Astrakhan', Russia

Astrakhan State Medical Academy

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Chernysheva E.N.,Astrakhan State Medical Academy | Panova T.N.,Astrakhan State Medical Academy
Russian Open Medical Journal | Year: 2014

The purpose of the work is to research siofor influence (metformin) on the activity of the process of lipid peroxidation and antioxidant activity of blood serum at patients with metabolic syndrome. Material and Methods - 62 patients with metabolic syndrome at the age from 30 till 60 were examined and treated by siofor (1700 mg per day) during a year. The process of lipid peroxidation was studied due to the level of lipid hydroperoxide of blood serum. Antioxidant capacity was based on the antioxidant reaction in the blood serum with definite number of exogenic hydrogen dioxide (mkmole/l) with the method of enzyme-linked immunosorbent assay (ELISA). Results - Intensification of process of lipid peroxidation has been observed at patients with metabolic syndrome - the level of lipid hydroperoxide of blood serum has been 2.9 (1.9, 3.9) mkM (presented as median and interquartile range), antioxidant activity of blood serum has been decreased - 276.4 (239.0, 379.9) mkmole/l. In 12 months of siofor intake hydroperoxide level has been decreased till 1.1 (0.8, 1.9) mkM, but antioxidant activity has been increased and amounted 320.0 (278.9, 334.3) mkmole/l. Conclusion - Siofor has been proved to be a highly effective medicine for correction of process of lipid peroxidation and for improvement of antioxidant activity of blood serum at patients with metabolic syndrome. © 2014, LLC Science and Innovations, Saratov, Russia. ©2014, LLC Science and Innovations, Saratov, Russia.


Velikorodov A.V.,Astrakhan State University | Ionova V.A.,Astrakhan State University | Degtyarev O.V.,Astrakhan State Medical Academy | Sukhenko L.T.,Astrakhan State University
Pharmaceutical Chemistry Journal | Year: 2013

A series of carbamate-functionalized spiro compounds were synthesized by [3+2]-cycloaddition and condensation reactions based on methyl {4(3)-[2-(2-oxo-1,2-dihydro-3H-indol-3-ylidene)acetyl]phenyl}carbamates. The antimicrobial activity with respect to E. coli, S. aureus 209, and Micrococcus and antifungal activity with respect to Microsporum canis, Trichophyton rubrum, and Candida albicans were investigated. The most active of the synthesized compounds were spiro compounds with isoxazoline and pyrazole fragments. © 2013 Springer Science+Business Media New York.


Velikorodov A.V.,Astrakhan State University | Kovalev V.B.,Astrakhan State University | Degtyarev O.V.,Astrakhan State Medical Academy | Titova O.L.,Astrakhan State Medical Academy
Pharmaceutical Chemistry Journal | Year: 2011

A series of carbamate-containing derivatives of 1,2-and 1,4-oxazine, isoxazoline, isoxazole, benzimidazole-3-oxide, and indole were obtained by [3 + 2]-cycloaddition and ring-closure reactions. The antifungal activity of the synthesized compounds was studied in vitro against Microsporum canis, Trichophyton rubrum, and Candida albicans. Among the tested compounds, the maximum antifungal activity was observed for tautomeric 5(6)-alkoxycarboxamido derivatives of 2-aryl-1-hydroxybenzimidazole-3-oxide sodium salts and 1,2-oxazines. © 2011 Springer Science+Business Media, Inc.


Tyrkov A.G.,Astrakhan State University | Abdel'rakhim M.A.,Astrakhan State University | Sukhenko L.T.,Astrakhan State University | Degtyarev O.V.,Astrakhan State Medical Academy
Pharmaceutical Chemistry Journal | Year: 2014

A series of nitrotetrazole-5-carbaldehyde hydrazones was synthesized and their antifungal activity against Candida albicans, Microsporum canis, and Trichophyton rubrum was assessed. © 2014, Springer Science+Business Media New York.


