Aso Pharmaceutical Co.

Kumamoto-shi, Japan

Aso Pharmaceutical Co.

Kumamoto-shi, Japan

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Ono M.,Tokai University | Ochiai T.,Tokai University | Yasuda S.,Tokai University | Nishida Y.,Aso Pharmaceutical Co. | And 5 more authors.
Chemical and Pharmaceutical Bulletin | Year: 2013

Five new norlanostane-type triterpenoid glycosides were isolated from the bulbs of Scilla scilloides DRUCE (Liliaceae). Their chemical structures were determined on the basis of spectroscopic data as well as chemical evidence. © 2013 The Pharmaceutical Society of Japan.


Ono M.,Tokai University | Toyohisa D.,Tokai University | Morishita T.,Tokai University | Horita H.,Tokai University | And 7 more authors.
Chemical and Pharmaceutical Bulletin | Year: 2011

Three new norlanostane-type triterpene glycosides, scillanostasides A, B, and C, and two new lanostane-type triterpene glycosides, scillanostasides D and E, were isolated from the bulbs of Scilla scilloides DRUCE (Liliaceae) along with one known norlanostane-type triterpene heptaglycoside, scillascilloside G-1. Their chemical structures were determined on the basis of spectroscopic data as well as chemical evidence. © 2011 Pharmaceutical Society of Japan.


Ono M.,Tokai University | Takatsu Y.,Tokai University | Ochiai T.,Tokai University | Yasuda S.,Tokai University | And 6 more authors.
Chemical and Pharmaceutical Bulletin | Year: 2012

Two new norlanostane-type triterpenoid glycosides and a new phenylpropanoid glycoside were isolated from the bulbs of Scilla scilloides DRUCE (Liliaceae), along with two known alkaloids. Their chemical structures were determined on the basis of spectroscopic data as well as chemical evidence. © 2012 The Pharmaceutical Society of Japan.


Nishida Y.,Tokai University | Nishida Y.,Aso Pharmaceutical Co. | Sugahara S.,Tokai University | Wada K.,Tokai University | And 4 more authors.
Pharmaceutical Biology | Year: 2014

Context: Scilla scilloides Druce (Liliaceae) is a folk medicine to treat dermal inflammation; however, the medicinal properties of this plant have not been completely established.Objective: The current study investigates the potent anti-inflammatory effects of S. scilloides bulbs for its traditional usage using lipoxygenase and hyaluronidase as the inflammation model. To gain insight into the active constituents, nine homoisoflavones (1-9) were subsequently tested.Materials and methods: Lipoxygenase and hyaluronidase inhibition of ethyl acetate extract from the bulbs of this plant within 2000 μg/mL or homoisoflavones within 1000 μM were determined by colorimetric methods. RAW264.7 cells were incubated with 10 or 50 μM homoisoflavones plus lipopolysaccharide (LPS) for 24 h. The culture media were collected and analyzed for determination of the nitric oxide (NO) level by the colorimetric Griess method to measure the extent of inflammation.Results: The extract exhibited inhibitory effects on lipoxygenase and hyaluronidase activities with IC50values 31.5 and 169 μg/mL, respectively. Among the nine homoisoflavones tested, four (1 and 3-5) resulted in 79.3-97.9% higher lipoxygenase inhibition than 6.7-32.7% of the others at 500 μM. Calculated IC50values indicated 5 as the compound responsible for strong lipoxygenase inhibition with 15.8 μM as the IC50value. In the hyaluronidase assay, all homoisoflavones tested at 1000 μM demonstrated 16.2-58.0% inhibition. Incubating the cells in the presence of all nine homoisoflavones tested at 50 μM significantly suppressed the NO production, downward to 1.5-66.0%, in the LPS-activated macrophage cells as a model.Discussion and conclusion: These results may indicate a potential role of S. scilloides for anti-inflammatory purposes. © 2014 Informa Healthcare USA.


Yasuda S.,Tokai University | Nishida Y.,Tokai University | Nishida Y.,Aso Pharmaceutical Co. | Wada K.,Tokai University | And 5 more authors.
Bioscience, Biotechnology and Biochemistry | Year: 2013

The extract from bulbs of Scilla scilloides exhibited inhibitory effects in lipoxygenase and hyaluronidase assays and various oxidation models in vitro. Incubating the cells in the presence of this extract ameliorated t-butyl hydroperoxide-induced cytotoxicity from 27% to 57% in a macrophage model. The results may indicate the potential role of S. scilloides for its anti-inflammatory and antioxidative effects.


Nishida Y.,Tokai University | Nishida Y.,Aso Pharmaceutical Co. | Wada K.,Tokai University | Toyohisa D.,Tokai University | And 3 more authors.
Natural Product Research | Year: 2013

Scilla scilloides Druce has been used as a folk medicine to treat dermal inflammation; however, the medicinal property of this plant remains to be entirely clarified. The ethyl acetate extract prepared from bulbs of S. scilloides exhibited antioxidative activities on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydrogen peroxide (H2O2) and nitric oxide (NO) scavenging assays. Nine homoisoflavones (1-9) yielded from this extract were further examined for their antioxidative activities. Among these chemicals tested, five homoisoflavones (1-3, 5 and 7), six homoisoflavones (1-3 and 5-7) and two homoisoflavones (4 and 5) resulted in showing higher activities than the others in DPPH radical, H2O2 and NO scavenging assays, respectively. Calculated EC50 values indicate 3 as the strongest in the DPPH radical scavenging analysis. These results may indicate a potential role of S. scilloides for its medicinal use and homoisoflavones as the antioxidants responsible. © 2013 © 2013 Taylor & Francis.

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