Rodriguez-Cantu L.N.,Avenida Eugenio Garza Sada 2501 Sur |
Gutierrez-Uribe J.A.,Avenida Eugenio Garza Sada 2501 Sur |
Arriola-Vucovich J.,Avenida Eugenio Garza Sada 2501 Sur |
Diaz-De La Garza R.I.,Monterrey Institute of Technology |
And 2 more authors.
Journal of Agricultural and Food Chemistry | Year: 2011
This study investigated the effects of broccoli sprouts (BS) on sterol and lipid homeostasis in Syrian hamsters with dietary-induced hypercholesterolemia. Treatments included freeze-dried BS containing 2 or 20 μmol of glucoraphanine (BSX, BS10X), glucoraphanine-rich BS extract (GRE), sulforaphane-rich BS extract (SFE), and simvastatin. Each experimental diet was offered to eight animals (male and female) for 7 weeks. Hepatic cholesterol was reduced by BS10X and SFE treatments in all animals. This correlated with a down-regulation of gene expression of sterol regulatory element-binding proteins (SREBP-1 and -2) and fatty acid synthase (FAS) caused by GRE and SFE diets. BS10X caused changes in gene expression in a gender-specific manner; additionally, it increased coprostanol excretion in females. With the same concentration of glucoraphanin, consumption of broccoli sprouts (BS10X) had more marked effects on cholesterol homeostasis than GRE; this finding reinforces the importance of the matrix effects on the bioactivity of functional ingredients. © 2011 American Chemical Society.