Yamamori K.,Astellas Pharma Tec Co. |
Watanabe T.,Astellas Pharma Tec Co. |
Inada M.,Astellas Pharma Tec Co. |
Wakayama T.,Astellas Pharma Tec Co. |
And 3 more authors.
Bunseki Kagaku | Year: 2013
A high-speed HPLC analysis for fermentation product A in the purification process was evaluated by using four kinds of commercially available core shell columns (75×4.6 mm i.d.; particle size, 2.6~2.7 μm) instead of a general porous particle column (Honenpak column, 75×4.6 mm i.d.; particle size, 3 μm). These core shell columns (Ascentis®Express C18, HALO C18, SunShell C18, Kinetex) showed similar performances to the porous particle column (Honenpack) in terms of the theoretical plate, asymmetry factor and pump pressure under almost the same operation conditions. Especially, SunShell C18 exhibited the highest theoretical plate among them, which made it possible to shorten the analysis time (the retention time of compound A was 6.6 minutes for SunShell C18 and 14.6 minutes for Honenpack, respectively). Our results confirmed that core shell columns are available to reduce the total analysis time for process tests that usually require a rapid response to proceed the process. © 2013 The Japan Society for Analytical Chemistry.