Associazione Nazionale Allevatori Specie Bufalina ANASB

Caserta, Italy

Associazione Nazionale Allevatori Specie Bufalina ANASB

Caserta, Italy
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Bonfatti V.,University of Padua | Giantin M.,University of Padua | Gervaso M.,University of Padua | Coletta A.,Associazione Nazionale Allevatori Specie Bufalina ANASB | And 2 more authors.
Journal of Dairy Science | Year: 2012

The aim of this study was to estimate effects of CSN1S1-CSN3 (αS1-κ-casein) composite genotypes on milk production traits and milk coagulation properties (MCP) in Mediterranean water buffalo. Genotypes at CSN1S1 and CSN3 and coagulation properties [rennet clotting time (RCT), curd firming time (K20), and curd firmness (A30)] were assessed by reversed-phase HPLC and computerized renneting meter analysis, respectively, using single test-day milk samples of 536 animals. Alternative protein variants of αS1-CN and κ-CN were detected by HPLC, and identification of the corresponding genetic variants was carried out by DNA analysis. Two genetic variants were detected at CSN1S1 (A and B variants) and 2 at CSN3 (X1 and X2 variants). Statistical inference was based on a linear model including the CSN1S1-CSN3 composite genotype effect (7 genotypes), the effects of herd-test-day (8 levels), and a combined days in milk (DIM)-parity class. Composite genotype AB-X2X2 was associated with decreased test-day milk yield [-0.21 standard deviation (SD) units of the trait] relative to genotype BB-X2X2. Genotypes did not affect milk protein content, but genotype AB-X1X1 was associated with increased fat content compared with genotype BB-X2X2 (+0.28 SD units of the trait) and AB-X1X1 (+0.43 SD units of the trait). For RCT, the largest difference (+1.91min; i.e., 0.61 SD units of the trait) was observed between genotype AA-X1X2 and AB-X1X1. Direction of genotype effects on K20 was consistent with that for RCT. The maximum variation in K20 due to genotype effects (between AA-X1X2 and AB-X1X1 genotypes) was almost 0.9 SD units of the trait. Magnitude of genotype effects was smaller for A30 than for RCT and K20, with a maximum difference of 0.5 SD units of the trait between genotype AA-X1X2 and AA-X1X1. The B allele at CSN1S1 was associated with increased RCT and K20 and with weaker curds compared with allele A. Allele X2 at CSN3 exerted opposite effects on MCP relative to CSN1S1 B. Because of linkage disequilibrium, allele B at CSN1S1 and allele X2 at CSN3 tend to be associated and this likely makes their effects cancel each other. This study indicates a role for casein genes in variation of MCP of buffalo milk. Further studies are necessary to estimate the effects of casein genetic variants on variation of cheese yield. © 2012 American Dairy Science Association.


Bonfatti V.,University of Padua | Gervaso M.,University of Padua | Rostellato R.,University of Padua | Coletta A.,Associazione Nazionale Allevatori Specie Bufalina ANASB | Carnier P.,University of Padua
Journal of Dairy Science | Year: 2013

The aim of this study was to investigate the effects exerted by the content of casein and whey protein fractions on variation of pH, rennet-coagulation time (RCT), curd-firming time (K20), and curd firmness of Mediterranean buffalo individual milk. Measures of milk protein composition and assessment of genotypes at CSN1S1 and CSN3 were obtained by reversed-phase HPLC analysis of 621 individual milk samples. Increased content of αS1-casein (CN) was associated with delayed coagulation onset and increased K20, whereas average pH, RCT, and K20 decreased when β-CN content increased. Milk with low κ-CN content exhibited low pH and RCT relative to milk with high content of κ-CN. Increased content of glycosylated κ-CN was associated with unfavorable effects on RCT. Effects of milk protein composition on curd firmness were less important than those on pH, RCT, and K20. Likely, this occurred as a consequence of the very short RCT of buffalo milk, which guaranteed a complete strengthening of the curd even in the restricted 31 min time of analysis of coagulation properties and for samples initially showing soft curds. Effects of CSN1S1-CSN3 genotypes on coagulation properties were not to be entirely ascribed to existing variation in milk protein composition associated with polymorphisms at CSN1S1 and CSN3 genes. Although the role of detailed milk protein composition in variation of cheese yield needs to be further investigated, findings of this study suggest that modification of the relative content of specific CN fractions can relevantly influence the behavior of buffalo milk during processing. © 2013 American Dairy Science Association.


