Asia Seed Co.

Ich'ŏn, South Korea

Asia Seed Co.

Ich'ŏn, South Korea
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Kim I.,Food Republic | Ryu K.O.,Asia Seed Co.
Journal of Food Biochemistry | Year: 2016

This study was designed to investigate the potential reproductive health benefits of Raphanus sativus L. (radish) sprout ethanolic extract (RSE) in a bisphenol A (BPA)-exposed rat model of testicular disorders and to identify the bioactive components of RSE. RSE and its butanolic layer (RSE-BuOH) exhibited the strongest total antioxidant capacity, as determined using the oxygen radical absorbance capacity method. The RSE-BuOH was fractionated using chromatography with active guided fractionation, and 10 major compounds were identified in gas chromatography-mass spectrometry analysis. Among them, 3-hexyl-1-cyclopentene and 2-cyclopentene-1-tridecanoic acid were confirmed to be the major components. In addition, administration of RSE significantly restored body weight, testes weight and sperm cell rate in BPA-exposed rats. In particular, administration of RSE-BuOH to BPA-exposed rats significantly increased sperm cell rate and serum testosterone level. These results suggest that RSE may attenuate reproductive dysfunction caused by BPA-induced oxidative stress in male rats. © 2016 Wiley Periodicals, Inc.

Subburaj S.,Chungnam National University | Kim A.Y.,Sejong University | Lee S.,Sejong University | Kim K.-N.,Sejong University | And 3 more authors.
Plant Biotechnology Reports | Year: 2016

MicroRNAs (miRNAs) are a class of small non-coding, single-stranded RNA sequences that regulate gene expression at the post-transcriptional level and also reported to function in stress responses, but their role has not been studied in Camelina (Camelina sativa L.), an emerging oil crop. In this study, we predicted conserved as well as putative novel miRNAs from a Camelina drought stress cDNA library using comprehensive genomic approaches. Based on the sequence homology, we predicted 145 miRNAs, of which 61 were conserved, and 84 putative novel miRNAs were found to belong to 26 and 72 different miRNA families, respectively. In silico expression analysis indicated that 20 miRNAs were really expressed in Camelina genome, and several of them have tissue-specific expression character. We found that the 60 putative novel miRNA families target 117 genes. Most of the miRNA targets were predicted to genes including that regulate stress response, transcription factors, and fatty acid and lipid metabolism-related genes. Expression patterns of 6 randomly selected miRNAs under drought stress were validated by real-time quantitative polymerase chain reaction analysis. Coordinated expression changes between 6 randomly selected miRNAs and their target genes, suggested that the predicted miRNAs could be drought-responsive and that they would likely be directly involved in stress regulatory networks of Camelina. These results indicate that, in C. sativa, under drought stress, a large number of new miRNAs could be discovered, and the predicted stress-responsive miRNAs and their target transcripts will serve as valuable resources for future studies. © 2016, Korean Society for Plant Biotechnology and Springer Japan.

Kim G.-J.,Asia Seed Co. | Gi G.-Y.,Horticultural Research Division | Lee J.-H.,Chonnam National University | Song Y.-H.,Chonnam National University | And 3 more authors.
Horticulture Environment and Biotechnology | Year: 2011

Cultivars in the species Rosa hybrida L. can be classified into five septet groups according to their karyotypes. The objective of this study was to confirm the measurement of the septets using random amplified polymorphic DNA (RAPD) in various rose cultivars. Three species were comprised of four accessions, and 55 cultivars were subdivided into 37 standard and 18 miniature cultivars. The characteristics of the individual septets in three species were as follows: Rosa multiflora (WD1 and WD2) and R. indica (WD4) belonged to septet A and AA, respectively, and R. rugosa (WD3) belonged to septet C. Moreover, 55 rose cultivars were used in order to assess the contribution of the septets. Nineteen RAPD primers were used for an analysis. As a result of the dendrogram obtained from a cluster analysis, three different rose species were clearly allocated in accordance with their septets. Two accessions of R. multiflora (WD1 and WD2) were grouped together. In this study, the septet concept applied to identify the genetic variations of rose cultivars in more detail. Consequently, RAPD was a reasonable tool for the analysis of genetic backgrounds in rose cultivars. © Korean Society for Horticultural Science and Springer 2011.

