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Cuernavaca, Mexico

Oviedo M.J.,National Autonomous University of Mexico | Contreras O.E.,National Autonomous University of Mexico | Rosenstein Y.,Avenida University 2001 | Vazquez-Duhalt R.,National Autonomous University of Mexico | And 3 more authors.
Journal of Nanomaterials | Year: 2016

Bismuth germanate (Bie BGO) has been the focus of several studies due to its scintillation properties. It has been employed as detector in scientific research and medicine, and herein we studied its possible biomedical applications. The photoluminescence properties of the uncoated and protein-coated nanoparticles were analyzed in different body fluids, at different pH. The nanoparticles yielded blueish-white luminescence with a maximum emission peak at 485 nm corresponding to the 3P→ 1Selectron transition of Bi3+. They showed luminescence properties at different pH values and in human fluids, such as urine and blood serum. Finally, the BGO nanoparticles were functionalized with the anti-HLA I W6/32 monoclonal antibody and the capacity of the antibody-loaded nanoparticles to recognize the cognate antigen (HLA I) of the W6/32 mAb was assessed on the human promyelocytic leukemia cell line THP-1. The possibility of functionalizing BGO nanoparticles with W6/32 antibodies and their specificity to identify THP-1 cells make them promising candidates for biomedical applications as biolabels. © 2016 M. J. Oviedo et al. Source

Deuis J.R.,University of Queensland | Zimmermann K.,Friedrich - Alexander - University, Erlangen - Nuremberg | Romanovsky A.A.,systemIC | Possani L.D.,Avenida University 2001 | And 3 more authors.
Pain | Year: 2013

Cold allodynia, pain in response to cooling, occurs during or within hours of oxaliplatin infusion and is thought to arise from a direct effect of oxaliplatin on peripheral sensory neurons. To characterize the pathophysiological mechanisms underlying acute oxaliplatin-induced cold allodynia, we established a new intraplantar oxaliplatin mouse model that rapidly developed long-lasting cold allodynia mediated entirely through tetrodotoxin-sensitive Nav pathways. Using selective inhibitors and knockout animals, we found that Nav1.6 was the key isoform involved, while thermosensitive transient receptor potential channels were not involved. Consistent with a crucial role for delayed-rectifier potassium channels in excitability in response to cold, intraplantar administration of the K +-channel blocker 4-aminopyridine mimicked oxaliplatin-induced cold allodynia and was also inhibited by Nav1.6 blockers. Intraplantar injection of the Nav1.6 activator Cn2 elicited spontaneous pain, mechanical allodynia, and enhanced 4-aminopyridine-induced cold allodynia. These findings provide behavioural evidence for a crucial role of Nav1.6 in multiple peripheral pain pathways including cold allodynia. © 2013 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved. Source

Singla T.,Indian Institute of Technology Bombay | Montoya F.,Avenida University 2001 | Rivera M.,Autonomous University of Mexico State | Tajima S.,Saitama University | And 2 more authors.
Chaos | Year: 2016

We report synchronization of Mercury Beating Heart (MBH) oscillators using the environmental coupling mechanism. This mechanism involves interaction of the oscillators with a common medium/environment such that the oscillators do not interact among themselves. In the present work, we chose a modified MBH system as the common environment. In the absence of coupling, this modified system does not exhibit self sustained oscillations. It was observed that, as a result of the coupling of the MBH oscillators with this common environment, the electrical and the mechanical activities of both the oscillators synchronized simultaneously. Experimental results indicate the emergence of both lag and the complete synchronization in the MBH oscillators. Simulations of the phase oscillators were carried out in order to better understand the experimental observations. © 2016 Author(s). Source

Bush S.P.,East Carolina University | Ruha A.-M.,Banner Good Samaritan Medical Center | Seifert S.A.,University of New Mexico | Morgan D.L.,Scott and White Memorial Hospital | And 16 more authors.
Clinical Toxicology | Year: 2015

Background. Crotalidae Polyvalent Immune Fab (Ovine) has been the only antivenom commercially available in the US since 2007 for treatment of Crotalinae envenomation. Late coagulopathy can occur or recur after clearance of Fab antivenom, often after hospital discharge, lasting in some cases more than 2 weeks. There have been serious, even fatal, bleeding complications associated with recurrence phenomena. Frequent follow-up is required, and additional intervention or hospitalization is often necessary. F(ab')2 immunoglobulin derivatives have longer plasma half life than do Fab. We hypothesized that F(ab')2 antivenom would be superior to Fab in the prevention of late coagulopathy following treatment of patients with Crotalinae envenomation. Methods. We conducted a prospective, double-blind, randomized clinical trial, comparing late coagulopathy in snakebitten patients treated with F(ab')2 with maintenance doses [F(ab')2/F(ab')2], or F(ab')2 with placebo maintenance doses [F(ab')2/placebo], versus Fab with maintenance doses [Fab/Fab]. The primary efficacy endpoint was coagulopathy (platelet count < 150 K/mm3, fibrinogen level < 150 mg/dL) between end of maintenance dosing and day 8. Results. 121 patients were randomized at 18 clinical sites and received at least one dose of study drug. 114 completed the study. Of these, 11/37 (29.7%) in the Fab/Fab cohort experienced late coagulopathy versus 4/39 (10.3%, p < 0.05) in the F(ab')2/F(ab')2 cohort and 2/38 (5.3%, p < 0.05) in the F(ab')2/placebo cohort. The lowest heterologous protein exposure was with F(ab')2/placebo. No serious adverse events were related to study drug. In each study arm, one patient experienced an acute serum reaction and one experienced serum sickness. Conclusions. In this study, management of coagulopathic Crotalinae envenomation with longer-half-life F(ab')2 antivenom, with or without maintenance dosing, reduced the risk of subacute coagulopathy and bleeding following treatment of envenomation. © 2014 Informa Healthcare USA, Inc. Source

Serrano-Posada H.,Avenida University 2001 | Valderrama B.,Avenida University 2001 | Stojanoff V.,Brookhaven National Laboratory | Rudio-Piera E.,Avenida University 2001
Acta Crystallographica Section F: Structural Biology and Crystallization Communications | Year: 2011

A thermostable multicopper oxidase from Thermus thermophilus HB27 (Tth-MCO) was successfully crystallized using the sitting-drop and hanging-drop vapour-diffusion methods. Crystallization conditions and preliminary X-ray diffraction data to 1.5 Å resolution obtained using synchrotron radiation at 100 K are reported. The crystals belonged to space group C2221, with unit-cell parameters a = 93.6, b = 110.3, c = 96.3 Å. A monomer in the asymmetric unit yielded a Matthews coefficient (V M) of 2.60 Å 3 Da -1 and a solvent content of 53%. An inactive enzyme form, apo-Tth-MCO, was also crystallized and diffraction data were collected to 1.7 Å resolution. In addition, a second inactive form of the enzyme, Hg-Tth-MCO, was obtained by soaking apo-Tth-MCO crystals with mercury(II) chloride and data were collected to a resolution of 1.7 Å. © 2011 International Union of Crystallography. All rights reserved. Source

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