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Cuneo, Italy

Vincenti M.,Centro Regionale Antidoping A Bertinaria | Vincenti M.,University of Turin | Leporati M.,University of Turin | Capra P.,C O Centro Regionale Antidoping A Bertinaria | And 4 more authors.
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2012

Prednisolone is a synthetic glucocorticoid widely employed in bovine clinical practice that may also be used illegally as a growth promoter. Recent in vitro and in vivo studies lend support to the hypothesis that prednisolone could be synthesised from cortisol in untreated cattle subjected to stressful events. To verify such a hypothesis, a field survey was conducted on urine samples collected from 131 guaranteed untreated cows and analysed for the presence of prednisolone and prednisone - in some instances also for cortisol and cortisone - with a validated LC/MS-MS method. None of the examined samples exhibited either prednisolone levels higher than the CCα limit (around 0.70 μg l-1) or prednisone, being therefore officially compliant for both analytes. Trace amounts of prednisolone, approximately estimated in the range 0.1-0.3 μg l-1 were found in only seven samples from cows also showing urinary cortisol and cortisone levels higher than those detected in negative specimens, as the result of a probable stress condition. © 2012 Copyright Taylor and Francis Group, LLC. Source

Uslenghi F.,University of Turin | Divari S.,University of Turin | Cannizzo F.T.,University of Turin | de Maria R.,University of Turin | And 7 more authors.
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2013

It has been previously demonstrated that the progesterone receptor gene is up-regulated in the sex accessory glands of pre-pubertal and adult male bovines after 17β-oestradiol treatment. In the present study, a qualitative screening method was optimised to detect 17β-oestradiol treatment using absolute quantification by qPCR of the progesterone receptor gene to determine the amount of gene expression in bulbo-urethral glands. An external standard curve was generated and developed with TaqMan® technology. Based on two in vivo experiments, the decision limit CCα, sensitivity and specificity of this screening method were established. Trial 1 consisted of 32 Friesian veal calves divided into two groups: group A (n = 12), consisting of animals treated with four doses of 17β-oestradiol (5 mg week-1 per animal); and group B (n = 20), consisting of control animals. Trial 2 was performed on 26 Charolaise beef cattle that either received five doses of 17β-oestradiol (group C; 20 mg week-1 per animal; n = 6) or remained untreated (group D; n = 20). Further, progesterone receptor gene expression was evaluated in beef and veal calves for human consumption. A specific CCα on 20 Piedmontese control beef cattle was calculated to include these animals in a field investigation. Five out of 190 beef cattle and 26 out of 177 calves tested expressed the progesterone receptor gene above their respective CCα and they were classified as being suspected of 17β-oestradiol treatment. Additionally, 58% of veal calves that tested suspect via qPCR exhibited histological lesions of the bulbo-urethral gland tissue, which are typical of oestrogen administration and are consistent with hyperplasia and metaplasia of the glandular epithelium. © 2013 Copyright Taylor and Francis Group, LLC. Source

Aim. Can the tension-free suturless technique, used in the surgical treatment of inguinal hernia, to be the gold standard for treatment of inguinal hernia? Methods. The tension-free suturless technique is often criticized as a fundamental principle: do not have suture. The criticism stems from concern that the mesh can migrate and cause damage to important anatomical structures. We conducted a study on the mobility of prosthesis on 33 patients, by using titanium clips that we have fixed on the meshes corner, X-rays over time, done at last, a follow-up often years. Results. The study shows that the prosthesis moves together with the anatomical space in which there is the forces present in the inguinal canal: gravity, intra-abdominal pressure, reactive force ascending gait. Across thirty-three patients have relapsed in the first six months and two recurrences in ten years, in the reconstitution of the neo-orifice, through which passes the cord.the remaining patients the mesh were relocated upward and medially (as identified by the clips of the increase of 10-15%). Conclusion. Our study shows that the mesh migrates upwards and medially. Migration is more or less, depending on the patient's age and quality of its tissue. Fix the prosthesis is good practice to secure at the flag on the inguinal ligament leads to two advantages: not to frustrate the principle tension-free, since the fixed prosthesis on one side does not create moments of tension, and prevent the prosthesis returns to the opening road to relapse. Source

Magro G.,ASO S. Croce e Carle | Barbero L.,ASL CN1 | Risso R.,ASL CN1 | Dardanelli L.,ASL CN1
Giornale Italiano di Diabetologia e Metabolismo | Year: 2010

Certain basic principles are necessary in order to implement disease management: the will of the State Institutions (Regional Council and Directorate-General of the Local Health Unit) in this regard, the cooperation of all the professional associations, the presence of tools to help both work organization and clinical management, and the starting up of a monitoring scheme through the measurement of process and result indicators. The history of the disease management of type 2 diabetes in Cuneo developed from the consideration of certain diabetologists regarding the lack of credibility of official epidemiological data (Regional Diabetics Register). A tool for clinical management was then set up (PDT, Percorso Diagnostico Terapeutico - Therapeutic Diagnostic Path) and over 5 to 6 years a more precise and complete organization of the situation was achieved, made possible also by the launch of the Regional scheme for the prevention of the complications of type 2 diabetes in the larger framework of the National prevention scheme. Source

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