Kim H.,Chonnam National University | Go Y.S.,Chonnam National University | Kim A.Y.,Sejong University | Lee S.,Sejong University | And 4 more authors.
Journal of Plant Biotechnology | Year: 2014

Camelina sativa that belongs to Brassicaceae family is an emerging oilseed crop. Camelina seeds contain approximately 40% storage oils per seed dry weight, which are useful for human and animal diets and industrial applications. Microsomal delta-12 fatty acid desaturase2 (FAD2) catalyzes the conversion of oleic acid to linoleic acid. The polymorphisms of FAD2 genes are correlated with the levels of oleic acids in seed oils. Microsomal delta-12 fatty acid desaturase2 (FAD2) catalyzes the conversion of oleic acid to linoleic acid. The polymorphisms of FAD2 genes are correlated with the levels of oleic acids in seed oils. In this study, three CsFAD2 genes (CsFAD2-1, CsFAD2-2 and CsFAD2-3.1) were isolated from developing seeds of Camelina sativa (L.) cv. CAME. The nucleotide and deduced amino acid sequences of three CsFAD2 genes were compared with those from dicotyledon and monocotyledon plants including Camelina cultivars Sunesone and SRS933. Three histidine motifs (HECGH, HRRHH, and HVAHH) required for FAD activity and a hydrophobic valine or isoleucine residue, which is a SNP (single nucleotide polymorphism) marker related with enzyme activity are well conserved in three CsFAD2s. The expr essi ons of CsFAD2-1 and CsFAD2-3.1 were ubiquitously detected in various Camelina organs, whereas the CsFAD2-2 transcripts were predominantly detected in flowers and developing seeds. The contents of oleic acids decreased, whereas the amounts of linoleic acid increased in dry seeds of transgenic fad2-2 lines expressing each CsFAD2 gene compared with fad2-2 mutant, indicating that three CsFAD2 genes are functionally active. The isolated CsFAD2 genes might be applicable in metabolic engineering of storage oils with high oleic acids in oilseed crops. © Korean Society for Plant Biotechnology.

Song J.-H.,Asia Seed Co. | Kim G.-J.,Asia Seed Co. | Kim K.-C.,Asia Seed Co. | Han T.-H.,Chonnam National University
Korean Journal of Horticultural Science and Technology | Year: 2014

A new resistant cabbage variety 'CT-171' to Fusarium wilt was bred by crossing A337MSBC5 with resistance to the disease and 397-G6 with good density and color. 'CT-171' was selected after combining ability, seed gathering and regional adaptability test in 2008. For breeding of resistant varieties, we investigated the development of Fusarium wilt on cabbage seedlings inoculated with Fusariumoxysporum f. sp. conglutinans by root dipping inoculation method. As a result, 'CT-171' showed higher resistance to Fusarium wilt than 'Asiaball' used as control. The maturity of new variety was 58 days and was faster than control and well suited for autumn cultivation because of cold resistance. The anthocyanin pigment of plant was not revealed. The weight, height and width of head were 1.5 kg, 14 cm and 15 cm, respectively and the core size was 5.7 cm and stable in various cultivation environments. 'CT-171' which showed good agricultural character and resistance to Fusarium wilt filed for variety protect right in Korea S eed & Variety S ervice on February 2013. The new v ariety will be appropriate for export and domestic consumption. © 2014 Korean Society for Horticultural Science.

Park H.J.,Korea Research Institute of Bioscience and Biotechnology | Jung W.Y.,Korea Research Institute of Bioscience and Biotechnology | Jung W.Y.,Gyeongnam National University of Science and Technology | Lee S.S.,Korea Research Institute of Bioscience and Biotechnology | And 6 more authors.
International Journal of Molecular Sciences | Year: 2013

Cabbage is a relatively robust vegetable at low temperatures. However, at high temperatures, cabbage has disadvantages, such as reduced disease tolerance and lower yields. Thus, selection of heat-tolerant cabbage is an important goal in cabbage breeding. Easier or faster selection of superior varieties of cabbage, which are tolerant to heat and disease and have improved taste and quality, can be achieved with molecular and biological methods. We compared heat-responsive gene expression between a heat-tolerant cabbage line (HTCL), -HO{norm of matrix}, and a heat-sensitive cabbage line (HSCL), -JK{norm of matrix}, by Genechip assay. Expression levels of specific heat stress-related genes were increased in response to high-temperature stress, according to Genechip assays. We performed quantitative RT-PCR (qRT-PCR) to compare expression levels of these heat stress-related genes in four HTCLs and four HSCLs. Transcript levels for heat shock protein BoHsp70 and transcription factor BoGRAS (SCL13) were more strongly expressed only in all HTCLs compared to all HSCLs, showing much lower level expressions at the young plant stage under heat stress (HS). Thus, we suggest that expression levels of these genes may be early selection markers for HTCLs in cabbage breeding. In addition, several genes that are involved in the secondary metabolite pathway were differentially regulated in HTCL and HSCL exposed to heat stress. © 2013 by the authors; licensee MDPI, Basel, Switzerland.

