Cuneo, Italy
Cuneo, Italy
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Biasibetti E.,University of Turin | Paciello O.,University Naples | Botta M.,ASL CN1 | Amedeo S.,University of Turin | Capucchio M.T.,University of Turin
Large Animal Review | Year: 2012

This study describes macroscopical and histological features of three cases of lipomatous myopathy (substitution of muscle tissue with adipose tissue) in Piedmontese cows. Several muscle samples, frozen in isopentane cooled using liquid nitrogen, were submitted to histological investigations. Slide sections (10 μm thick) were obtained using a cryostat and stained by the following histological and histochemical techniques: haematoxylin and eosin (H&E); modified Gomori trichrome; periodic acid-Schiff (PAS); red Oil stains, Sudan black, Sudan III, Cytochrome C oxidase (COX); succinate dehydrogenase (SDH), and reduced nicotinamide adenine dinucleotide-tetrazolium reductase (NADH-TR). Immunohistochemical stainings were also made employing specific antibodies to characterize the inflammatory cells. Gross pathology showed a different grade of infiltration of adipose tissue involving multiple or single muscles. Morphological staining revealed an infiltration of adipose tissue varying in distribution and severity, changes in muscle fiber size, and increased numbers of fibers with centrally located nuclei suggesting muscle degeneration- regeneration. Necrosis and non suppurative inflammatory cells were also seen. Furthermore, proliferation of connective tissue and non-specific myopathic changes, were present too. Histochemical stains confirmed this fat deposition but didn'st reveal metabolic changes in affected muscles. Sporadic focal immunopositivity for CD79, CD8, CD4 and MCHI were detected. Common usage of the world myopathy is restrict to describe a disorder of muscle generally characterized by different grade of degeneration of muscle fibers and high serum level of muscle enzymes. Myopathy are divided into several entities, such as diseases of the lower motor neuron, dystrophies, congenital myopathies, nutritional myopathies, metabolic and endocrine myopathies, inflammatory myopathies, and a miscellaneus group. Similarly to humans the classification of myopathies in animals is subject to modification based on findings of on-going genetic and pathogenetic studies as well as on changes in disease definitions. Vascular factors and neurogenic causes seem not involved in the pathogenesis of these cases of lipomatous myopathy. In next step the authors will delve into the role of genetic factors.

Uslenghi F.,University of Turin | Divari S.,University of Turin | Cannizzo F.T.,University of Turin | de Maria R.,University of Turin | And 7 more authors.
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2013

It has been previously demonstrated that the progesterone receptor gene is up-regulated in the sex accessory glands of pre-pubertal and adult male bovines after 17β-oestradiol treatment. In the present study, a qualitative screening method was optimised to detect 17β-oestradiol treatment using absolute quantification by qPCR of the progesterone receptor gene to determine the amount of gene expression in bulbo-urethral glands. An external standard curve was generated and developed with TaqMan® technology. Based on two in vivo experiments, the decision limit CCα, sensitivity and specificity of this screening method were established. Trial 1 consisted of 32 Friesian veal calves divided into two groups: group A (n = 12), consisting of animals treated with four doses of 17β-oestradiol (5 mg week-1 per animal); and group B (n = 20), consisting of control animals. Trial 2 was performed on 26 Charolaise beef cattle that either received five doses of 17β-oestradiol (group C; 20 mg week-1 per animal; n = 6) or remained untreated (group D; n = 20). Further, progesterone receptor gene expression was evaluated in beef and veal calves for human consumption. A specific CCα on 20 Piedmontese control beef cattle was calculated to include these animals in a field investigation. Five out of 190 beef cattle and 26 out of 177 calves tested expressed the progesterone receptor gene above their respective CCα and they were classified as being suspected of 17β-oestradiol treatment. Additionally, 58% of veal calves that tested suspect via qPCR exhibited histological lesions of the bulbo-urethral gland tissue, which are typical of oestrogen administration and are consistent with hyperplasia and metaplasia of the glandular epithelium. © 2013 Copyright Taylor and Francis Group, LLC.

Vincenti M.,Centro Regionale Antidoping A Bertinaria | Vincenti M.,University of Turin | Leporati M.,University of Turin | Capra P.,Instituto Zooprofilattico Sperimentale del Piemonte | And 4 more authors.
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2012

Prednisolone is a synthetic glucocorticoid widely employed in bovine clinical practice that may also be used illegally as a growth promoter. Recent in vitro and in vivo studies lend support to the hypothesis that prednisolone could be synthesised from cortisol in untreated cattle subjected to stressful events. To verify such a hypothesis, a field survey was conducted on urine samples collected from 131 guaranteed untreated cows and analysed for the presence of prednisolone and prednisone - in some instances also for cortisol and cortisone - with a validated LC/MS-MS method. None of the examined samples exhibited either prednisolone levels higher than the CCα limit (around 0.70 μg l-1) or prednisone, being therefore officially compliant for both analytes. Trace amounts of prednisolone, approximately estimated in the range 0.1-0.3 μg l-1 were found in only seven samples from cows also showing urinary cortisol and cortisone levels higher than those detected in negative specimens, as the result of a probable stress condition. © 2012 Copyright Taylor and Francis Group, LLC.

