirooxindole fused heterocycles in refluxing water by use of magnetically recoverable and reusable catalyst is reported. The features of this procedure are, the use of magnetically recoverable and reusable catalyst, mild reaction conditions, high to excellent product yields, operational simplicity, and easy workup procedures. Most importantly of all, easy magnetic separation of the catalyst eliminates the requirement of catalyst filtration after completion of the reaction. Furthermore, the catalyst remained highly active even after 5 repeated uses. © 2013 American Chemical Society.

Ghahremanzadeh R.,Avicenna Research Institute | Amanpour T.,Shahid Beheshti University | Bazgir A.,Shahid Beheshti University
Tetrahedron Letters | Year: 2010

An efficient and simple method for the synthesis of new benzopyranopyrimidines via a pseudo four-component reaction of salicylic aldehydes, malononitrile and various amines in the presence of a catalytic amount of LiClO4 is reported. The advantages of this procedure are mild reaction conditions, high yields of products and operational simplicity. © 2010 Elsevier Ltd. All rights reserved.

Naeimi H.,University of Kashan | Rashid Z.,University of Kashan | Zarnani A.H.,Avicenna Research Institute | Ghahremanzadeh R.,Avicenna Research Institute
New Journal of Chemistry | Year: 2014

A one-pot and three-component reaction for the efficient, green and economical synthesis of novel spiro-furo-pyrido-pyrimidine-indolines using 2,6-diaminopyrimidine-4(3H)-one or uraciles, isatins and tetronic acid or anilinolactones in the presence of manganese ferrite nanoparticles as a magnetic catalyst in water is described. The present synthesis shows attractive characteristics such as; the use of magnetically recoverable and reusable catalyst, convenient one-pot operation, short reaction periods, good to high yields and the use of water as a green reaction medium and is considered to be relatively environmentally benign. This journal is © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2014.

Fazeli B.,Avicenna Research Institute | Rezaee S.A.,Mashhad University of Medical Sciences
Vascular | Year: 2011

A century has passed since thromboangiitis obliterans (TAO), or Buerger's disease, was first described, but the etiology remains unclear. It is still uncertain as to whether thrombosis or vascular inflammation is the first event. TAO is an episodic inflammatory and thrombotic-occlusive vascular disease of unknown origin. The involvement of the distal vessels and nerves within the neuro-vascular bundles occurs almost always in legs and occasionally in arms. The cumulative data demonstrate that at the cellular and molecular levels, at least four main components of inflammatory reactions, including endothelial cells, platelets, leukocytes and sensory neurons, might be involved in TAO pathogenesis. The interactions among these cells in an altered microenvironment of small- and medium-sized vessels may also orchestrate the onset of TAO events. In this review, the factors that may promote thrombosis and angiitis are reconsidered at three levels: (1) host characteristics such as male gender and genetic background; (2) probable triggers including cigarette smoking and infectious agents; and (3) environmental factors such as chronic anxiety and mental stress as a consequence of low socioeconomic status. At each level, the interactions among vascular endothelium, platelets, leukocytes and sensory neurons are discussed. © 2011 Royal Society of Medicine Press. All rights reserved.

Avicenna Research Institute | Date: 2014-01-30

An in vitro method for assessing the risk that a subject suffers from a cancer, comprising measuring the expression level of Proline/arginine-rich end leucine repeat protein (PRELP) in cells from said subject, wherein an increased expression level of PRELP, as compared to healthy donors, indicates an increased probability of said subject suffering from cancer. The disclosure further relates to antibodies specific for PRELP and their use in diagnosis and therapy as well as a method for indicating a cell as a cancer cell.

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