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Garala K.C.,Atmiya Institute of Pharmacy | Shah P.H.,University of Aberdeen
Journal of Macromolecular Science, Part A: Pure and Applied Chemistry | Year: 2010

The present work was designed to develop suitable transdermal matrix patches using the polymer blends of hydroxy propyl methyl cellulose (HPMC) and Eudragit RL100 (ERL) with triethyl citrate as a plasticizer in group A and in group B, other than HPMC and ERL, crosslinking agent, succinic acid was added. A 32 full factorial design was employed for both groups. The concentration of HPMC and ERL were used as independent variables, while percentage drug release was selected as dependent variable. Physical evaluation was performed such as moisture content, moisture uptake, tensile strength, flatness and folding endurance. In vitro diffusion studies were performed using cellulose acetate membrane (pore size 0.45 ) in a Franz's diffusion cell. The concentration of diffused drug was measured using UV-visible spectrophotometer (V-530, Jasco) at max 272 nm. The experimental results shows that the transdermal drug delivery system (TDDS) containing ERL in higher proportion gives sustained the release of drug and patches containing crosslinking agent shows more release than those do not contains succinic acid. Source

Patel M.B.,Atmiya Institute of Pharmacy
Journal of complementary & integrative medicine | Year: 2012

The present investigation was aimed at discovery of novel acetylcholinesterase (AChE) inhibitors. In vitro AchE inhibitory activity of various extracts of Sphaeranthus indicus flower heads was carried out. The petroleum ether fraction of S. indicus flowers (SIPE) exhibited significant activity. The fraction was found to be rich in sesquiterpene lactone content possibly responsible for in vitro AChE inhibition. In further study, the antiamnesic activities of SIPE in mice on the learning and memory impairments induced by scopolamine (1.0 mg/kg, i.p.) were examined. SIPE (10 mg/kg, p.o.) administration significantly reversed cognitive impairments in mice by passive avoidance test (P < 0.05). It also reduced escape latencies in training trials and prolonged swimming times in the target quadrant during the probe trial in the water maze task (P < 0.05). These results indicated that S. indicus due to its sesquiterpene lactones have anti-cholinesterase activity. A major sesquiterpene lactone, 7-hydroxy frullanolide along with other constituents were isolated from SIPE and evaluated for AchE inhibitory activity. Negative results were obtained in case of isolated compounds. Synergistic effect between constituents of SIPE was confirmed to have anti-amnesic activities that may be useful for cognitive impairment treatment. Source

Patel M.B.,Atmiya Institute of Pharmacy | Mishra S.M.,M. S. University of Baroda
Journal of Functional Foods | Year: 2012

Antidiabetic potential of Tinospora cordifolia stem is well proven. In the course of screening of useful α-glucosidase inhibitors, we prepared alkaloid fraction (AFTC) and isolated three isoquinoline alkaloids, namely, jatrorrhizine, palmatine and magnoflorine as active candidates for α-glucosidase inhibition. The enzyme kinetics was studied using sucrose and maltose as substrates. Michaelis-Menten constant (K m) and maximal velocity (V max) values were estimated. A significant decrease in V max and unaltered K m was observed in case of jatrorrhizine and palmatine (non-competitive inhibition). Magnoflorine was found to increase apparent K m and shown to be reversible, competitive inhibition. The IC 50 value as sucrase inhibitor was 36.25, 23.46 and 9.8μg/mL for jatrorrhizine, palmatine and magnoflorine, respectively, and as maltase inhibitor was 22.05, 38.42 and 7.6μg/mL for jatrorrhizine, palmatine and magnoflorine, respectively. In vivo studies were conducted on rats to determine oral glucose tolerance test (OGTT), using different substrates: glucose, sucrose and maltose. The increase in plasma glucose level was significantly suppressed (P<0.01) by all the three alkaloids at 20mg/kg b.w. Magnoflorine possessed the most potential activity as α-glucosidase inhibitor in vitro and in vivo. © 2011 Elsevier Ltd. Source

Gondalia R.P.,Atmiya Institute of Pharmacy | Dharamsi A.P.,Pioneer India
Asian Journal of Pharmaceutical and Clinical Research | Year: 2011

A binary mixture of Naproxen sodium and Sumatriptan succinate was determined by UV spectroscopic methods. The method involved determination of NAP and SUMA using the first derivative spectrophotometry at 241.0 and 236.0 nm over the concentration ranges of 0.5-3.5 μg/ml for both. The methods were successively applied to pharmaceutical formulation because no Spectroscopic interferences from the tablet excipients were found. The suitability of these methods for the quantitative determination of the compounds was proved by validation. Source

Patel M.,Atmiya Institute of Pharmacy | Mishra S.,M. S. University of Baroda
Acta Chromatographica | Year: 2012

Methods based on RP-HPLC and HPTLC with UV detection for rapid quantitative determination of marker component in Enicostemma hyssopifolium, swertisin are described. The recovery of the compound was between 96.3-101.5% by HPTLC method and 98.9-100.2% by HPLC assay. The relative standard deviations ranged between 1.53-1.81 (intra-day) and 1.33-1.96 (inter-day) for HPTLC and 0.60-1.11 (intra-day) and 0.80-1.20 (inter-day) for HPLC. The methods were used for routine analysis of swertisin in the aerial parts of the plant. HPTLC method was found to be more economic and less time consuming than HPLC. Reproducibility and recovery in HPLC method were better than that in HPTLC method. Source

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