Aim. Can the tension-free suturless technique, used in the surgical treatment of inguinal hernia, to be the gold standard for treatment of inguinal hernia? Methods. The tension-free suturless technique is often criticized as a fundamental principle: do not have suture. The criticism stems from concern that the mesh can migrate and cause damage to important anatomical structures. We conducted a study on the mobility of prosthesis on 33 patients, by using titanium clips that we have fixed on the meshes corner, X-rays over time, done at last, a follow-up often years. Results. The study shows that the prosthesis moves together with the anatomical space in which there is the forces present in the inguinal canal: gravity, intra-abdominal pressure, reactive force ascending gait. Across thirty-three patients have relapsed in the first six months and two recurrences in ten years, in the reconstitution of the neo-orifice, through which passes the cord.the remaining patients the mesh were relocated upward and medially (as identified by the clips of the increase of 10-15%). Conclusion. Our study shows that the mesh migrates upwards and medially. Migration is more or less, depending on the patient's age and quality of its tissue. Fix the prosthesis is good practice to secure at the flag on the inguinal ligament leads to two advantages: not to frustrate the principle tension-free, since the fixed prosthesis on one side does not create moments of tension, and prevent the prosthesis returns to the opening road to relapse.

Buffoni L.,University of Turin | Vavala T.,ASL CN1 | Novello S.,University of Turin
Current Treatment Options in Oncology | Year: 2016

Twenty years ago, an individual patient data meta-analysis of eight cisplatin-based adjuvant chemotherapy (AC) studies in completely resected early stage non-small cell lung cancer (NSCLC) demonstrated a 13 % reduction of the risk of death favoring chemotherapy that was of borderline statistical significance (p = 0.08). This marginal benefit boosted a new generation of randomized trials to evaluate the role of modern platinum-based regimens in resectable stages of NSCLC and, although individual studies generated conflicting results, overall they contributed to confirm the role of AC which is now recommended for completely resected stage II and III NSCLC, mostly 4 cycles, while subset analyses suggested a benefit in patients with large IB tumors. Cisplatin-based therapy was the core regimen of those adjuvant clinical trials and even if a substitution with other platinum-derived was also suggested, mainly based on extrapolated data from studies in advanced disease, cisplatin was confirmed to be slightly superior to carboplatin and is still the drug of choice in the adjuvant setting. Currently, any attempt to improve efficacy of cisplatin-based chemotherapy through antiangiogenic drugs association or pharmacogenomics approaches have failed, while results of additional studies are eagerly awaited. In the context of promising targeted therapies, even if several randomized trials in the advanced setting evaluated tyrosine kinase inhibitors (TKis) versus platinum-based chemotherapy and showed impressive results, clinical experience with TKIs in the adjuvant setting is still limited and most of the trials have not required patients to be molecularly tested for the drug-specific molecular predictive factor. At the present time, the role of targeted agents as adjuvant approaches remains largely not investigated. Finally, with the negative experience of the use of vaccines in this setting, the integration of immunotherapy (mainly immunocheckpoint inhibitors) in platinum-based schedules has just started to be evaluated, representing a potential future clinical option, but still far from clinical practice. © 2016, Springer Science+Business Media New York.

PubMed | University of Turin and ASL CN1
Type: Journal Article | Journal: Italian journal of food safety | Year: 2016

The aim of this study is to evaluate different aspects regarding culled cattle and to suggest operating procedures for their correct management. Information collected in Piedmont region allowed for an assessment of the number of cattle put down on the farm, a quantification of slaughters performed in urgency and emergency (SUS/SES) and a headcount of those which died during farming. The survey highlighted the limited use of euthanasia or putting down compared to the number of cases of SUS/SES which were approximately ten times higher. If cattle displays severe health problems, such as a multifactorial disease like downer cow, the farmer has to decide rapidly the treatment to avoid cattle distress. A checklist has been developed and a flow chart has been revised to assist farmers and vets to quicken the decision-taking process and to manage the cattle in a more efficient manner. During this study a number of different problems have been stressed out. Particularly, the shortcomings in the training of operators commissioned to manage the animals, the inadequacy of structures used for the sheltering and slaughter of bovines on the farm, and differences in the operating procedures for culled cattle across the territory. From the obtained results, we can conclude that it is necessary to adopt a transversal approach, so that the information regarding these animals (welfare, health status, drug treatments and destination) will be uniform and adequate during all the steps of production, to ensure animal welfare and food safety.

Magro G.,ASO S. Croce e Carle | Barbero L.,ASL CN1 | Risso R.,ASL CN1 | Dardanelli L.,ASL CN1
Giornale Italiano di Diabetologia e Metabolismo | Year: 2010

Certain basic principles are necessary in order to implement disease management: the will of the State Institutions (Regional Council and Directorate-General of the Local Health Unit) in this regard, the cooperation of all the professional associations, the presence of tools to help both work organization and clinical management, and the starting up of a monitoring scheme through the measurement of process and result indicators. The history of the disease management of type 2 diabetes in Cuneo developed from the consideration of certain diabetologists regarding the lack of credibility of official epidemiological data (Regional Diabetics Register). A tool for clinical management was then set up (PDT, Percorso Diagnostico Terapeutico - Therapeutic Diagnostic Path) and over 5 to 6 years a more precise and complete organization of the situation was achieved, made possible also by the launch of the Regional scheme for the prevention of the complications of type 2 diabetes in the larger framework of the National prevention scheme.

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