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PubMed | University of Madras and Asthagiri Herbal Research Foundation
Type: Journal Article | Journal: Acta crystallographica. Section E, Crystallographic communications | Year: 2017

In the title compounds, C


PubMed | Anna University, University of Madras and Asthagiri Herbal Research Foundation
Type: Journal Article | Journal: Journal of biomolecular structure & dynamics | Year: 2016

4-[(1Z)-1-(2-carbamothioylhydrazinylidene)ethyl]phenyl acetate [Ace semi],4-[(1Z)-1-(2-carbamothioylhydrazinylidene)ethyl]phenyl propanoate [Pro semi] from the family of thiosemicarbazones derivative has been newly synthesized. It has good anticancer activity as well as antibacterial and it is also less toxic in nature, its binding characteristics are therefore of huge interest for understanding pharmacokinetic mechanism of the drug. The binding of thiosemicarbazone derivative to human serum albumin (HSA) has been investigated by studying its quenching mechanism, binding kinetics and the molecular distance (r) between donor (HSA) and acceptor (thiosemicarbazone derivative) was estimated according to Forsters theory of non-radiative energy transfer using fluorescence spectroscopy. The binding dynamics has been elaborated using synchronous fluorescence spectroscopy, and the feature of thiosemicarbazone derivative induced structural changes of HSA has been studied by circular dichorism, Fourier transform infrared spectroscopy. Molecular modelling simulations explore the hydrophobic interaction and hydrogen bonding which stabilizes the interaction.


PubMed | University of Madras and Asthagiri Herbal Research Foundation
Type: Journal Article | Journal: Acta crystallographica. Section E, Crystallographic communications | Year: 2015

The asymmetric unit of the title compound, C17H17N3O2S, consists of two independent mol-ecules, A and B, with different conformations: in mol-ecule A, the dihedral angles between the central benzene ring and the pendant tolyl and carbamo-thio-ylhydrazono groups are 71.12(9) and 5.95(8), respectively. The corresponding angles in mol-ecule B are 50.56(12) and 26.43(11), respectively. Both mol-ecules feature an intra-molecular N-HN hydrogen bond, which closes an S(5) ring. In the crystal, mol-ecules are linked by N-HO, N-HS and C-HO hydrogen bonds, generating a three-dimensional network.


PubMed | Anna University, University of Madras and Asthagiri Herbal Research Foundation
Type: | Journal: Journal of biomolecular structure & dynamics | Year: 2016

4-[3-acetyl-5-(acetylamino)-2,3-dihydro-1,3,4-thiadiazole-2-yl]phenyl benzoate from the family of thiadiazole derivative has been newly synthesized. It has good anticancer activity as well as antibacterial and less toxic in nature, its binding characteristics are therefore of huge interest for understanding pharmacokinetic mechanism of the drug. The binding of thiadiazole derivative to human serum albumin (HSA) has been investigated by studying its quenching mechanism, binding kinetics and the molecular distance, r between the donor (HSA) and acceptor (thiadiazole derivative) was estimated according to Forsters theory of non-radiative energy transfer. The Gibbs free energy (G), enthalpy (H) and entropy (S) changes of temperature-dependent K


PubMed | Madha Engineering College, National Institute of Technology Tiruchirappalli and Asthagiri Herbal Research Foundation
Type: | Journal: BioMed research international | Year: 2015

A bacterial strain was isolated from tannery effluent which can tolerate high concentrations of potassium dichromate up to 1000ppm. The isolated microorganism was identified as Pseudomonas aeruginosa by performing biochemical tests and molecular characterization. In the presence of excess of carbohydrate source, which is a physiological stress, this strain produces Polyhydroxybutyrate (PHB). This intracellular polymer, which is synthesized, is primarily a product of carbon assimilation and is employed by microorganisms as an energy storage molecule to be metabolized when other common energy sources are limitedly available. Efforts were taken to check whether the PHB has any positive effect on spent wash decolorization. When a combination of PHB and the isolated bacterial culture was added to spent wash, a maximum color removal of 92.77% was found which was comparatively higher than the color removed when the spent wash was treated individually with the PHB and Pseudomonas aeruginosa. PHB behaved as a support material for the bacteria to bind to it and thus develops biofilm, which is one of the natural physiological growth forms of microorganisms. The bacterial growth in the biofilm and the polymer together acted in synergy, adsorbing and coagulating the pollutants in the form of color pigments.


Kumar S.,Asthagiri Herbal Research Foundation | Priya Matharasi D.,Asthagiri Herbal Research Foundation | Gopi S.,Asthagiri Herbal Research Foundation | Sivakumar S.,Asthagiri Herbal Research Foundation | Narasimhan S.,Asthagiri Herbal Research Foundation
Journal of Asian Natural Products Research | Year: 2010

Aloin (10-glucopyranosyl-1,8-dihydroxy-3-hydroxymethyl-9(10H)-anthracenone) , a bioactive compound in Aloe vera, although known to have an anticancer effect, has not been used in current drug research. Optimization of the lead structure could enhance the utility of this compound. Hence, aloin was modified using natural amino acids to produce Schiff's base, a potential pharmacophore, and its corresponding aglycones. The synthetic derivatives exhibited significant enhancement in their efficacy toward antioxidant (DPPH radical scavenging) and cytotoxic activities than those of the parent compound, aloin showing promise for application in cancer treatment. © 2010 Taylor & Francis.


