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Prasad K.S.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science | Selvaraj K.,Council of Scientific and Industrial Research
Biological Trace Element Research | Year: 2014

A bioreductive capacity of a plant, Terminalia arjuna leaf extract, was utilized for preparation of selenium nanoparticles. The leaf extract worked as good capping as well as stabilizing agent and facilitated the formation of stable colloidal nanoparticles. Resulting nanoparticles were characterized using UV-Vis spectrophotometer, transmission electron microscopy (TEM), energy dispersive X-ray analysis (EDAX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction analysis (XRD), respectively. The colloidal solution showed the absorption maximum at 390 nm while TEM and selected area electron diffraction (SAED) indicated the formation of polydispersed, crystalline selenium nanoparticles of size raging from 10 to 80 nm. FT-IR analysis suggested the involvement of O-H, N-H, C=O, and C-O functional group of the leaf extract in particle formation while EDAX analysis indicated the presence of selenium in synthesized nanoparticles. The effect of nanoparticles on human lymphocytes treated with arsenite, As(III), has been studied. Studies on cell viability using MTT assay and DNA damage using comet assay revealed that synthesized selenium nanoparticles showed protective effect against As(III)-induced cell death and DNA damage. Chronic ingestion of arsenic infested groundwater, and prevalence of arsenicosis is a serious public health issue. The synthesized benign nanoselenium can be a promising agent to check the chronic toxicity caused due to arsenic exposure. © 2014 Springer Science+Business Media New York. Source


Goswami D.,Gujarat University | Thakker J.N.,Gujarat University | Dhandhukia P.C.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science
Journal of Microbiological Methods | Year: 2015

A simple, quick and reliable method is proposed for the detection and quantitation of indole-3-acetate (IAA) and indole-3-butyrate (IBA), an auxin phytohormone produced by rhizobacteria from l-tryptophan (Trp) metabolism using high performance thin-layer chromatography (HPTLC). Microbial auxin biosynthesis routes involve Trp as a precursor where other than IAA and IBA, products such as indole-3-pyruvate (IPA), indole-3-acetamide (IAM), tryptamine, indole-3-acetonitrile (IAN), indole-3-lactic acid (ILA) and indole-3-acetaldehyde (IAAld) are also produced. In traditional spectrophotometric method, Salkowski reagent develops color by reacting with indolic compounds. The color development is non-specific contributed by several Trp derivatives produced by rhizobacteria rather than IAA only. To overcome this limitation, HPTLC based protocol is developed to precisely detect and quantify IAA and IBA in the range of 100 to 1000. ng per spot. This protocol is applicable to detect and quantify IAA and IBA from microbial samples ignoring other Trp derivatives. For microbial samples, the spectrophotometric method gives larger values as compared to HPTLC derived values which may be attributed by total indolic compounds reacting with Salkowski reagent rather than only IAA and/or IBA. © 2014. Source


Akhaja T.N.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science | Raval J.P.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science
Medicinal Chemistry Research | Year: 2013

A series of structurally new, 2-(5-substituted-2,3-dioxoindolin-1-yl)ethyl/ propyl 4-(3,4-dichlorophenyl)piperazine-1-carbodithioate derivatives 5a-j were designed and synthesized by conventional technique as well as ultrasound irradiation. All the new compounds were characterized by spectral and elemental analyses. Furthermore, they were evaluated for their in vitro antibacterial, antifungal, antitubercular, and antimalarial activities. The results indicated that some of the synthesized compounds posses promising antimicrobial activity against some gram-positive and gram-negative bacteria. Compounds 5b, 5d, and 5e displayed the highest inhibition (99 %) in the range of 3.10-6.25 μg/ml against Mycobacterium tuberculosis H 37 Rv, while compounds 5b-g displayed promising antimalarial activity in the range of 0.043-0.092 μg/mL against Plasmodium falciparum 3D7. Thus, these molecules can provide prospective leads in chemotherapy against tuberculosis and malaria. © 2013 Springer Science+Business Media New York. Source


Patel M.B.,Gujarat University | Modi N.R.,Gujarat University | Raval J.P.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science | Menon S.K.,Gujarat University
Organic and Biomolecular Chemistry | Year: 2012

In the present investigation, we describe some novel calixarene based heterocyclic compounds (5a-5i) in which 1,3,4-oxadiazole and 1,3,4-thiadiazole derivatives have been coupled with 5,11,17,23-tetra-tert-butyl-25,27- bis(chlorocarbonyl-methoxy)-26,28-dihydroxy calix[4]arene. All the newly synthesized calixarene based heterocyclic compounds have been characterized by elemental analysis and various spectroscopic methods like FTIR, 1H NMR, 13C NMR, and FAB-MS. All the final scaffolds have been subjected to antioxidant activity, in vitro antimicrobial screening against two gram (+ve) bacteria (S. aureus, S. pyogenes), two gram (-ve) bacteria (E. coli, P. aeruginosa) and two fungal strains (C. albicans, A. clavatus) and also have been screened for their antitubercular activity against Mycobacterium tuberculosis H 37Rv. © 2012 The Royal Society of Chemistry. Source


Akhaja T.N.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science | Raval J.P.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science
Chinese Chemical Letters | Year: 2012

A series of 5-substitued-3-(5-(4-(furan-2-yl)-6-methyl-2-oxo/thioxo-1,2,3, 4-tetrahydropyrimidin-5-yl)-1,3,4-thiadiazol-2-ylimino)indolin-2-one derivatives were synthesized, characterized and were screened for anti-bacterial, anti-fungal and anti-tubercular activity. © 2012 Jignesh Priyakant Raval. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved. Source

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