Rodionov A.A.,Astrakhan State Medical Academy | Asfandiyarov R.I.,Astrakhan State Medical Academy
Neuroscience and Behavioral Physiology | Year: 2010

The development of the epidural space was studied on 51 series of sections from embryos and early fetuses aged from four to 13 weeks using histological and embryological methods for plastic and graphic reconstruction. We found that three stages can be discriminated in the development of the epidural space: I) the primary epidural space (embryos of 16-31 mm crown-rump length (CRL); II) reduction of the primary epidural space (embryos of 35-55 mm CRL); and III) the secondary epidural space (embryos of 60-70 mm CRL and fetuses of 80-90 mm CRL). The morphogenesis of the primary epidural space is determined by the formative influence of the spinal cord and its dura mater, while that of the secondary epidural space is determined by the walls of the vertebral canal. In the spinal cord-dura mater of the spinal cord-vertebral canal correlation system, the latter two components, subjected to the inducing influence of the first, determine the morphogenesis of the epidural space via a system of morphogenetic correlations. The correlational relationships are apparent as time-linked connections between the rudiment of the dura mater of the spinal cord and the vertebral canal, resulting in the stage-by-stage formation of the epidural space in the ventrodorsal and craniocaudal directions. These same morphogenetic correlations also determine the staging of the development of the epidural space. © 2009 Springer Science+Business Media, Inc.


Pokrovsky V.I.,Central Research Institute of Epidemiology | Ugleva S.V.,Astrakhan State Medical Academy | Shabalina S.V.,Central Research Institute of Epidemiology
Terapevticheskii Arkhiv | Year: 2011

Epidemiologists register a trend in the latest decades for expansion of the areas of natural-focal transmissive fevers morbidity in the Astrakhan region. This is explained by reconfiguration of natural landscapes as a result of increasing anthropogenic impact. The Astrakhan territory exclusively contains 4 types of landscape with evolutionally formed several natural foci of transmissive fevers: Crimean hemorrhagic fever, West Nile fever, Astrakhan rickettsial fever, Q-fever. Early diagnosis of these diseases determines further treatment, prophylactic policy in the infection focus.


Ploskonos M.V.,Astrakhan State Medical Academy
Klinichescheskaya Laboratornaya Diagnostika | Year: 2014

The article analyzes comparative assessment of vitality of spermatozoa by condition of permeability of membranes for eosin and propidium iodide and comparison of results acquired using technique of light and fluorescent microscopy. The comparison of data of light microscopy with eosin staining with data of fluorescent micivscopy with propidium iodide staining demonstrated that percentage of content of spermatozoa separated from ejaculates of 28 fertile males and stained with eosin was reliably higher (34.8±3.2) than percentage of content of spermatozoa with stained with propidium iodide (21.1±4.0). After incubation of spermatozoa under room temperature during 24 hours percentage of unviable cells with stained eosin also was higher than in case of propidium iodide staining correspondingly (44.5±3.3% and 34.7±3.6%). The analysis of vitality of spermatozoa under damaging effect of oxidative stress on cell membrane developed by 4 hours incubation with 200 mkM of hydrogen peroxide (H202) demonstrated that under staining of spermatozoa with propidium iodide significantly higher percentage of damaged cells is detected. In such cases, eosin staining is less suitable for detection of vitality of spermatozoa (73.6±5.8% against 51.7±6.4%). The carried out experiment demonstrates that in case of detected effects on spermatozoa (for example, effect of oxidative stress) the light microscopy insufficiently adequate reflects degree of damage of membranes of spermatozoa. The fluorescent microscopy delects a higher percentage of spermatozoa with damaged membrane.


Alieva A.A.,Astrakhan State Medical Academy
Klinichescheskaya Laboratornaya Diagnostika | Year: 2015

