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Ferguson J.C.,University of Queensland | O'Donnell C.C.,University of Queensland | Chauhan B.S.,University of Queensland | Adkins S.W.,University of Queensland | And 7 more authors.
Crop Protection | Year: 2015

Spray drift is a consideration for growers and applicators who are increasingly selecting larger droplet producing nozzles to allay their concerns. As new technologies arrive on the market, the prices of individual nozzles have risen which puts a greater need for consistency among nozzles to be worth the investment. These nozzles, while effective at reducing spray drift, may not always be consistent at maintaining efficacy which can be a result of a lack of uniformity in the production of these nozzles. Twenty-one spray drift reducing nozzles were compared for droplet size distributions across three liquids of varying dynamic surface tensions in a wind tunnel at the University of Queensland. Research sought to identify the repeatability of each nozzle type by randomly selecting five units to test consistency of droplet size measurements across nozzle type. Results indicate that some nozzle types are consistent and repeatable while others are not. It was also observed that some nozzle types are relatively unaffected by liquid type, where others resulted in a droplet size change in volume median diameter (VMD) of 100 μm depending on liquid type at the same operating pressure. Research from this study will help growers and industry to select the best nozzle types to ensure uniformity of application, to maximize efficacy and to reduce pesticide spray drift. © 2015 Elsevier Ltd.


Wang Y.,Australian Institute for Bioengineering and Nanotechnology AIBN | Vaidyanathan R.,Australian Institute for Bioengineering and Nanotechnology AIBN | Shiddiky M.J.A.,Australian Institute for Bioengineering and Nanotechnology AIBN | Trau M.,Australian Institute for Bioengineering and Nanotechnology AIBN | Trau M.,University of Queensland
ACS Nano | Year: 2015

A rapid and simple approach is presented to address two critical issues of surface-enhanced Raman scattering (SERS)-based immunoassay such as removal/avoiding nonspecific adsorption and reducing assay time. The approach demonstrated involves rationally designed fluorophore-integrated gold/silver nanoshells as SERS nanotags and utilizes alternative current electrohydrodynamic (ac-EHD)-induced nanoscaled surface shear forces to enhance the capture kinetics. The assay performance was validated in comparison with hydrodynamic flow and conventional immunoassay-based devices. These nanoscaled physical forces acting within nanometer distances from the electrode surface enabled rapid (40 min), sensitive (10 fg/mL), and highly specific detection of human epidermal growth factor receptor 2 in breast cancer patient samples. We believe this approach presents potential for the development of rapid and sensitive SERS immunoassays for routine clinical diagnosis. © 2015 American Chemical Society.


Wang Y.,Australian Institute for Bioengineering and Nanotechnology AIBN | Wee E.J.H.,Australian Institute for Bioengineering and Nanotechnology AIBN | Trau M.,Australian Institute for Bioengineering and Nanotechnology AIBN | Trau M.,University of Queensland
Chemical Communications | Year: 2016

Sensitive and accurate total genomic DNA methylation analysis was demonstrated by surface-enhanced Raman scattering (SERS) via embedded internal SERS nanotags. The assay was sensitive to 0.2 ng input DNA while differentiating as low as 6.25% changes in DNA methylation. The method could also successfully differentiate cells before and after de-methylating drug-treatment and between tumor and normal biopsies. © The Royal Society of Chemistry 2016.


Wang Y.,Australian Institute for Bioengineering and Nanotechnology AIBN | Wee E.J.H.,Australian Institute for Bioengineering and Nanotechnology AIBN | Trau M.,Australian Institute for Bioengineering and Nanotechnology AIBN | Trau M.,University of Queensland
Chemical Communications | Year: 2015

Sensitive and accurate DNA methylation analysis at CpG resolution was demonstrated using surface-enhanced Raman scattering (SERS) via ligase chain reaction (LCR). The method was sensitive to 10% changes in methylation and the accuracy of methylation estimates in cells and serum DNA validated with sequencing. The LCR/SERS approach may have broad applications as an alternative (epi)genetic detection method. © 2015 Royal Society of Chemistry.


Wang Y.,Australian Institute for Bioengineering and Nanotechnology AIBN | Wang Y.,University of Duisburg - Essen | Rauf S.,Australian Institute for Bioengineering and Nanotechnology AIBN | Grewal Y.S.,Australian Institute for Bioengineering and Nanotechnology AIBN | And 6 more authors.
Analytical Chemistry | Year: 2014

Quantitative and accurate detection of multiple biomarkers would allow for the rapid diagnosis and treatment of diseases induced by pathogens. Monoclonal antibodies are standard affinity reagents applied for biomarkers detection; however, their production is expensive and labor-intensive. Herein, we report on newly developed nanoyeast single-chain variable fragments (NYscFv) as an attractive alternative to monoclonal antibodies, which offers the unique advantage of a cost-effective production, stability in solution, and target-specificity. By combination of surface-enhanced Raman scattering (SERS) microspectroscopy using glass-coated, highly purified SERS nanoparticle clusters as labels, with a microfluidic device comprising multiple channels, a robust platform for the sensitive duplex detection of pathogen antigens has been developed. Highly sensitive detection for individual Entamoeba histolytica antigen EHI115350 (limit of detection = 1 pg/mL, corresponding to 58.8 fM) and EHI182030 (10 pg/mL, corresponding 453 fM) with high specificity has been achieved, employing the newly developed corresponding NYscFv as probe in combination with SERS microspectroscopy at a single laser excitation wavelength. Our first report on SERS-based immunoassays using the novel NYscFv affinity reagent demonstrates the flexibility of NYscFv fragments as viable alternatives to monoclonal antibodies in a range of bioassay platforms and paves the way for further applications. © 2014 American Chemical Society.

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