Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS

Gujarat, India

Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS

Gujarat, India
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Nailwal M.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS | Chauhan J.B.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS
Journal of Clinical and Diagnostic Research | Year: 2017

Introduction: Azoospermia Factor (AZF) microdeletions in Yq chromosome is one of the most frequent genetic cause associated with failure of spermatogenesis in males with infertility. Aim: To figure out the Yq chromosome microdeletions frequency in infertile men from Gujarat region of India. Materials and Methods: In this study, 141 infertile men with azoospermia (n=41) and oligozoospermia (n=100) were examined along with 159 normozoospermic men. Eleven different markers spanning the azoospermia factor region of human Yq chromosome, amplified by sequence-tagged site polymerase chain reaction to detect the microdeletions. Sperm morphological analysis was done using papanicolau staining method. Results: Thirty four infertile men out of 141 presented Yq chromosome microdeletions. The frequency of AZF microdeletions was 31.71% in azoospermia and 21% in oligozoospermia patients. Only two oligozoospermia patients showed morphological defects. Conclusion: Due to the presence of high frequency of Yq chromosome microdeletions in Gujarati infertile men, it is imperative to implement the AZF microdeletion screening in such patients as it results in male spermatogenesis dysfunctioning. © 2007-2017, Journal of Clinical and Diagnostic Research, India.


Nailwal M.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS | Chauhan J.B.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS
Meta Gene | Year: 2017

Yq microdeletion is one of the well-known genetic causes of infertility in males which results in spermatogenetic impairment. Among the three regions (AZFa, AZFb and AZFc), deletion in Azoospermia Factor a (AZFa) sub-region of the human Y-chromosome considered to have most severe outcome and may cause spermatogenic failure where Sertoli-Cell Only (SCO) syndrome occur in clinically tested testis. Because of the deletion, men may not form or able to keep up the nascent germ cells cohort during adolescence and after that. The AZFa sub-region contains mainly three candidate genes, USP9Y (Ubiquitin specific peptidase 9, Y-linked), DBY (DEAD/H Box 3, Y-linked) and UTY (Ubiquitously Transcribed Tetratricopeptide Repeat Containing, Y-Linked) whose defined role are unknown. This review article makes understand about the deletion mechanism and all the three genes of AZFa sub-region related to infertility in men. © 2017 Elsevier B.V.


Banerjee D.,Ashok and Rita Patel Institute of Integrated Study and Research In Biotechnology And Allied science ARIBAS | Gohil T.,Ashok and Rita Patel Institute of Integrated Study and Research In Biotechnology And Allied science ARIBAS | Trivedi S.,Smt L P Patel Institute Of Medical Laboratory Technology
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2015

Objective: The aim of the present study was to isolate an antimicrobial peptide from heart tissue of Capra hircus (Goat) and to check its antibacterial activity against antibiotic resistant and antibiotic sensitive bacterial strains. Methods: Total protein was extracted from heart tissue of goat using extraction buffer, purified by cation exchange column chromatography and tested in vitro for antibacterial activity against MTCC bacterial strains as well as clinical isolates using the agar well diffusion method. MIC was also checked by microbroth dilution method. Mass spectrometry was performed for partially purified protein fraction. Results: The present work revealed that crude protein extracted from the heart tissue of goat was found to have antimicrobial activity. Partially purified protein fraction also showed the zone of inhibition and MIC value against all the bacteria tested. Mass spectrometry data showed the molecular weight of the peptide to be ~6.5 kD along with peptide to be present in the oligomeric form. Conclusion: The present study showed that the defens in is an antimicrobial peptide with broad range of effect which is present in dimer, trimer and oligomeric form. © 2015, IJPPS. All rights reserved.


Naik D.R.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS | Raval J.P.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS
Journal of Saudi Chemical Society | Year: 2012

The aim of this study was to develop and optimize a nanoparticulate carrier based on polymeric blends of cellulose acetate butyrate (CAB) and poly(vinyl pyrrolidone) (PVP) to enhance dissolution profile of an antiviral drug. These nanoparticles are studied by means of X-ray powder diffraction, FTIR and DSC while the morphology evaluated using SEM analysis. Drug-loaded nanoparticles were produced in spherical shape with sizes and encapsulation efficiency ranging from 322 to 434 nm and 50% to 70% respectively. The effects of different CAB/PVP ratios, concentration of drug and emulsifier on the nanoparticle size, drug encapsulation and in vitro release were studied in detail. In vitro release along with mechanism and kinetics were studied in different pHs indicating that the release of drug from nanoparticles was pH-responsive. All the nanoparticles displayed a slowed release pattern with the reduced burst release. The mechanism and kinetics of the drug delivery system was also systematically studied using various models such as zero order, first order, Higuchi model and Korsmeyer-Peppas. The results indicate that the new CAB/PVP nanoparticles have a promising potential to serve as an antiviral controlled delivery system. © 2012.


