Associazione Isola dei Centenari

Sassari, Italy

Associazione Isola dei Centenari

Sassari, Italy
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Pasella S.,University of Sassari | Baralla A.,University of Sassari | Canu E.,University of Sassari | Pinna S.,University of Sassari | And 9 more authors.
Proteome Science | Year: 2013

Background: This study examined the effect of storage temperature on the protein profile of human plasma. Plasma samples were stored for 13 days at -80°C, -20°C, +4°C and room temperature (20-25°C) prior to proteomic analysis. The proteomic comparisons were based on the differences of mean intensity values of protein spots between fresh plasma samples (named " time zero" ) and plasma samples stored at different temperatures. To better understand the thermally induced biochemical changes that may affect plasma proteins during storage we identified proteins with different expressions with respect to the time zero sample.Results: Using two-dimensional electrophoresis followed by MALDI-TOF MS and /or LC-MS/MS 20 protein spots representing 10 proteins were identified with significant differences in abundance when stored at different temperatures. Our results, in agreement with various authors, indicate that during storage for a short period (13 days) at four different temperatures plasma proteins were more affected by degradation processes at +4°C compared to the other temperatures analysed. However, we founded that numerous protein spots (vitamin D binding protein, alpha-1-antitrypsin, serotransferrin, apoplipoprotein A-I, apolipoprotein E, haptoglobin and complement factor B) decrease in abundance with increasing temperature up to 4°C, but at room temperature their intensity mean values are similar to those of time zero and -80°C. We hypothesize that these proteins are labile at 4°C, but at the same time they are stable at room temperature (20-25°C). Furthermore we have grouped the proteins based on their different sensitivity to the storage temperature. Spots of serum albumin, fibrinogen gamma chain and haptoglobin are more resistant to the higher temperatures tested, as they have undergone changes in abundance only at room temperature; conversely, other spots of serum albumin, fibrinogen beta chain and serotransferrin are more labile as they have undergone changes in abundance at all temperatures except at -80°C.Conclusions: Although there are many studies concerning protein stability of clinical samples during storage these findings may help to provide a better understanding of the changes of proteins induced by storage temperature. © 2013 Pasella et al.; licensee BioMed Central Ltd.

Ventura V.,University of Sassari | Posadinu M.,Dermatologia ASL 1 | Deiana M.,Associazione Isola dei Centenari | Baralla A.,University of Sassari | And 8 more authors.
Biochimica Clinica | Year: 2015

Bullous pemhigoid (BP) is an autoimmune subepidermal blistering disease, usually occurring in the elderly. In Europe the incidence of BP has doubled in the last 10 years. The disease has a chronic relapsing course and a relatively benign prognosis. An increasing number of publications report altered expression of a number of cytokines. However, at the present it is not possible to establish a specific role for them in the disease. We report a case of a centenarian woman with a BP occurrence in otherwise optimal health conditions. Besides the usual biochemical investigations, plasma concentrations of a number of cytokines were evaluated during the disease course (relapse and remission). The majority of the measured cytokines were elevated in the active phase of the disease and returned to values similar to those presented by a group of apparently healthy subjects of similar age during the remission. This case could be useful to better understand the role of cytokines in autoimmune skin diseases of the aging. © 2014 Biochimica Clinica.

Forte G.,Italian National Institute for Health | Deiana M.,Associazione Isola dei Centenari | Pasella S.,University of Sassari | Baralla A.,University of Sassari | And 10 more authors.
Experimental Gerontology | Year: 2014

The concentration of calcium (Ca), cobalt (Co), copper (Cu), iron (Fe), magnesium (Mg), manganese (Mn) and selenium (Se) in plasma of 76 nonagenarians (mean age, 89.0. ±. 6.3. years), 64 centenarians (mean age, 101. ±. 1. years) and 24 middle-aged subjects as controls (mean age 61.2. ±. 1.1. years), was determined by sector field inductively coupled plasma mass spectrometry. All the subjects lived in Sardinia, an Italian island, that has the higher prevalence of centenarians than in other European countries. A comparison among the three classes of age showed a significant depletion of Ca, Co, Fe, Mn and Se (all p. <. 0.001) in nonagenarians and centenarians with respect to controls. In particular, the geometric mean (GM) values of Ca, Co, Fe, Mn and Se were: 94.1. μg/ml, 0.46. ng/ml, 1314. ng/ml, 2.47. ng/ml and 111. ng/ml in controls; 87.6. μg/ml, 0.22. ng/ml, 815. ng/ml, 1.07. ng/ml and 88.9. ng/ml in nonagenarians; 87.0. μg/ml, 0.29. ng/ml, 713. ng/ml, 1.27. ng/ml and 81.9. ng/ml in centenarians. The highest inverse relationship with age was observed for Fe (p. <. 0.001; ρ. = - 0.352) and Se (p. <. 0.001; ρ. = - 0.417). This trend was also observed when data were sorted by gender. On the other hand, Cu and Mg levels in plasma remained substantially unchanged during aging. As regards Cu, it was significantly higher in females than in males in controls (GM, 1294. ng/ml vs. 1077. ng/ml; p. = 0.012), in nonagenarians (GM, 1216. ng/ml vs. 1081. ng/ml; p. = 0.011) as well as in centenarians (GM, 1226. ng/ml vs. 1152. ng/ml; p. = 0.045) and in hypertensive subjects with respect to healthy people (GM, 1215. ng/ml vs. 1129. ng/ml; p. = 0.021). These data can be used to enhance knowledge and support the research on: i) metals involved in aging in areas with high rates of human longevity; ii) variables (gender, lifestyle habits and health status) as critical determinants in aging; and iii) mineral intake and supplementation at older age affecting the healthy aging. © 2014 Elsevier Inc.

Ventura V.,University of Sassari | Posadinu A.,Centro Ustioni ASL No 1 Sassari | Deiana M.,Associazione Isola Dei Centenari | Baralla A.,University of Sassari | And 8 more authors.
Biochimica Clinica | Year: 2014

TEN is a rare disease characterized by the separation of skin and mucous membranes from the derma. The reported clinical case refers to an adult female hospitalized at the Burns Unit after allopurinol prescription. During hospitalization the patient underwent the therapeutic protocol established at the Burns Unit including intravenus immunoglobulin administration and plasmapheresis. At the end of therapy a clinical improvement of both local and global conditions was observed. During the course of disease, we measured plasma concentrations of a number of cytokines. Measurements were performed using commercially available multiplex bead-based sandwich immunoassays (Biorad). We found a marked decrease in concentrations of interleukin (IL)-6, interferon β-induced protein (IP)-10 e IL-13 at various stages of apheresis therapy, particularly pronounced for IP-10. We also found a normalization of IL-6 and IP-10 concentrations during the follow-up. In addition, very high concentrations of the same cytokines in the plasma effluent after apheresis were observed. Although obtained in a single case, our results suggest a correlation between plasma concentrations of cytokines and the clinical course of TEN. It can be postulated that this approach could serve as a guide for future research in the development of targeted therapy based on the etiopathogenesis of TEN.

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