The Autonomous University of Queretaro is a Mexican public university based in the city of Santiago de Querétaro, Querétaro, but with campuses around the state. The main campus is located in Cerro de las Campanas, but there are campuses is Amealco, Cadereyta, Jalpan, Juriquilla and San Juan del Río. Wikipedia.
Garg N.,University of Illinois at Urbana - Champaign |
Salazar-Ocampo L.M.A.,Autonomous University of Queretaro |
Van Der Donk W.A.,University of Illinois at Urbana - Champaign
Proceedings of the National Academy of Sciences of the United States of America | Year: 2013
The biosynthesis of several classes of ribosomally synthesized and posttranslationally modified peptides involves dehydration of serine and threonine residues. For class I lantibiotics, thiopeptides, and goadsporin, this dehydration is catalyzed by lanthionine biosynthetic enzyme B (LanB) or LanB-like proteins. Although LanB proteins have been studied since 1992, in vitro reconstitution of their dehydration activity has been elusive. We show here the in vitro activity of the dehydratase involved in the biosynthesis of the food preservative nisin (NisB). In vitro, NisB dehydrated its substrate peptide NisA eight times in the presence of glutamate, ATP, Mg2+, and the ribosomal/membrane fraction of bacterial cell extract. Mutation of 23 highly conserved residues of NisB identified a number of amino acids that are essential for dehydration activity. In addition, these mutagenesis studies identified three mutants, R786A, R826A, and H961A, that result in multiple glutamylations of the NisA substrate. Glutamylation was observed during both Escherichia coli coexpression of NisA with these mutants and in vitro assays. Treatment of the glutamylated substrate with WT NisB results in dehydrated NisA, suggesting that the glutamylated peptide is an intermediate in dehydration. Collectively, these studies suggest that dehydration involves glutamylation of the side chains of Ser and Thr followed by elimination. The latter step has precedent in the virginiamycin resistance protein virginiamycin B lyase. These studies will facilitate investigation of other LanB proteins involved in the biosynthesis of lantibiotics, thiopeptides, and goadsporin.
Amezquita-Sanchez J.P.,Autonomous University of Queretaro |
Adeli H.,Ohio State University
Archives of Computational Methods in Engineering | Year: 2016
Signal processing is the key component of any vibration-based structural health monitoring (SHM). The goal of signal processing is to extract subtle changes in the vibration signals in order to detect, locate and quantify the damage and its severity in the structure. This paper presents a state-of-the-art review of recent articles on signal processing techniques for vibration-based SHM. The focus is on civil structures including buildings and bridges. The paper also presents new signal processing techniques proposed in the past few years as potential candidates for future SHM research. The biggest challenge in realization of health monitoring of large real-life structures is automated detection of damage out of the huge amount of very noisy data collected from dozens of sensors on a daily, weekly, and monthly basis. The new methodologies for on-line SHM should handle noisy data effectively, and be accurate, scalable, portable, and efficient computationally. © 2014, CIMNE, Barcelona, Spain.
Yahia E.M.,Autonomous University of Queretaro |
Mondragon-Jacobo C.,Instituto Nacional de Investigaciones Nucleares
Food Research International | Year: 2011
Cactus pear fruit (Opuntia) are harvested from various species of the genus Opuntia of the cactus family (Cactaceae), and are produced and consumed in several countries. We have characterized the nutritional content and antioxidant capacity (AC) of the fruit of ten cultivars/lines of distinct pulp colors. 'Camuesa' had the highest betalains, total carotenoids, ß-carotene, ascorbic acid, and was one of the highest in total phenolic compounds, but its AC did not demonstrate outstanding differences with some other cultivars/lines that were not as rich in these compounds. 'Roja Pelota' had high AC when measured with the DPPH assay, but had low total carotenoids, ß-carotene and total phenolic content. 'Reyna' had slightly low AC as measured by the FRAP assay, practically no betalains, and low vitamin C, tocopherols, ß-carotene, and total phenolic content. © 2011 Elsevier Ltd.
Valtierra-Rodriguez M.,Autonomous University of Queretaro |
De Jesus Romero-Troncoso R.,University of Guanajuato |
Osornio-Rios R.A.,University of Guanajuato |
Garcia-Perez A.,Autonomous University of Queretaro
IEEE Transactions on Industrial Electronics | Year: 2014
The detection and classification of power quality (PQ) disturbances have become a pressing concern due to the increasing number of disturbing loads connected to the power line and the susceptibility of certain loads to the presence of these disturbances; moreover, they can appear simultaneously since, in any real power system, there are multiple sources of different disturbances. In this paper, a new dual neural-network-based methodology to detect and classify single and combined PQ disturbances is proposed, consisting, on the one hand, of an adaptive linear network for harmonic and interharmonic estimation that allows computing the root-mean-square voltage and total harmonic distortion indices. With these indices, it is possible to detect and classify sags, swells, outages, and harmonics-interharmonics. On the other hand, a feedforward neural network for pattern recognition using the horizontal and vertical histograms of a specific voltage waveform can classify spikes, notching, flicker, and oscillatory transients. The combination of the aforementioned neural networks allows the detection and classification of all the aforementioned disturbances even when they appear simultaneously. An experiment under real operating conditions is carried out in order to test the proposed methodology. © 2013 IEEE.
