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Naik D.R.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS | Raval J.P.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS
Journal of Saudi Chemical Society | Year: 2012

The aim of this study was to develop and optimize a nanoparticulate carrier based on polymeric blends of cellulose acetate butyrate (CAB) and poly(vinyl pyrrolidone) (PVP) to enhance dissolution profile of an antiviral drug. These nanoparticles are studied by means of X-ray powder diffraction, FTIR and DSC while the morphology evaluated using SEM analysis. Drug-loaded nanoparticles were produced in spherical shape with sizes and encapsulation efficiency ranging from 322 to 434 nm and 50% to 70% respectively. The effects of different CAB/PVP ratios, concentration of drug and emulsifier on the nanoparticle size, drug encapsulation and in vitro release were studied in detail. In vitro release along with mechanism and kinetics were studied in different pHs indicating that the release of drug from nanoparticles was pH-responsive. All the nanoparticles displayed a slowed release pattern with the reduced burst release. The mechanism and kinetics of the drug delivery system was also systematically studied using various models such as zero order, first order, Higuchi model and Korsmeyer-Peppas. The results indicate that the new CAB/PVP nanoparticles have a promising potential to serve as an antiviral controlled delivery system. © 2012. Source


Prasad K.S.,M. S. University of Baroda | Amin T.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS | Katuva S.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS | Kumari M.,har University | Selvaraj K.,CSIR - National Chemical Laboratory
Arabian Journal of Chemistry | Year: 2014

This study reports a novel method for preparation of water soluble CdS nanoparticles using leaf extract of a plant, Asparagus racemosus. The extract of the leaf tissue which worked as a stabilizing and capping agent, assisted the formation of nanoparticles. Nanoparticles were characterized using a UV-vis spectrophotometer, Photoluminescence, TEM, EDAX, XRD and FT-IR. Transmission electron microscopy followed by selected area electron diffraction pattern analysis indicated the formation of spherical, polydispersed, crystalline, CdS of diameter ranging from 2 to 8. nm. X-ray diffraction studies showed the formation of 1. 1. 1, 2. 2. 0 and 3. 1. 1 planes of face-centered cubic (fcc) CdS. EDAX analysis confirmed the presence of Cd and S in nanosphere. The cytotoxicity test using MTT assay as well as DNA damage analysis using comet assay revealed that synthesized nano CdS quantum dots (QDs) caused less DNA damage and cell death of lymphocytes than pure CdS nanoparticles. © 2014 King Saud University. Source


Dholakia N.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS | Dhandhukia P.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS | Roy N.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS
Molecular Diversity | Year: 2015

The Apicomplexa parasite Plasmodium is a major cause of death in developing countries which are less equipped to bring new medicines to the market. Currently available drugs used for treatment of malaria are limited either by inadequate efficacy, toxicity and/or increased resistance. Availability of the genome sequence, microarray data and metabolic profile of Plasmodium parasite offers an opportunity for the identification of stage-specific genes important to the organism’s lifecycle. In this study, microarray data were analysed for differential expression and overlapped onto metabolic pathways to identify differentially regulated pathways essential for transition to successive erythrocytic stages. The results obtained indicate that S-adenosylmethionine decarboxylase/ornithine decarboxylase, a bifunctional enzyme required for polyamine synthesis, is important for the Plasmodium cell growth in the absence of exogenous polyamines. S-adenosylmethionine decarboxylase/ornithine decarboxylase is a valuable target for designing therapeutically useful inhibitors. One such inhibitor, α-difluoromethyl ornithine, is currently in use for the treatment of African sleeping sickness caused by Trypanosoma brucei. Structural studies of ornithine decarboxylase along with known inhibitors and their analogues were carried out to screen drug databases for more effective and less toxic compounds. © 2015, Springer International Publishing Switzerland. Source

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