Thamil Arasan S.K.,Sunchon National University | Park J.-I.,Sunchon National University | Ahmed N.U.,Sunchon National University | Jung H.-J.,Sunchon National University | And 6 more authors.
Indian Journal of Experimental Biology | Year: 2013

Chinese cabbage (Brassica rapa) is widely recognized for its economic importance and contribution to human nutrition but abiotic and biotic stresses are main obstacle for its quality, nutritional status and production. In this study, 3,429 Express Sequence Tag (EST) sequences were generated from B. rapa cv. Osome cDNA library and the unique transcripts were classified functionally using a gene ontology (GO) hierarchy, Kyoto encyclopedia of genes and genomes (KEGG). KEGG orthology and the structural domain data were obtained from the biological database for stress related genes (SRG). EST datasets provided a wide outlook of functional characterization of B. rapa cv. Osome. In silico analysis revealed % 83 of ESTs to be well annotated towards reeds one dimensional concept. Clustering of ESTs returned 333 contigs and 2,446 singlets, giving a total of 3,284 putative unigene sequences. This dataset contained 1,017 EST sequences functionally annotated to stress responses and from which expression of randomly selected SRGs were analyzed against cold, salt, drought, ABA, water and PEG stresses. Most of the SRGs showed differentially expression against these stresses. Thus, the EST dataset is very important for discovering the potential genes related to stress resistance in chinese cabbage, and can be of useful resources for genetic engineering of Brassica sp.

Jiang N.,Chungnam National University | Chung S.-O.,Chungnam National University | Lee J.,Chungnam National University | Ryu D.,Chungnam National University | And 7 more authors.
Horticulture Environment and Biotechnology | Year: 2013

A newly developed red Chinese cabbage (Brassica rapa ssp. campestris; CC) was analyzed to determine the changes in functional compounds, especially in phenolic compounds. In the red CC, cyanidin was the major anthocyanidin, with 4. 11 mg·g-1 dry weight (dw), while pelargonidin and peonidin were minor components. The control CC, however, contained no anthocyanidins. In both CCs, four hydroxycinnamic acids were detected, and sinapic acid was the principal compound found. Hydroxycinnamic acids in the red CC were significantly more abundant than were those in the control, by a factor of 1. 4-13. 3. Quercetin was the principal flavonol in the red CC, with 2. 7 mg·g-1 dw, whereas it was the flavonol with the lowest concentration in the control CC (0. 5 mg·g-1 dw). Kaempferol and isorhamnetin concentrations were also increased in the red CC by 1. 41- and 3. 10-times, respectively. The results imply that, due to the common synthetic pathways of the phenolic compounds, the creation of a red phenotype from the presence of anthocyanidin increased the levels of flavonols and phenolic acids. Pectin, reducing sugar, and cellulose levels in the red CC indicate that the red CC could be preferred by consumers because of its better taste. Consequently, the new red CC, by accumulating significant amounts of the bioactive anthocyanidin, has significant value for human nutrition. © 2013 Korean Society for Horticultural Science.

PubMed | Sunchon National University and Asia Seed Ltd.
Type: Journal Article | Journal: Molecules (Basel, Switzerland) | Year: 2016

Glucosinolates have anti-carcinogenic properties. In the recent decades, the genetics of glucosinolate biosynthesis has been widely studied, however, the expression of specific genes involved in glucosinolate biosynthesis under exogenous phytohormone treatment has not been explored at the subspecies level in

Bae K.H.,National Institute of Biological Resources | Lee M.H.,Asiaseed Co. | Choi Y.E.,Kangwon National University | Yoon E.S.,Kongju National University
Journal of Plant Biotechnology | Year: 2014

Lychnis wilfordii (Regel) Maxim is a rare and valued ornamental plant. Germination rate reached 46.6% when seeds were treated with 100 mg·l -1 GA (Gibberellic acid)3. The highest callus induction was observed in the leaf explants of the seedlings on MS medium containing specific concentrations of 0.5 mg·l-1 BA (N 6-benzyladenine) and 3.0 mg·l-1 NAA (a-naphthalene acetic acid). The adventitious shoot was formed in 97.3% of calli on 1/2 WPM (Woody Plant Medium) medium. Shoot elongation of in vitro propagated plantlets was no difference among various medium. The plantlets grew well after transferring to the pot. This in vitro propagation protocol should be useful for conservation of this endangered plant. © 2014 Korean Society for Plant Biotechnology.

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