Entity

Time filter

Source Type


Saeed I.K.,Central Veterinary Research Laboratory | Ali Y.H.,Central Veterinary Research Laboratory | AbdulRahman M.B.,Central Veterinary Research Laboratory | Mohammed Z.A.,Central Veterinary Research Laboratory | And 4 more authors.
Tropical Animal Health and Production | Year: 2015

This study was intended to determine the role played by peste des petits ruminants (PPR) in causing respiratory infections in camels and its association with other respiratory viruses. A total of 474 lung specimens showing pneumonia were collected from clinically healthy camels in slaughterhouses at five different areas in Sudan. Using immunocapture ELISA (IcELISA), 214 specimens (45.1 %) were found to be positive for PPR antigen. The highest prevalence was found in central Sudan (59.9 %) then northern Sudan (56.6 %) and eastern Sudan (26.6 %). Parainfluenza virus 3 (PIV 3), respiratory syncytial virus (RSV), bovine herpes virus-1 (BHV-1), bovine viral diarrhea (BVD), and adenovirus were detected in 4.4, 2.9, 2.0, 9.0, and 1.3 % of the specimens, respectively. PPR antigen was found in about 50 % of specimens that showed positive result for other viral antigens. Twenty-five of 28 BVD, 15 of 16 PIV3, 8 of 12 RSV, 4 of 4 adenovirus, and 4 of 5 BHV-1 were found in association with other respiratory antigens. Results revealed the existence of PPRV infection in dromedary camels in Sudan and present evidence for mixed virus infection, suggesting that respiratory infections in camels might be exacerbated by PPRV. © 2015, Springer Science+Business Media Dordrecht. Source


Kwiatek O.,Control of Exotic and Emerging Animal Diseases | Ali Y.H.,Central Veterinary Research Laboratories | Saeed I.K.,Central Veterinary Research Laboratories | Khalafalla A.I.,University of Khartoum | And 12 more authors.
Emerging Infectious Diseases | Year: 2011

Interest in peste des petits ruminants virus (PPRV) has been stimulated by recent changes in its host and geographic distribution. For this study, biological specimens were collected from camels, sheep, and goats clinically suspected of having PPRV infection in Sudan during 2000-2009 and from sheep soon after the first reported outbreaks in Morocco in 2008. Reverse transcription PCR analysis confirmed the wide distribution of PPRV throughout Sudan and spread of the virus in Morocco. Molecular typing of 32 samples positive for PPRV provided strong evidence of the introduction and broad spread of Asian lineage IV. This lineage was defined further by 2 subclusters; one consisted of camel and goat isolates and some of the sheep isolates, while the other contained only sheep isolates, a finding with suggests a genetic bias according to the host. This study provides evidence of the recent spread of PPRV lineage IV in Africa. Source


Intisar K.S.,Central Veterinary Research Laboratory | Ali Y.H.,Central Veterinary Research Laboratory | Khalafalla A.I.,University of Khartoum | Taha K.M.,Atbara Veterinary Research Laboratory | Rahman M.E.A.,Central Veterinary Research Laboratory
African Journal of Microbiology Research | Year: 2010

The incidence of adenovirus type 3 infections in camels in Sudan was studied. Lungs of Camel with pneumonia lungs (n = 239) were collected from slaughter houses at four different areas in Sudan. Adenovirus type 3 antigen was detected in 1.3% of 239 tested camel lungs by the use of sandwich ELISA. Specimens from Northern (3.3%) and Central Sudan (1.2%) were found to be positives. Direct fluorescent antibody test (FAT) was used to confirm the adenovirus ELISA positives; all ELISA positives were found to be positive using FAT. Seroprevalence of adenovirus type 3 was investigated, camel sera (n = 260) were collected from the same areas in Sudan. Collected sera were examined for adenovirus antibodies using indirect ELISA. The overall detected seroprevalence was 90%; highest prevalence was in South Central (100%) then Western (94.3%) and Central Sudan (92.5%). The lowest seroprevalence was in Northern Sudan (80%). The most detected degree of positivity was +3 then +5. This represents the first report for the detection of adenovirus type 3 antigen and antibodies in camels in Sudan. It was noticed to cause pathologic effect in camel lungs. © 2010 Academic Journals. Source


Ahmed B.M.,Nile Valley University | Taha K.M.,Atbara Veterinary Research Laboratory | Enan K.A.,Central Laboratory | Elfahal A.M.,Central Laboratory | El Hussein A.R.M.,Animal Resources Research Corporation
Vaccine | Year: 2013

Malignant ovine theileriosis caused by Theileria lestoquardi is an economically important disease infecting small ruminants in the Sudan. The disease causes massive losses among sheep in many regions of Northern Sudan. The present studies were done to isolate lymphoblastiod cells infected with malignant ovine theileriosis and attenuate them by passage using culture media to develop and produce schizonts candidate vaccine, then test its efficacy and safety by exposing immunized lambs to field challenge in an area endemic with T. lestoquardi. In the present experiments we isolated and established an in vitro culture of T. lestoquardi infected lymphoblast cell line. Long-term culture of T. lestoquardi infected lymphoplastoid cells was shown to result in attenuation of their virulence and lambs inoculated with different doses of such cells at passage 105 exhibited very mild reactions with fever that lasted for 1-5 days and parasitaemia of <0.2%. The experimental lambs immunized with this candidate vaccine were immune and protected when exposed to field challenge in an area endemic of ovine theileriosis, while morbidity and mortality among non-immunized animals reached 76.9% and 46.15%, respectively, and they exhibited the clinical signs of malignant ovine theileriosis that included, high fever, loss of appetite, enlargement of lymph nodes, jaundice, loss of weight and death. The present study demonstrates the efficacy and the safety of this attenuated cell line as a live attenuated candidate vaccine. © 2013 Elsevier Ltd. Source


Intisar K.S.,Central Veterinary Research Laboratory | Ali Y.H.,Central Veterinary Research Laboratory | Khalafalla A.I.,University of Khartoum | Mahasin E.A.R.,Central Veterinary Research Laboratory | And 2 more authors.
Tropical Animal Health and Production | Year: 2010

The role of pestivirus particularly bovine viral diarrhea virus (BVDV) in causing respiratory infections in camels was studied in four different localities in Sudan. The evaluation was carried out using ELISA, and positive specimens were further tested using direct fluorescent antibody technique (FAT) and reverse transcriptase polymerase chain reaction (RT-PCR) for confirmation. The overall detected seroprevalence of BVD in camel sera was 84.6% with the highest prevalence in Western Sudan (92.5%) and with most of positives showing 2+ and 3+ titer. Out of 186 lung specimens examined for BVDV antigen, 13 were found positive (7%) with the highest prevalence in Central Sudan. All ELISA-positive specimens were positive using FAT and RT-PCR. To our knowledge, this is the first report for the detection of BVDV antigen and antibodies in camels in Sudan. © 2010 Springer Science+Business Media B.V. Source

Discover hidden collaborations