Bonfatti V.,University of Padua | Giantin M.,University of Padua | Gervaso M.,University of Padua | Rostellato R.,University of Padua | And 3 more authors.
Journal of Dairy Science | Year: 2012

The aim of the study was to investigate the effect of composite CSN1S1-CSN3 [αS1-κ-casein (CN)] genotype on milk protein composition in Mediterranean water buffalo. Content of αS1-CN, αS2-CN, β-CN, γ-CN, κ-CN, glycosylated and unglycosylated κ-CN, α-lactalbumin, and β-lactoglobulin was measured by reversed-phase HPLC using 621 individual milk samples. Genotypes at CSN1S1 and CSN3 were also obtained by reversed-phase HPLC. Two alleles were detected at CSN1S1 (corresponding to the A and B variants, O62823: p.Leu193Ser,) and at CSN3 (corresponding to the X1 and X2 variants, CAP12622.1: p.Ile156Thr). Increased proportions of αS1-CN in total casein (TCN) were associated with genotypes carrying CSN1S1 A. Genotypes associated with a marked decrease of the proportion of αS1-CN in TCN (composite genotypes AB-X1X1 and BB-X1X2) were associated with marked increases in the proportion of αS2-CN. In addition, composite genotypes carrying the X1 allele at CSN3 were associated with a greater proportion of αS2-CN in TCN relative to those carrying CSN3 X2. Composite genotypes greatly affected also the variability of ratios of κ-CN to TCN, with genotypes carrying the X1 allele at CSN3 being associated with decreased ratios. The decreased content of glycosylated κ-CN associated with CSN3 X1 was responsible for the overall lower content of total κ-CN in milk of X1-carrying animals. Increasing the frequency of specific genotypes might be an effective way to alter milk protein composition, namely the proportion of αS1-CN, αS2-CN, and κ-CN in TCN, and the degree of glycosylation of κ-CN. © 2012 American Dairy Science Association.


Serafini R.,University of Naples Federico II | Love C.C.,Texas A&M University | Coletta A.,Associazione Nazionale Allevatori Specie Bufalina ANASB | Mari G.,University of Bologna | And 3 more authors.
Animal Reproduction Science | Year: 2016

The relationship among sperm attributes of DNA integrity, sperm motility, morphology, viability, acrosome integrity and in vivo fertility of frozen-thawed Italian Mediterranean Buffalo (IMB) sperm has not been reported. Straws of frozen-thawed semen samples from three bulls were examined. Sperm DNA assays (i.e., neutral Comet assay, Sperm Bos Halomax-SBH and Sperm Chromatin Structure Assay-SCSA) were not correlated to each other (P > 0.05). Many neutral Comet assay measures were correlated to total sperm motility-TMOT (% head-H-DNA, r = 0.74; Olive moment, r = −0.76; P < 0.05) and coiled tails (r-values ranged from% H-DNA, r = −0.80 to tail length, r = −0.71; P < 0.05). The COMP-αt was negatively correlated to viable acrosome intact (VAI) sperm, and distal droplets (r = −0.60 and −0.61; P < 0.05), whereas Mean-αt and Mode-αt were positively correlated to bent midpieces (r = 0.63 and 0.61; P < 0.05). The SBH assay was positively correlated to non-viable acrosome damaged (NVAD) sperm (r = 0.60; P < 0.05) and negatively correlated to viable acrosome damaged (VAD) sperm (r = −0.63; P < 0.05). The overall pregnancy rate (PR-at 30 and 45 d post artificial insemination-AI) and the calving rate were 57%, 55% and 45%, respectively. Among sperm features analyzed the area under the Receiver Operating Characteristic (ROC) Curve was significant (P < 0.05) for TMOT, NVAD, Standard Deviation-αt (SD-αt) and neutral comet measures (Olive tail moment and tail moment, % H- DNA and tail area) in estimating pregnancy. © 2016 Elsevier B.V.


