Asthagiri Herbal Research Foundation

Perungudi, India

Asthagiri Herbal Research Foundation

Perungudi, India
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Ponnusamy K.,Entomology Research Institute | Ponnusamy K.,Konkuk University | Petchiammal C.,SRM University | Mohankumar R.,Asthagiri Herbal Research Foundation | Hopper W.,SRM University
Journal of Ethnopharmacology | Year: 2010

Ethnopharmacological relevance: Acalypha indica, Cassia alata, Lawsonia inermis, Punica granatum, Thespesia populnea and Wrightia tinctoria are folklore medicines extensively used in the treatment of ringworm infections and skin related diseases in Tamil Nadu, India. Aim: The present study was designed to investigate the in vitro antifungal activity of certain medicinal plants and the pure compound indirubin isolated from Wrightia tinctoria. Materials and methods: The hexane, chloroform, methanol and ethanol extracts of six different plants were investigated against dermatophytes, non-dermatophytes and yeasts. Chloroform extract of Wrightia tinctoria leaf was fractionated using column chromatography and the major compound was identified using spectroscopic techniques. Antifungal activity was studied by spore germination test using agar dilution method. The minimum inhibitory concentration (MIC) was determined using broth microdilution method. Results: Wrightia tinctoria showed promising activity against dermatophytic and non-dermatophytic fungi. Leaf chloroform extract showed activity at 0.5. mg/ml against Trichophyton rubrum, Epidermophyton floccosum, Aspergillus niger and Scopulariopsis brevicaulis. The major compound, identified as indirubin, exhibited activity against dermatophytes such as Epidermophyton floccosum (MIC = 6.25 μg/ml); Trichophyton rubrum and Trichophyton tonsurans (MIC = 25 μg/ml); Trichophyton mentagrophytes and Trichophyton simii (MIC = 50 μg/ml). It was also active against non-dermatophytes (Aspergillus niger, Candida albicans and Cryptococcus sp.) within a MIC range of 0.75-25 μg/ml. Conclusion: The indole compound indirubin from Wrightia tinctoria showed antifungal activity and may be useful in the treatment of dermatophytosis. © 2010 Elsevier Ireland Ltd.

Gopalakrishnan G.,Schering | Narasimhan S.,Asthagiri Herbal Research foundation
International Journal of Drug Development and Research | Year: 2012

From the 6 Indian medicinal plants belongs to 3 botanical families were selected on the basis of their use in the treatment of infectious diseases. Hexane and ethanol extracts of the plants were studied for their antimicrobial activity against various human pathogenic organism against Gram -positive bacteria S. aureus, S. epidermitis, B. subtilis, B. cereus, M. luteus, and Gram -negative E. coli, P. aeruginosa and fungi C. albicans, A. niger was studied by disc diffusion method. The extracts of S. fruticosa, T.asiatica, T. ciliata showed marked antimicrobial activity. It is significant to note that though the plant extracts showed activity against P. aeruginosa they had no activity against the other Gram - negative bacteria E. coli. Concentration tested was 5 μl of the plant extracts (250 μg /disc) in each sterile filter paper disc (diameter, 6 mm). © 2012 IJDDR, P. Malairajan et al.

Rajesh R.P.,Suganthi Devadason Marine Research Institute | Rajesh R.P.,Indian Institute of Science | Ramasamy M.S.,Asthagiri Herbal Research Foundation | Murugan A.,Suganthi Devadason Marine Research Institute
Medicinal Chemistry | Year: 2010