Dholakia N.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS | Dhandhukia P.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS | Roy N.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS
Molecular Diversity | Year: 2015

The Apicomplexa parasite Plasmodium is a major cause of death in developing countries which are less equipped to bring new medicines to the market. Currently available drugs used for treatment of malaria are limited either by inadequate efficacy, toxicity and/or increased resistance. Availability of the genome sequence, microarray data and metabolic profile of Plasmodium parasite offers an opportunity for the identification of stage-specific genes important to the organism’s lifecycle. In this study, microarray data were analysed for differential expression and overlapped onto metabolic pathways to identify differentially regulated pathways essential for transition to successive erythrocytic stages. The results obtained indicate that S-adenosylmethionine decarboxylase/ornithine decarboxylase, a bifunctional enzyme required for polyamine synthesis, is important for the Plasmodium cell growth in the absence of exogenous polyamines. S-adenosylmethionine decarboxylase/ornithine decarboxylase is a valuable target for designing therapeutically useful inhibitors. One such inhibitor, α-difluoromethyl ornithine, is currently in use for the treatment of African sleeping sickness caused by Trypanosoma brucei. Structural studies of ornithine decarboxylase along with known inhibitors and their analogues were carried out to screen drug databases for more effective and less toxic compounds. © 2015, Springer International Publishing Switzerland.


Desai M.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS | Chauhan J.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS
Meta Gene | Year: 2016

Methyl CpG binding protein 2 is an abundant chromatin associated protein helps in transcriptional regulation. The MECP2 gene mutations are found to be associated with many diseases including moderate to severe X-linked mental retardation, Rett syndrome, autism and neonatal encephalopathy. So many mutations till date are reported in MECP2 gene. Only dbSNP contain > 3000 SNPs in human MECP2 gene which all may not be deleterious. Therefore, it is advisable to sort out the SNPs which may alter the protein function, before experimentation on large population. To fulfill those criteria we analyzed nsSNPs from the dbSNP using various computational tools like SIFT, PANTHER, PolyPhen, SNAP2, SNPs&GO. Two SNPs, T170M and R318C predicted as the most deleterious SNPs using above tools. I-Mutant and MuSTAB showed decreased protein stability for both mutants. The structural analysis of the native and mutant protein was also performed; MUSTER was used to generate the 3D model of the proteins. The 3D structure analysis and energy minimization was done using Swiss-PdbViewer. In-Silico analysis suggested that T170M and R318C variants of MECP2gene can cause alteration in the protein structure and function. Screening of these variants may be useful in disease molecular diagnosis. © 2016 Elsevier B.V.


Prasad K.S.,M. S. University of Baroda | Amin T.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS | Katuva S.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS | Kumari M.,har University | Selvaraj K.,CSIR - National Chemical Laboratory
Arabian Journal of Chemistry | Year: 2014

This study reports a novel method for preparation of water soluble CdS nanoparticles using leaf extract of a plant, Asparagus racemosus. The extract of the leaf tissue which worked as a stabilizing and capping agent, assisted the formation of nanoparticles. Nanoparticles were characterized using a UV-vis spectrophotometer, Photoluminescence, TEM, EDAX, XRD and FT-IR. Transmission electron microscopy followed by selected area electron diffraction pattern analysis indicated the formation of spherical, polydispersed, crystalline, CdS of diameter ranging from 2 to 8. nm. X-ray diffraction studies showed the formation of 1. 1. 1, 2. 2. 0 and 3. 1. 1 planes of face-centered cubic (fcc) CdS. EDAX analysis confirmed the presence of Cd and S in nanosphere. The cytotoxicity test using MTT assay as well as DNA damage analysis using comet assay revealed that synthesized nano CdS quantum dots (QDs) caused less DNA damage and cell death of lymphocytes than pure CdS nanoparticles. © 2014 King Saud University.


PubMed | Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science ARIBAS
Type: Journal Article | Journal: Molecular diversity | Year: 2015

The Apicomplexa parasite Plasmodium is a major cause of death in developing countries which are less equipped to bring new medicines to the market. Currently available drugs used for treatment of malaria are limited either by inadequate efficacy, toxicity and/or increased resistance. Availability of the genome sequence, microarray data and metabolic profile of Plasmodium parasite offers an opportunity for the identification of stage-specific genes important to the organisms lifecycle. In this study, microarray data were analysed for differential expression and overlapped onto metabolic pathways to identify differentially regulated pathways essential for transition to successive erythrocytic stages. The results obtained indicate that S-adenosylmethionine decarboxylase/ornithine decarboxylase, a bifunctional enzyme required for polyamine synthesis, is important for the Plasmodium cell growth in the absence of exogenous polyamines. S-adenosylmethionine decarboxylase/ornithine decarboxylase is a valuable target for designing therapeutically useful inhibitors. One such inhibitor, [Formula: see text]-difluoromethyl ornithine, is currently in use for the treatment of African sleeping sickness caused by Trypanosoma brucei. Structural studies of ornithine decarboxylase along with known inhibitors and their analogues were carried out to screen drug databases for more effective and less toxic compounds.

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