de Fatima Rosas-Cardenas F.,National Polytechnic Institute of Mexico |
de Fatima Rosas-Cardenas F.,Research Center en Biotecnologia Aplicada del Instituto Polytechnic NacionalTlaxcala |
Escobar-Guzman R.,National Polytechnic Institute of Mexico |
Cruz-Hernandez A.,Autonomous University of QueretaroQueretaro |
And 2 more authors.
Frontiers in Plant Science | Year: 2015
microRNAs are a class of non-coding small RNAs (sRNAs) that are important regulators of gene expression at the post-transcriptional level by mRNA cleavage or translation inhibition. Another class of sRNAs are siRNAs, which also regulate gene expression but by causing DNA methylation. This epigenetic regulatory role has been observed for some miRNAs as well. The use of sRNAs allows the development of biotechnological applications in plants. To develop these types of applications, and to better understand the natural roles they play, it is important to be able to detect and to localize these sRNAs at the plant tissue level. Sometimes, in crop plants this can be challenging. Therefore, we developed a tissue printing hybridization protocol for easy and efficient detection of sRNAs and demonstrate this by the analysis of the spatio-temporal expression patterns of the miRNAs miR159 and miR164 in fruits of various crop plants. Moreover, we show the possibility to also detect the expression of miRNAs in fruit juice using a dot blot hybridization approach. © 2015 Rosas-Cárdenas, Escobar-Guzmán, Cruz-Hernández, Marsch-Martínez and de Folter.
Cu F.,Autonomous University of QueretaroQueretaro |
Rangel P.,Autonomous University of QueretaroQueretaro
Revista Chilena de Nutricion | Year: 2015
Introduction: At one year of age, factors that favor the develo- pment of obesity and other metabolic disorders in later stages of life are present. Objective: To identify perinatal, societal, and dietary risk factors for excess weight and obesity in 12 months infants. Subjects and methods: Case-control studies with infants under the care of Family Medicine Units. These cases studied were overweight or obese children, and control cases were children at a healthy weight. Each group included 152 infants, who were evaluated based on their responses to a questionnaire, their re- cords, and perinatal, societal, and dietary variables. Results: The variables with statistical significance were high pre-gestational weight, high gestational weight gain (OR 1.79-3.88), absence of breastfeeding and exclusive formula feeding (OR 2.02 and 1.75). Conclusion: In the presence of pre-pregnancy obesity and exclusive formula feeding, there is a 64.4% greater risk of becoming overweight or obese at 12 months old. © 2015, Sociedad Chilena de Nutricion Bromatologia y Toxilogica. All rights reserved.
De Moure-Flores F.,Autonomous University of QueretaroQueretaro |
Guillen-Cervantes A.,CINVESTAV |
Campos-Gonzalez E.,CINVESTAV |
Santoyo-Salazar J.,CINVESTAV |
And 7 more authors.
Materials Science in Semiconductor Processing | Year: 2015
Copper indium disulfide (CuInS2) thin films were prepared by chemical bath deposition in an acid medium on glass substrates. CuInS2 films were grown using CuSO4, InCl3 and C2H5NS as copper, indium and sulfur sources, respectively. The CuSO4 and C2H5NS concentrations remained constant, while the InCl3 concentration was varied from 0.002 M to 0.025 M. The structural analysis show that initially the films have a mixture of CuS and CuInS2 phases, when the indium nominal concentration increases the formation of CuInS2 ternary compound was promoted until the final formation of a CuInS2 film. The morphological study shows that the surface of CuInS2 films is constituted by nanotubes. The structural and compositional analysis show that for 0.025 M InCl3 concentration CuInS2 films were obtained. © 2015 Elsevier Ltd. All rights reserved.
Avila-Juarez L.,Autonomous University of QueretaroQueretaro |
Rodriguez Gonzalez A.,Autonomous University of QueretaroQueretaro |
Rodriguez Pina N.,Autonomous University of QueretaroQueretaro |
Guevara Gonzalez R.G.,Autonomous University of QueretaroQueretaro |
And 3 more authors.
Journal of Soil Science and Plant Nutrition | Year: 2015
Due to low yields, organic agriculture cannot satisfy the global demand for food, although it can provide products of higher nutraceutical quality. The objective of this research was to incorporate vermicompost leachates (VCLs) into an irrigation system during tomato (Solanum lycopersicum cv. Rafaello) cultivation to evaluate their effects on the lycopene, β-carotene, and phenolic content of tomatoes and on the physical and chemical soil variables. To evaluate the effects of VCLs, three types of substrates were used to create VCLs: mushroom waste (MSHW), leaf-cutting ant waste (LCAW), and cow compost (CC). A total of 0.1 L of leachate per plant was added as a supplement to a nutrient solution (NS) and applied once weekly, twice weekly, or every fifteen days to three different treatments for each leachate. All VCLs had a positive effect on the production of lycopene; the best results were obtained by the application of the MSHW (78 mg kg-1 fresh weight). The VCL decreased the presence of ions phytotoxic to plants by 99% and improved the soil structure by increasing the amount of organic matter and the hydraulic conductivity. However, the VCL had no effect on the physiological variables. The results support the use of leachate from CC via fertigation twice a week at a dose of 0.1 L plant -1 because it increases lycopene content by 67%. © 2015, Sociedad Chilena de la Ciencia del Suelo. All rights reserved.