Sandoval-Oliveros M.R.,Autonomous University of Queretaro |
Journal of Agricultural and Food Chemistry | Year: 2013
Chia (Salvia hispanica L.) is a plant that produces seeds rich in some nutraceutical compounds with high protein content, but little is known about them; for this reason the aim of this study was to characterize the seed storage proteins. Protein fractions were extracted from chia seed flour. The main protein fraction corresponded to globulins (52%). Sedimentation coefficient studies showed that the globulin fraction contains mostly 11S and 7S proteins. The molecular sizes of all the reduced fractions were about 15-50 kDa. Electrophoretic experiments under native conditions exhibited four bands of globulins in the range of 104-628 kDa. The denaturation temperatures of crude albumins, globulins, prolamins, and glutelins were 103, 105, 85.6, and 91 C, respectively; albumins and globulins had relatively good thermal stability. Selected globulin peptides by mass spectrometry showed homology to sesame proteins. A good balance of essential amino acids was found in the seed flour and globulins, especially of methionine+cysteine. © 2012 American Chemical Society.
Munoz-Huerta R.F.,Autonomous University of Queretaro
Sensors (Basel, Switzerland) | Year: 2013
Nitrogen (N) plays a key role in the plant life cycle. It is the main plant mineral nutrient needed for chlorophyll production and other plant cell components (proteins, nucleic acids, amino acids). Crop yield is affected by plant N status. Thus, the optimization of nitrogen fertilization has become the object of intense research due to its environmental and economic impact. This article focuses on reviewing current methods and techniques used to determine plant N status. Kjeldahl digestion and Dumas combustion have been used as reference methods for N determination in plants, but they are destructive and time consuming. By using spectroradiometers, reflectometers, imagery from satellite sensors and digital cameras, optical properties have been measured to estimate N in plants, such as crop canopy reflectance, leaf transmittance, chlorophyll and polyphenol fluorescence. High correlation has been found between optical parameters and plant N status, and those techniques are not destructive. However, some drawbacks include chlorophyll saturation, atmospheric and soil interference, and the high cost of instruments. Electrical properties of plant tissue have been used to estimate quality in fruits, and water content in plants, as well as nutrient deficiency, which suggests that they have potential for use in plant N determination.
Garcia O.P.,Autonomous University of Queretaro
Proceedings of the Nutrition Society | Year: 2012
Obesity has been associated with low-grade systemic inflammation and with micronutrient deficiencies. Obese individuals have been found to have lower vitamin A levels and lower vitamin A intake compared with normal-weight individuals. Vitamin A plays a major role in the immune function, including innate immunity, cell-mediated immunity and humoral antibody immunity. It has also been recognised recently that vitamin A has important regulatory functions. Vitamin A status has an important effect on the chronic inflammatory response. Vitamin A deficiency increases a T-helper type 1 (Th1) response, elevates levels of pro-inflammatory cytokines, increases the expression of leptin, resistin and uncoupling proteins (UCP) and promotes adipogenesis. The effect of vitamin A deficiency on obesity might be increasing the risk of fat deposition and also the risk of chronic inflammation associated with obesity. Supplementation with vitamin A in vitro and in animal models has been found to reduce concentrations of adipocytokines, such as leptin and resistin. In conclusion, vitamin A deficiency increases a Th1 response in the presence of obesity and thus, increases the inflammatory process involved in chronic inflammation and fat deposition. The metabolism of leptin and other adipocytokines may play a critical role in the effect of vitamin A deficiency in the inflammatory response observed in obesity. © 2012 The Author.
Jauregui-Correa J.C.,Autonomous University of Queretaro
Mechanical Systems and Signal Processing | Year: 2013
The effect of the housing stiffness on nonlinear traveling waves is presented in this work. It was found that the housing controls the synchronization of nonlinear elements and it allows nonlinear waves to travel through the structure. This phenomenon was observed in a gearbox with a soft housing, and the phenomenon was reproduced with a lump-mass dynamic model. The model included a pair of gears, the rolling bearings and the housing. The model considered all the nonlinear effects. Numerical and experimental results were analyzed with a time-frequency method using the Morlet wavelet function. A compound effect was observed when the nonlinear waves travel between the gears and the bearings: the waves increased the dynamic load amplitude and add another periodic load. © 2012 Published by Elsevier Ltd.
Gutierrez-Samperio C.,Autonomous University of Queretaro
Gaceta Medica de Mexico | Year: 2016
We reviewed the literature covering the medical movement in 1964-1965, which began on November 26, 1964 with the ISSSTE November 20 Hospital scholarship protest and the emergence of the Mexican Resident Interns Physicians Association (Asociación Mexicana de Médicos Residentes e Internos, AMMRI) and the Mexican Medical Alliance (Alianza de Médicos Mexicanos, AMM). We describe four work stoppages, two protest marches, the takeover of hospitals by the police, four interviews with Licenciado Gustavo Diaz Ordaz and his first presidential report. After that, attacks in the press, harassment, and repression provoked the weakening of the movement that ended in the AMM assembly on January 18, 1966. © 2016, Academia Nacional de Medicina. All rights reserved.
CSIC - Institute of Refrigeration and Autonomous University of Queretaro | Date: 2012-10-17
The invention relates to micro-, submicro- or nano-structures comprising amaranth protein, optionally combined with at least one other biopolymer, which structures are suitable for use as an encapsulation matrix. In particular, the invention relates to micro-, submicro- or nano-structures comprising amaranth protein and a polysaccharide. The invention also relates to the production method thereof, said method comprising an electrospinning, electrospraying or blow spinning step. The encapsulated product is characterised in that it comprises an encapsulation matrix formed by micro-, submicro- or nano-structures of the invention and at least one functional ingredient. The invention further relates to the method for obtaining same.