The cytotoxicity-based chemotherapy treatment for cancer has significant side effects. Therefore, anticancer research is concentrated on identifying an effective drug with minimal side effects. The methanol extract of ascidian Polyclinum indicum and its column fraction PI-8 showed cytotoxicity against cervical cancer cells (HeLa) with an IC50 of 77.5 μg/mL and 1.12 μg/mL. Hoechst staining with PI-8 treated cells portrayed the apoptotic events in drug-induced apoptosis. The PI-8 markedly arrested G2/M and S phases in cell cycle. The occurrence of a second population of apoptotic cells in the range of lower Forward Scatter (FSC) and higher Side Scatter (SSC) compared to control cells indicated the characteristic feature of the cells dying of apoptosis. DNA fragmentation has established the apoptotic event. The study emphasized the fraction PI-8 of P. indicum as a potential lead compound for apoptosis targeted anticancer drugs. The molecular mechanism and chemotherapeutic potential of the fraction PI-8 need further investigation. © 2010 Bentham Science Publishers Ltd.

Ramasamy M.S.,Asthagiri Herbal Research Foundation | Manikandan S.,Madurai Kamaraj University
Mini-Reviews in Medicinal Chemistry | Year: 2011

The oceans are a source of combinatorial library of unique natural products, 'not found in the terrestrial environment'. Marine invertebrates such as sponges, molluscs, bryozoans, tunicates (Urochordata) and their associated microorganisms are the major representatives of promising bioactive compounds. Among these, the predatory molluscan cone snails have evolved with highly structured small and complex array of peptides (more than 50,000) linked to their prey capture and defence. These peptides have become a valuable source of neuro pharmacological targets as many of them selectively modulate ion channels and transporters. A group of scientists from United States, Europe, Australia, Israel and China has characterized drugs for neuropathic pain and pharmacological targets from the peptides of a few cone snail species. Several are now in clinical and preclinical development. Less than 1% of the cono peptides are pharmacologically characterized. India has a diversity of 20-30% of total cone snail species distributed worldwide. A group of Indian Scientists has made promising drug discovery programs from Conus peptides. This review will focus on the Conus peptides from Indian cone snails species, their neuro pharmacological targets and future directions. © 2011 Bentham Science Publishers Ltd.

Balachari M.,Asthagiri Herbal Research Foundation | Dhivya S.,Perungudi Industrial Estate | Srinivasan N.,Asthagiri Herbal Research Foundation
Journal of Chemical and Pharmaceutical Research | Year: 2012

Six potential Bi and Tridentate chiral molecules were synthesized and characterized, in that the amino ester and 2-amino-3,5-dibromobenzaldhehyde were linked by C=N bond which can be specifically reduced using Tetrabutylammonium borohydride and ester further convert into Alcohol using lithium borohydride. The synthesized compounds are evaluated for molecular docking to check potency of the synthesize compounds for HIV-protease.

PubMed | University of Madras and Asthagiri Herbal Research Foundation
Type: Journal Article | Journal: Acta crystallographica. Section E, Crystallographic communications | Year: 2017

In the title compounds, C

Ponnusamy K.,Entomology Research Institute | Ramasamy M.,Asthagiri Herbal Research Foundation | Savarimuthu I.,Entomology Research Institute | Paulraj M.G.,Entomology Research Institute
Scandinavian Journal of Infectious Diseases | Year: 2010

Staphylococcus aureus is a common multidrug-resistant organism in hospital-acquired infections, and the NorA efflux pump mechanism facilitates resistance to quinone compounds. In India, Wrightia tinctoria R. Br. leaves have traditionally been used to treat skin diseases and have been explored for antibacterial and efflux pump inhibition (EPI) compounds. In this study, indirubin isolated from the chloroform extract of W. tinctoria R. Br. leaves was tested for its antibacterial activity against clinically important Gram-positive and Gram-negative bacteria and the minimum inhibitory concentration (MIC) was determined using broth microdilution. The EPI properties of indirubin were investigated using Staphylococcus aureus SA1199B, and its synergistic effects were tested with ciprofloxacin. Indirubin showed antibacterial activity against both the type strain and drug-resistant S. aureus; the MIC was determined to be 12.5 mg/l for S. aureus and 25 mg/l for Staphylococcus epidermidis. However, it synergistically (fractional inhibitory concentration index 0.45) potentiated the activity of ciprofloxacin, probably by inhibiting the NorA efflux pump. Indirubin exhibited EPI activity nearly comparable to that of reserpine by 4-fold reduction in ciprofloxacin MIC. Our results suggest that the natural compound indirubin could be used in future therapeutic applications as a potential EPI. © 2010 Informa UK Ltd.

