Li Z.-Y.,Atom HighTech Co.
Chinese Journal of Biologicals | Year: 2013
Objective: To express human creatine kinase MB isoenzyme (CK-MB) in prokaryotic cells and purify the expressed product. Methods: CK-M and CK-B genes were amplified from plasmids SC319293 and SC119007 respectively and inserted into prokaryotic expression vector pGEX-4T-1 with GST tag. The constructed recombinant plasmid pGEX-4T-1-CK-MB was transformed to E coli. BL21(DE3) for expression under induction of IPTG. The expressed fusion protein was purified by GST affinity chromatography and determined for antigenicity by ELISA. Results: PCR, restriction analysis and sequencing proved that recombinant plasmid pGEX-4T-1-CKMB was constructed correctly. The expressed recombinant fusion protein, with a relative molecular mass of about 110 000, contained 57% of total somatic protein, mainly existed in a form of inclusion body, reached a purity of more than 90%, and showed specific binding to rabbit polycolonal antibodies against CK-M and CK-B. Conclusion: Recombinant CK-MB protein was successfully expressed in prokaryotic cells and purified, which laid a foundation of large-scale production of the protein.
Ding X.-J.,China Institute of Atomic Energy |
Ding X.-J.,Atom HighTech Co. |
Guan G.-Y.,China Institute of Atomic Energy |
Guan G.-Y.,Atom HighTech Co. |
And 5 more authors.
He-Huaxue yu Fangshe Huaxue/Journal of Nuclear and Radiochemistry | Year: 2014
By optimizing conditions experiments of styrene-butadiene-styrene block copolymer (SBS) content, cosolvent type and dosage, processing temperature, processing time, the radiation dose rate and absorbed dose, the preparation methods of SBS modified asphalt, 60Co irradiated SBS and irradiation SBS modified asphalt have been studied; by testing the performance index of modified asphalt cement it is to evaluate the preparation methods. The experimental results show that the mass fraction of SBS is 15%-20%, the mass fraction of cosolvent is 15%-30%, blending, then at 100-165℃, 100-200 r/min mechanical agitation 20-30 min, at 180-210℃, 100-200 r/min mechanical agitation 90-140 min; or at 120-160℃, 100-200 r/min mechanical agitation 80-100 min, in these conditions the modified asphalt cement is better; the asphalt cement modified by SBS which was irradiated by gamma rays from a 60Co source in vacuum at 0.75 kGy/h, 30 kGy have good performance.
Liu J.,Tianjin Medical University |
Zheng L.,Atom Hightech Co. |
Zhang W.,Tianjin Union Medicine Center |
Chang K.,Tianjin Medical University |
Pang Y.,Tianjin Union Medicine Center
Indian Journal of Palliative Care | Year: 2013
Aims: In this paper, we have reported a previously undescribed risk factor of deterioration of renal function in zoledronic acid treatment of skeletal metastasis - high serum calcium level. Based on this consideration, a modified method of treatment of hypercalcemia (HCM) with zoledronic acid is suggested in this paper. Material and Methods: Bone scan findings of 1090 cancer patients were analyzed, of which 26 had intense renal parenchymal uptake as a result of HCM or bone metastases. Subsequently, a total of 56 bone metastases patients with zoledronic acid treatment were divided into three groups: HCM group who were pre-treated to normal serum calcium level (13 patients), HCM group (19 patients), and normal serum calcium group (24 patients). Results: More patients with intense renal parenchymal uptake were hyperglycemic, statistically significantly (18/26 versus 19/1064, P = 2.1, E-78). No more patients with intense renal parenchymal uptake were associated with bone metastases (14/26 versus 438/1064, P = 0.20). Subsequently, more HCM patients receiving zoledronic acid treatment showed renal injury compared to patients with normal serum calcium level (5/15 versus 2/24, P < 0.05) and HCM patients with pre-treatment to normal serum calcium level (5/15 versus 1/17, P < 0.05). Conclusions: Intense renal parenchymal uptake of bisphosphonates is closely related to HCM rather than to bone metastases in cancer patients. The serum calcium should be measured and reduced to normal level before zoledronic acid is used in managements of adverse skeletal events in order to decrease the risk of renal injury. Copyright © Indian Journal of Palliative Care 2013.