Quintero-Hernandez V.,Avenida University |
Ortiz E.,Avenida University |
Rendon-Anaya M.,Avenida University |
Schwartz E.F.,University of Brasilia |
And 3 more authors.
Toxicon | Year: 2011
This communication reviews most of the important findings related to venom components isolated from scorpions and spiders, mainly by means of gene cloning and expression. Rather than revising results obtained by classical biochemical studies that report structure and function of venom components, here the emphasis is placed on cloning and identification of genes present in the venomous glands of these arachnids. Aspects related to cDNA library construction, specific or random ESTs cloning, transcriptome analysis, high-throughput screening, heterologous expression and folding are briefly discussed, showing some numbers of species and components already identified, but also shortly mentioning limitations and perspectives of research for the future in this field. © 2011 Elsevier Ltd.
Garcia Y.-M.,Avenida University |
Munoz M.,Avenida University |
Mejia J.,Avenida University |
Gasca-Hurtado G.-P.,University of Medellín
Iberian Conference on Information Systems and Technologies, CISTI | Year: 2017
Software production is an increasingly important economic activity; in Latin America, this sector is made up of 99% of SMEs. This highlights the importance of the implementation of Software Engineering best practices in SMEs to improve their productivity. The goal of this article is to present an analysis of project management techniques and tools. The analysis focuses on project planning, project monitoring and control, as support of SMEs in the selection of those techniques and tools appropriate to their environment, so that, the use of and implementation of best practices is promoted. © 2017 AISTI.
Wieneke L.,Center Virtuel Of La Connaissance Sur Leurope |
During M.,Center Virtuel Of La Connaissance Sur Leurope |
Silaume G.,Center Virtuel Of La Connaissance Sur Leurope |
Lallemand C.,CRP Henri Tudor |
And 11 more authors.
Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) | Year: 2014
The breadth and scale of multimedia archives provides a tremendous potential for historical research that hasn't been fully tapped up to know. In this paper we want to discuss the approach taken by the History of Europe application, a demonstrator for the integration of human and machine computation that combines the power of face recognition technology with two distinctively different crowd-sourcing approaches to compute co-occurrences of persons in historical image sets. These co-occurrences are turned into a social graph that connects persons with each other and positions them, through information about the date and location of recording, in time and space. The resulting visualization of the graph as well as analytical tools can help historians to find new impulses for research and to un-earth previously unknown relationships. As such the integration of human expertise and machine computation enables a new class of applications for the exploration of multimedia archives with significant potential for the digital humanities. © 2014 Springer-Verlag.
Gao B.,CAS Institute of Zoology |
Xu J.,Renmin University of China |
del Carmen Rodriguez M.,Avenida University |
Lanz-Mendoza H.,Avenida University |
And 3 more authors.
Biochimie | Year: 2010
Plasmodium falciparum is a pathogen of human malaria which causes millions of deaths per year due to the ever-increasing drug resistance by the parasite, and thus novel antimalarial agents are urgently needed. In this work, we report two cDNA clones from the scorpion Mesobuthus eupeus venom gland, which encode peptides inhibiting the development of Plasmodium berghei, killing intraerythrocytic P. falciparum, and toxic to the Drosophila S2 cell at micromolar concentrations. One peptide of 24 amino acids (named meucin-24) shares high sequence identity to the amino-terminus of a family of scorpion venom long-chain K+ channel toxins (LcKTx) and two frog antimicrobial peptides (magainin1 and 2). Sequencing genomic DNA of meucin-24 identified this short peptide as a product of a putative guanine-to-adenine RNA editing from a M. eupeus LcKTx transcript. Another peptide, named meucin-25, contains 25 residues and does not share sequence similarity with any known peptides. Circular dichroism analysis of chemically synthesized peptides demonstrates that meucin-24 presents an essential random coil conformation in water, but its α-helical content largely increases in the presence of 50% trifluoroethanol, a membrane-mimicking environment. This finding was further verified by its NMR structure that showed an α-helical amphipathic architecture in the region of residues 4-20. CD results indicate that meucin-25 mainly adopts a β-sheet structure in water but TFE promotes its α-helical formation, suggesting its conformational flexibility. Killing of intraerythrocytic P. falciparum without harming mammalian cells (erythrocytes and GC-2 cell) make them attractive candidates for antimalarial drug design. © 2010 Elsevier Masson SAS. All rights reserved.
Zhu S.,CAS Institute of Zoology |
Gao B.,CAS Institute of Zoology |
Aumelas A.,Montpellier University |
del Carmen Rodriguez M.,Avenida University |
And 6 more authors.
Biochimica et Biophysica Acta - Proteins and Proteomics | Year: 2010
Recent studies have demonstrated that scorpion venom contains unique two-domain peptides with the peculiarity of possessing different functions, i.e. neurotoxic and cytolytic activities. Here we report systematic characterization of a new two-domain peptide (named MeuTXKβ1) belonging to the TsTXKβ molecular subfamily from the scorpion Mesobuthus eupeus by molecular cloning, biochemical purification, recombinant expression, functional assays, CD and NMR studies. Its full-length bioactive form as well as 1-21 and 22-72 fragments (named N(1-21) and C(22-72), respectively) was produced in Escherichia coli by an on-column refolding approach. Recombinant peptide (rMeuTXKβ1) exhibited a low affinity for K+ channels and cytolytic effects against bacteria and several eukaryotic cells. N(1-21) was found to preserve anti-Plasmodium activity in contrast to haemolytic activity, whereas C(22-72) retains these two activities. Circular dichroism analysis demonstrates that rMeuTXKβ1 presents a typical scorpion toxin scaffold in water and its α-helical content largely increases in a membrane-mimicking environment, consistent with the NMR structure of N(1-21) and an ab initio structure model of MeuTXKβ1 predicted using I-TASSER algorithm. Our structural and functional data clearly indicate an evolutionary link between TsTXKβ-related peptides and antiparasitic scorpines which both comprise the βSPN (β-KTxs and scorpines) family. © 2009 Elsevier B.V. All rights reserved.