Cosenza G.,University of Naples Federico II | Pauciullo A.,University of Naples Federico II | Coletta A.,Associazione Nazionale Allevatori Specie Bufalina ANASB | Di Francia A.,University of Naples Federico II | And 4 more authors.
Journal of Dairy Science | Year: 2011

Buffalo milk is characterized by the presence of all 4 casein fractions (α S1, β, α S2, and κ) encoded by the 4 tightly linked autosomal genes (CSN1S1, CSN2, CSN1S2, and CSN3, respectively). In the present paper, we report for the first time a quantitative characterization of buffalo casein transcripts and show that the 4 genes are not transcribed and translated with the same efficiency. In particular, the analysis of individual milk samples obtained from 9 Mediterranean river buffaloes showed that the most abundant casein fractions were β (53.45%) and α S1 (20.61%), followed by α S2 and κ, at 14.28 and 11.66%, respectively. Quantification of the corresponding mRNA showed that the percentage of transcripts of the 4 caseins was 16.48, 23.18, 55.87, and 4.47% for α S1, β, α S2, and κ, respectively. Translation efficiency was 0.25 for CSN1S2, 1.31 for CSN1S1, 2.39 for CSN2, and 2.69 for the CSN3 transcripts, respectively. A comparison of nucleotide sequences with the Kozak consensus sequence was also carried out to investigate if the mRNA sequences might be responsible for the observed differences. © 2011 American Dairy Science Association.


PubMed | Associazione Nazionale Allevatori Specie Bufalina ANASB, University of Bologna, University of Naples Federico II and Texas A&M University
Type: | Journal: Animal reproduction science | Year: 2016

The relationship among sperm attributes of DNA integrity, sperm motility, morphology, viability, acrosome integrity and in vivo fertility of frozen-thawed Italian Mediterranean Buffalo (IMB) sperm has not been reported. Straws of frozen-thawed semen samples from three bulls were examined. Sperm DNA assays (i.e., neutral Comet assay, Sperm Bos Halomax-SBH and Sperm Chromatin Structure Assay-SCSA) were not correlated to each other (P>0.05). Many neutral Comet assay measures were correlated to total sperm motility-TMOT (% head-H-DNA, r=0.74; Olive moment, r=-0.76; P<0.05) and coiled tails (r-values ranged from% H-DNA, r=-0.80 to tail length, r=-0.71; P<0.05). The COMP-t was negatively correlated to viable acrosome intact (VAI) sperm, and distal droplets (r=-0.60 and -0.61; P<0.05), whereas Mean-t and Mode-t were positively correlated to bent midpieces (r=0.63 and 0.61; P<0.05). The SBH assay was positively correlated to non-viable acrosome damaged (NVAD) sperm (r=0.60; P<0.05) and negatively correlated to viable acrosome damaged (VAD) sperm (r=-0.63; P<0.05). The overall pregnancy rate (PR-at 30 and 45d post artificial insemination-AI) and the calving rate were 57%, 55% and 45%, respectively. Among sperm features analyzed the area under the Receiver Operating Characteristic (ROC) Curve was significant (P<0.05) for TMOT, NVAD, Standard Deviation-t (SD-t) and neutral comet measures (Olive tail moment and tail moment, % H- DNA and tail area) in estimating pregnancy.

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