The study was carried out to analyze dynamics of diaphorase and esterase activity of neutrophils of blood in patients with chronic viral hepatitis C of lower degree of activity depending on gender characteristics in dynamics of treatment. The examination and treatment were organized concerning sampling of 113 patients with chronic viral hepatitis C of lower degree of activity. The diaphorase and esterase activity of neutrophils in dynamics of treatment was detected. The analysis of diaphorases was carried out according R.P. Nartsissov technique. The content of esterase was estimated by V.M. Wachstein-F.G. Wolf technique. The count of results was implemented using Kaplow semiquantitative technique. In patients with chronic viral hepatitis C of lower degree of activity prior to treatment the activity of NAD-diaphorase was. lowered both in males and females. The activity of NADF-diaphorase prior to treatment significantly exceeded standard in males and matched standard in fernales. The application of basic therapy resulted in qualitative redistribution of cellular composition of reacting cells. All of them reacted following medium degree of activity (b). At that, average cytochemical indicator of reaction was normal in males and in females increasing of activity was observed. The activity og both diaphorases after application of complex therapy (basic therapy and cycloferon) totally returned to normality both in males and females. The esterase activity prior to treatment was decreased in males and increased in fernales (alpha-naphthylacetate esterase) and vice versa (alpha-naphthylbutyrate esterase) was increased in males and decreased infernales. After application of basic therapy in males increasing of esterase activity was registered and total redistribution of qualitative composition of reacting neutrophils (from degree "a" to degree "b"). Infernales after treatment the activity of alpha-naphthylacetate esterase was decreased and alpha-naphthylbutyrate esterase was increased. The redistribution of qualitative composition of cells was absent. The application of cycloferon brings to normality the activity of alpha-naphthylbutyrate esterase in males and activity of diaforaselpha-naphthylacetate esterase infernales. Inpatients with chronic viral hepatitis C of lower degree of activity the differences in diaphorase and esterase activity of neutrophils depending on gender characteristics in dynamics of treatment are observed.


Kasatkina S.G.,Astrakhan State Medical Academy | Panova T.N.,Astrakhan State Medical Academy
Terapevticheskii Arkhiv | Year: 2012

Aim. To study the time course changes in the parameters of endothelial dysfunction in patients with type 2 diabetes mellitus (DM) and subclinical hypothyroidism (SH) in the natural course of SH and during replacement therapy. Subjects and methods. All the examined patients were divided into 2 groups: 1) 67 patients received replacement therapy with euthyrox in a dose of 25 to 100 μg/day; 2) 60 patients were followed up. Results. At the moment of study inclusion, there was a close direct correlation between the levels of cholesterol and the aggregate intima-media thickness (IMT) (r=0.7) and between IMT and the levels of sICAM-1 (r=0.71) and sVCAM-1 (r=0.8). In the dynamics of the disease (following a year), the above correlations became weaker due to the performed treatment. A weak positive correlation was found between the aggregate IMT and the levels of sICAM-1 (/=0.2) and sVCAM-1 (r=0.3). Conclusion. In patients with type 2 DM, the presence of SH serves as an additional risk factor for endothelial dysfunction. Replacement therapy will be able to considerably retard the progression of the disease and to reduce the incidence of vascular events.


Aim: To evaluate the vascular endothelium in patients with cardiopulmonary disease, by studying the levels of endothelin-1 (ET-1) and C-type natriuretic peptide (CNP). Subjects and methods: Examinations were conducted in 212 dwellers of the Astrakhan Region, including 40 patients with chronic obstructive pulmonary disease (COPD) concurrent with hypertensive disease (HD), 40 patients with COPD concurrent with coronary heart disease (CHD), 27 somatically healthy individuals, 35 patients with Stage II HD, 35 patients with Functional Classes II and III CHD, and 35 patients with moderate and severe COPD. Results: The patients with COPD concurrent with HD and CHD were found to have endothelial dysfunction manifesting itself in the overproduction of ET-1 and CNP. The level of CNP was statistically significantly higher in the COPD + HD group than in the HD and COPD groups whereas in the COPD + HD group the level of ET-1 remained comparable to that in the COPD and HD groups. This indicates that CNP is a more sensitive indirect marker of endothelial dysfunction and that nitric oxide deficiency is aggravated in the concurrence of COPD and HD as compared to a mononosological entity (HD, COPD). Conclusion: The concurrence of COPD and CHD is more unfavorable for the development and severity of endothelial dysfunction, which may lead to mutual aggravation syndrome, the rapider progression of the diseases, and the increased frequency of complications.

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