Crescenti A.,Avenida University |
Puiggros F.,Avenida University |
Colome A.,Laboratorios Deiters S.L. Badalona |
Poch J.A.,Laboratorios Deiters S.L. Badalona |
And 5 more authors.
Archivos Espanoles de Urologia | Year: 2015
OBJECTIVE: To determine the effect of a botanical formulation of Herniaria glabra, Agropyron repens, Equisetum arvense, and Sambucus nigra as a preventive agent in an experimentally induced nefrolithiasis model in rats. METHODS: Six groups of six Wistar male rats each were induced for nefrolithiasis by treatment with 0.75% ethylene glycol (EG) and 1% ammonium chloride for three days and then EG only for 15 days. One group was treated with placebo (control group) and the other groups (treated groups) were treated with 30 mg/Kg, 60 mg/Kg, 125 mg/Kg, 250 mg/Kg and 500 mg/Kg of the plant extract formulation (PEF). 24-h urine and water samples were collected one day before EG administration and at 7, 13 and 18 days to determine diuresis, crystalluria and urine biochemistry. The kidneys were removed for histological analysis. The phytochemical characterization of PEF and each of its component plant extracts was performed using gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry. RESULTS: Animals treated with 125 mg/Kg of the PEF had statistically significantly lower calcium oxalate crystals deposits content compared to the control group. All PEF doses statistically significantly decreased the number of microcalcifications compared to the control group. Furthermore, the number of kidneys affected by subcapsular fibrosis was statistically significantly higher in control group than in treated groups with the PEF. The diuresis of the 125 mg/Kg and 500 mg/Kg PEF-treated groups was statistically significantly higher than that of the control group. A phytochemical analysis demonstrated the presence of flavonoids, dicarboxylic acids and saponins. CONCLUSION: Treatment with PEF prevents deposits of calcium oxalate crystals formation and of microcalcifications in the kidney, and reduces the risk of fibrosis subcapsular. 125 mg/Kg of PEF is the dose that has a greater effect on the studied parameters.
Arellano-Vazquez M.,National Autonomous University of Mexico |
Benitez-Perez H.,National Autonomous University of Mexico |
Ortega-Arjona J.,Avenida University
International Journal of Distributed Sensor Networks | Year: 2015
This paper introduces a consensus routing algorithm based on an availability function. Such a function obtains a consensus among a group of nodes by evaluating the idle time in the scheduler of each node, along with the general conditions of the network and determining the next step of the route. In this regard, the algorithm proposed here makes it possible to avoid flooding while reducing bandwidth use and keeping changes of the network only at a local scale. © 2015 Magali Arellano-Vázquez et al.
Cervantes-Garcia E.,Avenida University |
Cervantes-Garcia E.,Laboratorio Of Mucosas |
Garcia-Gonzalez R.,Laboratorio Of Mucosas |
Resendiz-Albor A.,Laboratorio Of Mucosas |
Salazar-Schettino P.M.,Avenida University
International Journal of Lower Extremity Wounds | Year: 2015
Infected diabetic foot is the most common reason for hospitalization and complications in patients with type 2 diabetes mellitus (DM2). Methicillin-resistant Staphylococcus aureus (MRSA) is frequently isolated from such lesions, and its presence is growing, seriously deteriorating the infected patient's quality of life. The aim of this study was to assess the prevalence of MRSA as well as other microbiota in 100 patients diagnosed with (DM2) and with infected foot ulcers at the Hospital General de Mexico. The main results obtained show a prevalence of Staphylococcus aureus (42%), followed by Escherichia coli (36%) and, in lower percentages, other bacteria. MRSA was predominant (34%), and we conclude that the use of cefoxitin instead of oxacillin as the first-choice antibiotic has an advantage because it is a better inducer of methicillin-resistance expression. © The Author(s) 2015.
Becerril-Alquicira A.,Avenida University |
Ortiz-Posadas M.R.,Metropolitan Autonomous University
2010 Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC'10 | Year: 2010
The purpose of this study was to make a proposal to improve the management process of the health technology within the Health Service at Morelos State, México, using the five stages of Six Sigma methodology: Definition, Measurement, Analysis, Improvement and Control. Up to date these five steps have been executed resulting in a set of proposals to improve the current health technology management process. This will allow the establishment of a medical equipment control program that impacts the three levels of Health Care Service in Morelos State in Mexico. © 2010 IEEE.
PubMed | Avenida University
Type: Journal Article | Journal: Toxicon : official journal of the International Society on Toxinology | Year: 2011
This communication reviews most of the important findings related to venom components isolated from scorpions and spiders, mainly by means of gene cloning and expression. Rather than revising results obtained by classical biochemical studies that report structure and function of venom components, here the emphasis is placed on cloning and identification of genes present in the venomous glands of these arachnids. Aspects related to cDNA library construction, specific or random ESTs cloning, transcriptome analysis, high-throughput screening, heterologous expression and folding are briefly discussed, showing some numbers of species and components already identified, but also shortly mentioning limitations and perspectives of research for the future in this field.