Pastushkova L.K.,Aviakosmicheskaya I Ekologlcheskaya Meditsina |
Pakharukova N.A.,Aviakosmicheskaya I Ekologlcheskaya Meditsina |
Novoselova N.M.,Aviakosmicheskaya I Ekologlcheskaya Meditsina |
Dobrokhotov I.V.,Aviakosmicheskaya I Ekologlcheskaya Meditsina |
And 3 more authors.
Aviakosmicheskaya i Ekologicheskaya Meditsina | Year: 2012
Changes in proteome of urine and blood serum obtained from 14 healthy humans (age 21-29 yrs) medically certified for an experiment with dry immersion were analyzed. Urine and serum samples were prefractionated and enriched with magnetic particles MBWCX and MB-HIC, respectively, on robot ClinProt (Bruker Daltonics) for direct mass-spectrometry profiling by MALDI-TOF. As a result, 143 protein peaks on the average were identified in urine samples. It was shown that a high variation coefficient in 23.7% of protein peaks, i.e. double technical, points to the most plastic fraction of the urine proteome. In blood serum, 175 peaks were identified in a sample on the average. Comparison of baseline and immersion mass-spectra of the blood proteome revealed significant differences. Increased peak areas of several protein fragments - C3 and C4 fragments of complement system, highmolecular kininogen and fibrinogen - can be ascribed to human body adaptation to the experimental conditions.