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Thamil Arasan S.K.,Sunchon National University | Park J.-I.,Sunchon National University | Ahmed N.U.,Sunchon National University | Jung H.-J.,Sunchon National University | And 6 more authors.
Indian Journal of Experimental Biology | Year: 2013

Chinese cabbage (Brassica rapa) is widely recognized for its economic importance and contribution to human nutrition but abiotic and biotic stresses are main obstacle for its quality, nutritional status and production. In this study, 3,429 Express Sequence Tag (EST) sequences were generated from B. rapa cv. Osome cDNA library and the unique transcripts were classified functionally using a gene ontology (GO) hierarchy, Kyoto encyclopedia of genes and genomes (KEGG). KEGG orthology and the structural domain data were obtained from the biological database for stress related genes (SRG). EST datasets provided a wide outlook of functional characterization of B. rapa cv. Osome. In silico analysis revealed % 83 of ESTs to be well annotated towards reeds one dimensional concept. Clustering of ESTs returned 333 contigs and 2,446 singlets, giving a total of 3,284 putative unigene sequences. This dataset contained 1,017 EST sequences functionally annotated to stress responses and from which expression of randomly selected SRGs were analyzed against cold, salt, drought, ABA, water and PEG stresses. Most of the SRGs showed differentially expression against these stresses. Thus, the EST dataset is very important for discovering the potential genes related to stress resistance in chinese cabbage, and can be of useful resources for genetic engineering of Brassica sp.

Kim G.-J.,Asia Seed Co. | Gi G.-Y.,Horticultural Research Division | Lee J.-H.,Chonnam National University | Song Y.-H.,Chonnam National University | And 3 more authors.
Horticulture Environment and Biotechnology | Year: 2011

Cultivars in the species Rosa hybrida L. can be classified into five septet groups according to their karyotypes. The objective of this study was to confirm the measurement of the septets using random amplified polymorphic DNA (RAPD) in various rose cultivars. Three species were comprised of four accessions, and 55 cultivars were subdivided into 37 standard and 18 miniature cultivars. The characteristics of the individual septets in three species were as follows: Rosa multiflora (WD1 and WD2) and R. indica (WD4) belonged to septet A and AA, respectively, and R. rugosa (WD3) belonged to septet C. Moreover, 55 rose cultivars were used in order to assess the contribution of the septets. Nineteen RAPD primers were used for an analysis. As a result of the dendrogram obtained from a cluster analysis, three different rose species were clearly allocated in accordance with their septets. Two accessions of R. multiflora (WD1 and WD2) were grouped together. In this study, the septet concept applied to identify the genetic variations of rose cultivars in more detail. Consequently, RAPD was a reasonable tool for the analysis of genetic backgrounds in rose cultivars. © Korean Society for Horticultural Science and Springer 2011.

Park H.J.,Korea Research Institute of Bioscience and Biotechnology | Jung W.Y.,Korea Research Institute of Bioscience and Biotechnology | Jung W.Y.,Gyeongnam National University of Science and Technology | Lee S.S.,Korea Research Institute of Bioscience and Biotechnology | And 6 more authors.
International Journal of Molecular Sciences | Year: 2013

Cabbage is a relatively robust vegetable at low temperatures. However, at high temperatures, cabbage has disadvantages, such as reduced disease tolerance and lower yields. Thus, selection of heat-tolerant cabbage is an important goal in cabbage breeding. Easier or faster selection of superior varieties of cabbage, which are tolerant to heat and disease and have improved taste and quality, can be achieved with molecular and biological methods. We compared heat-responsive gene expression between a heat-tolerant cabbage line (HTCL), -HO{norm of matrix}, and a heat-sensitive cabbage line (HSCL), -JK{norm of matrix}, by Genechip assay. Expression levels of specific heat stress-related genes were increased in response to high-temperature stress, according to Genechip assays. We performed quantitative RT-PCR (qRT-PCR) to compare expression levels of these heat stress-related genes in four HTCLs and four HSCLs. Transcript levels for heat shock protein BoHsp70 and transcription factor BoGRAS (SCL13) were more strongly expressed only in all HTCLs compared to all HSCLs, showing much lower level expressions at the young plant stage under heat stress (HS). Thus, we suggest that expression levels of these genes may be early selection markers for HTCLs in cabbage breeding. In addition, several genes that are involved in the secondary metabolite pathway were differentially regulated in HTCL and HSCL exposed to heat stress. © 2013 by the authors; licensee MDPI, Basel, Switzerland.

Bae K.H.,National Institute of Biological Resources | Lee M.H.,Asia Seed Co. | Choi Y.E.,Kangwon National University | Yoon E.S.,Kongju National University
Journal of Plant Biotechnology | Year: 2014

Lychnis wilfordii (Regel) Maxim is a rare and valued ornamental plant. Germination rate reached 46.6% when seeds were treated with 100 mg·l -1 GA (Gibberellic acid)3. The highest callus induction was observed in the leaf explants of the seedlings on MS medium containing specific concentrations of 0.5 mg·l-1 BA (N 6-benzyladenine) and 3.0 mg·l-1 NAA (a-naphthalene acetic acid). The adventitious shoot was formed in 97.3% of calli on 1/2 WPM (Woody Plant Medium) medium. Shoot elongation of in vitro propagated plantlets was no difference among various medium. The plantlets grew well after transferring to the pot. This in vitro propagation protocol should be useful for conservation of this endangered plant. © 2014 Korean Society for Plant Biotechnology.

Kim I.,Food Republic | Ryu K.O.,Asia Seed Co.
Journal of Food Biochemistry | Year: 2016

This study was designed to investigate the potential reproductive health benefits of Raphanus sativus L. (radish) sprout ethanolic extract (RSE) in a bisphenol A (BPA)-exposed rat model of testicular disorders and to identify the bioactive components of RSE. RSE and its butanolic layer (RSE-BuOH) exhibited the strongest total antioxidant capacity, as determined using the oxygen radical absorbance capacity method. The RSE-BuOH was fractionated using chromatography with active guided fractionation, and 10 major compounds were identified in gas chromatography-mass spectrometry analysis. Among them, 3-hexyl-1-cyclopentene and 2-cyclopentene-1-tridecanoic acid were confirmed to be the major components. In addition, administration of RSE significantly restored body weight, testes weight and sperm cell rate in BPA-exposed rats. In particular, administration of RSE-BuOH to BPA-exposed rats significantly increased sperm cell rate and serum testosterone level. These results suggest that RSE may attenuate reproductive dysfunction caused by BPA-induced oxidative stress in male rats. © 2016 Wiley Periodicals, Inc.

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