Ilango K.,Tamil University | Maharajan G.,Chettinad Hospital and Research Institute | Narasimhan S.,Asthagiri Herbal Research Foundation
Natural Product Research | Year: 2013

This study was carried out to evaluate the anti-nociceptive and anti-inflammatory activities of carbon tetrachloride extract (CTCE) of Azadirachta indica fruit skin and its isolated constituent azadiradione at two different dose levels (50 and 100mg kg-1 body weight). Anti-nociceptive screening by writhing test and hotplate technique supported both peripheral and central mechanisms, respectively. Anti-inflammatory activity was observed using carrageenan-induced paw oedema model. The results concluded that the animals treated with 100mg kg-1 dose of CTCE and azadiradione exhibited significant anti-nociceptive and anti-inflammatory activities. This study had rationalised the ethnomedicinal use of the plant for wound, burns and injury by tribal people. © 2013 Taylor & Francis.

Murugan A.,Suganthi Devadason Marine Research Institute | Begum M.S.,Suganthi Devadason Marine Research Institute | Ramasamy M.S.,Asthagiri Herbal Research Foundation | Raja P.,Suganthi Devadason Marine Research Institute
Natural Product Research | Year: 2012

The seaweeds Dictyota dichotoma and Chaetomorpha linoides from the southeast coast of India were screened for anti-microfouling activity against biofilm bacteria, anti-macrofouling activity against brown mussels and feeding deterrence activity against the sea angel Monodactylus kottelati. The surface associated epiphytic bacteria were also isolated from seaweeds and screened for activity against biofilm bacteria. The acetone extracts showed a wide spectrum activity against biofilm bacteria and the algal metabolite was surface concentrated and non-polar in nature. The seaweeds also inhibited byssus production and attachment in brown mussels, and deterred feeding in the sea angel. The lower epiphytic bacterial number on the seaweed's surface compared to the surrounding seawater medium indicated selective inhibition or surface mediation. The epiphytic bacteria, which showed activity against biofilm bacteria, might also possibly play a role in seaweed defence strategies. The 50% deterrence of feeding activity at lower concentrations was not proportionate to the 100% inhibition concentration, which could be attributed to the adaptability of the fishes, an indication that the active substances are inhibitory in nature. This was further substantiated with the 100% recovery of mussels in a toxicity assay and the lower EC 50 values than LC 50 values in the mussel bioassay. The study indicates that the metabolites of both seaweeds have ecological significance and could possibly play a multifunctional role. © 2012 Taylor & Francis.

Ramya K.,ARC International | Dhathathreyan K.S.,ARC International | Sreenivas J.,ARC International | Kumar S.,Asthagiri Herbal Research Foundation | Narasimhan S.,Asthagiri Herbal Research Foundation
International Journal of Energy Research | Year: 2013

Alcoholysis of sodium borohydride offers advantages due to reduction in number of steps for recycling of sodium borohydride, elimination of freezing problems that are associated with the use of water, fast generation of hydrogen, etc. Methanol was used to liberate hydrogen from sodium borohydride. The influence of the amount of solvent, substrate concentration, temperature and catalyst on the kinetics of alcoholysis reaction in non-stabilized sodium borohydride has been examined in the present study. The reaction was found to be first order with respect to substrate concentration and zero order with respect to solvent concentration. Effect of soluble metal salts, metal powder and metal boride as catalysts on hydrogen generation rate has also been investigated. It was found that NiCl2, Ni2B and RuCl3 were effective catalysts and hydrogen generation proceeds with high efficiency in the presence of these catalysts. © 2013 John Wiley & Sons, Ltd.

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