Ponnusamy K.,Entomology Research Institute | Ramasamy M.,Asthagiri Herbal Research Foundation | Savarimuthu I.,Entomology Research Institute | Paulraj M.G.,Entomology Research Institute
Scandinavian Journal of Infectious Diseases | Year: 2010

Staphylococcus aureus is a common multidrug-resistant organism in hospital-acquired infections, and the NorA efflux pump mechanism facilitates resistance to quinone compounds. In India, Wrightia tinctoria R. Br. leaves have traditionally been used to treat skin diseases and have been explored for antibacterial and efflux pump inhibition (EPI) compounds. In this study, indirubin isolated from the chloroform extract of W. tinctoria R. Br. leaves was tested for its antibacterial activity against clinically important Gram-positive and Gram-negative bacteria and the minimum inhibitory concentration (MIC) was determined using broth microdilution. The EPI properties of indirubin were investigated using Staphylococcus aureus SA1199B, and its synergistic effects were tested with ciprofloxacin. Indirubin showed antibacterial activity against both the type strain and drug-resistant S. aureus; the MIC was determined to be 12.5 mg/l for S. aureus and 25 mg/l for Staphylococcus epidermidis. However, it synergistically (fractional inhibitory concentration index 0.45) potentiated the activity of ciprofloxacin, probably by inhibiting the NorA efflux pump. Indirubin exhibited EPI activity nearly comparable to that of reserpine by 4-fold reduction in ciprofloxacin MIC. Our results suggest that the natural compound indirubin could be used in future therapeutic applications as a potential EPI. © 2010 Informa UK Ltd.


Ilango K.,Tamil University | Maharajan G.,Chettinad Hospital and Research Institute | Narasimhan S.,Asthagiri Herbal Research Foundation
Natural Product Research | Year: 2013

This study was carried out to evaluate the anti-nociceptive and anti-inflammatory activities of carbon tetrachloride extract (CTCE) of Azadirachta indica fruit skin and its isolated constituent azadiradione at two different dose levels (50 and 100mg kg-1 body weight). Anti-nociceptive screening by writhing test and hotplate technique supported both peripheral and central mechanisms, respectively. Anti-inflammatory activity was observed using carrageenan-induced paw oedema model. The results concluded that the animals treated with 100mg kg-1 dose of CTCE and azadiradione exhibited significant anti-nociceptive and anti-inflammatory activities. This study had rationalised the ethnomedicinal use of the plant for wound, burns and injury by tribal people. © 2013 Taylor & Francis.


Murugan A.,Suganthi Devadason Marine Research Institute | Begum M.S.,Suganthi Devadason Marine Research Institute | Ramasamy M.S.,Asthagiri Herbal Research Foundation | Raja P.,Suganthi Devadason Marine Research Institute
Natural Product Research | Year: 2012

The seaweeds Dictyota dichotoma and Chaetomorpha linoides from the southeast coast of India were screened for anti-microfouling activity against biofilm bacteria, anti-macrofouling activity against brown mussels and feeding deterrence activity against the sea angel Monodactylus kottelati. The surface associated epiphytic bacteria were also isolated from seaweeds and screened for activity against biofilm bacteria. The acetone extracts showed a wide spectrum activity against biofilm bacteria and the algal metabolite was surface concentrated and non-polar in nature. The seaweeds also inhibited byssus production and attachment in brown mussels, and deterred feeding in the sea angel. The lower epiphytic bacterial number on the seaweed's surface compared to the surrounding seawater medium indicated selective inhibition or surface mediation. The epiphytic bacteria, which showed activity against biofilm bacteria, might also possibly play a role in seaweed defence strategies. The 50% deterrence of feeding activity at lower concentrations was not proportionate to the 100% inhibition concentration, which could be attributed to the adaptability of the fishes, an indication that the active substances are inhibitory in nature. This was further substantiated with the 100% recovery of mussels in a toxicity assay and the lower EC 50 values than LC 50 values in the mussel bioassay. The study indicates that the metabolites of both seaweeds have ecological significance and could possibly play a multifunctional role. © 2012 Taylor & Francis.


Ramya K.,ARC International | Dhathathreyan K.S.,ARC International | Sreenivas J.,ARC International | Kumar S.,Asthagiri Herbal Research Foundation | Narasimhan S.,Asthagiri Herbal Research Foundation
International Journal of Energy Research | Year: 2013

Alcoholysis of sodium borohydride offers advantages due to reduction in number of steps for recycling of sodium borohydride, elimination of freezing problems that are associated with the use of water, fast generation of hydrogen, etc. Methanol was used to liberate hydrogen from sodium borohydride. The influence of the amount of solvent, substrate concentration, temperature and catalyst on the kinetics of alcoholysis reaction in non-stabilized sodium borohydride has been examined in the present study. The reaction was found to be first order with respect to substrate concentration and zero order with respect to solvent concentration. Effect of soluble metal salts, metal powder and metal boride as catalysts on hydrogen generation rate has also been investigated. It was found that NiCl2, Ni2B and RuCl3 were effective catalysts and hydrogen generation proceeds with high efficiency in the presence of these catalysts. © 2013 John Wiley & Sons, Ltd.

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