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Târgovişte, Romania

Stoica A.,University Valahia of Targoviste | Hossu A.-M.,University Valahia of Targoviste | Barascu E.,University Valahia of Targoviste | Iordan M.,University Valahia of Targoviste | Maria M.-F.,Authority of Public Health
Revista de Chimie | Year: 2010

The use of single glucose oxidase or fungal α-amylase is a solution to improve the performances of the weak wheat flours. Both glucose oxidase and fungal αa-amylase have a strengthening action for the poor gluten network, increasing the capacity of wheat flour doughs to retain the fermentation gases. The aim of this study is to propose a new variant to improve the quality of bread made from these flours by their supplementation with a combination of these two enzymes. White flour used was analyzed by determination of several quality indicators, the results indicating that flour has a fragile protein network and, moreover, a very low α-amylase activity. Using the baking tests it was determined how the use of the two fungal enzymes combinations causes a substantial improvement of bread quality indicators such as volume, porosity and elasticity, clearly superior to their individual use. For a better comparison, it was calculated a score for the physical properties of bread. When the two enzymes were used in combination, a synergistic effect was observed as a result of cooperation of the two enzymes in the dough. It could be noticed, also, the improvement of the external appearance of bread crust obtained by combining the two enzymes in comparison with the case of using only glucose oxidase.

Maria M.-F.,Authority of Public Health | Hossu A.-M.,University Valahia of Targoviste | Iovi A.,Polytechnic University of Timisoara | Negrea P.,Polytechnic University of Timisoara | Stoica A.,University Valahia of Targoviste
Revista de Chimie | Year: 2010

The determination of chromium in most samples is extremely difficult because of very low levels present. The only widely available analytical method with sufficient sensitivity is graphite furnace atomic absorption spectrometry (GFAAS), yet values reported in the early literature were divergent. This paper describes a new analytical method to estimate occupational exposure levels of total chromium in workplace atmospheres by atomic absorption spectrometry with a background correction using a cellulosic membrane for sample and solubilization through wet mineralization with nitric acid. The atomisation temperature used was 2500°C and a cleaning step for graphite furnace after each samples serie was realized. The validation of GFAAS method was made in concordance with International Conference on Harmonization (ICH). Standard calibration solutions were used for the range 10-100 μg/L and they were used in calculating the performance parameters for this method. The performance characteristics established during the "in-house" validation of the method indicate that it gives good information about mobility of total chromium in workplace atmospheres and it was validated. Determinations were undertaken over a three-year period in different points of a chroming room and led to different results for the total chromium concentration, ranging between 0.0018-0.051 mg/m3.

Hossu A.-M.,University Valahia of Targoviste | Maria M.-F.,Authority of Public Health | Stoica A.,University Valahia of Targoviste | Barascu E.,University Valahia of Targoviste
Revista de Chimie | Year: 2014

Food and beverage contamination by lead and cadmium occurs either in direct way or by contact with packaging, serving, cooking and/or store materials. In this study, we were interested in lead and cadmium migration into food in contact with ceramicwares. The quantities of these transferred from ceramic articles shall not exceed the limits: 0.3mg/L for cadmium and 4 mg/L for lead for objects can be filled, with a volume smaller than 3 liters, in accordance with European Directive 84/500/EEC. The analytical application of GFAAS method was tested with samples from Romania and China.

Berglund M.,Karolinska Institutet | Larsson K.,Karolinska Institutet | Grander M.,Karolinska Institutet | Casteleyn L.,Catholic University of Leuven | And 49 more authors.
Environmental Research | Year: 2015

The metal cadmium (Cd) is a widespread environmental pollutant with documented adverse effects on the kidneys and bones from long-term environmental exposure, but with insufficiently elucidated public health consequences such as risk of cardiovascular disease, hormone-related cancer in adults and developmental effects in children. This study is the first pan-European human biomonitoring project that succeeded in performing harmonized measurements of Cd in urine in a comparable way in mother-child couples from 16 European countries. The aim of the study was to evaluate the overall Cd exposure and significant determinants of Cd exposure.A study population of 1632 women (24-52 years of age), and 1689 children (5-12 years of age), from 32 rural and urban areas, was examined within a core period of 6 months in 2011-2012. Women were stratified as smokers and non-smokers. As expected, smoking mothers had higher geometric mean (gm) urinary cadmium (UCd; 0.24 μg/g crea; n=360) than non-smoking mothers (gm 0.18 μg/g crea; n=1272; p<0.0001), and children had lower UCd (gm 0.065 μg/g crea; n=1689) than their mothers at the country level. Non-smoking women exposed to environmental tobacco smoke (ETS) at home had 14% (95% CI 1-28%) higher UCd than those who were not exposed to ETS at home (p=0.04). No influence of ETS at home or other places on UCd levels was detected in children. Smoking women with primary education as the highest educational level of the household had 48% (95% CI 18-86%) higher UCd than those with tertiary education (p=0.0008). The same observation was seen in non-smoking women and in children; however they were not statistically significant. In children, living in a rural area was associated with 7% (95% CI 1-13%) higher UCd (p=0.03) compared to living in an urban area. Children, 9-12 years had 7% (95% CI 1-13%) higher UCd (p=0.04) than children 5-8 years.About 1% of the mothers, and 0.06% of the children, exceeded the tolerable weekly intake (TWI) appointed by EFSA, corresponding to 1.0 μg. Cd/g crea in urine. Poland had the highest UCd in comparison between the 16 countries, while Denmark had the lowest. Whether the differences between countries are related to differences in the degree of environmental Cd contamination or to differences in lifestyle, socioeconomic status or dietary patterns is not clear. © 2014 The Authors.

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