Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science

Ānand, India

Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science

Ānand, India

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Pagedar A.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science | Singh J.,National Dairy Research Institute | Singh J.,PPD Group
International Dairy Journal | Year: 2012

The effect of initial physiological stages, i.e., sessile, vegetative, and spore (SP), of Bacillus cereus on its biofilm formation potential was investigated. Enhanced biofilm formation was observed in diluted tryptic soy broth in comparison with milk menstruum. Growth rate constants of the biofilm growth curve (BGC) indicated that SP was the most efficient cell stage to initiate and form a biofilm. Comparative evaluation of the BGC and suspension growth curve revealed that former's lag phase corresponded to latter's log phase (4-16h). Through being surface adhered and in lag phase, these cells might be more resistant to cleaning. Moreover, the greater SP percentage in spore initiated biofilm than spore initiated suspension presents an obvious food safety threat. These findings suggest a noteworthy effect of initial cell stages on biofilm formation potential of B.cereus and may be helpful in designing/scheduling more efficient interventions to minimize associated health hazards. © 2011 Elsevier Ltd.


Patel M.B.,Gujarat University | Modi N.R.,Gujarat University | Raval J.P.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science | Menon S.K.,Gujarat University
Organic and Biomolecular Chemistry | Year: 2012

In the present investigation, we describe some novel calixarene based heterocyclic compounds (5a-5i) in which 1,3,4-oxadiazole and 1,3,4-thiadiazole derivatives have been coupled with 5,11,17,23-tetra-tert-butyl-25,27- bis(chlorocarbonyl-methoxy)-26,28-dihydroxy calix[4]arene. All the newly synthesized calixarene based heterocyclic compounds have been characterized by elemental analysis and various spectroscopic methods like FTIR, 1H NMR, 13C NMR, and FAB-MS. All the final scaffolds have been subjected to antioxidant activity, in vitro antimicrobial screening against two gram (+ve) bacteria (S. aureus, S. pyogenes), two gram (-ve) bacteria (E. coli, P. aeruginosa) and two fungal strains (C. albicans, A. clavatus) and also have been screened for their antitubercular activity against Mycobacterium tuberculosis H 37Rv. © 2012 The Royal Society of Chemistry.


Akhaja T.N.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science | Raval J.P.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science
Medicinal Chemistry Research | Year: 2013

A series of structurally new, 2-(5-substituted-2,3-dioxoindolin-1-yl)ethyl/ propyl 4-(3,4-dichlorophenyl)piperazine-1-carbodithioate derivatives 5a-j were designed and synthesized by conventional technique as well as ultrasound irradiation. All the new compounds were characterized by spectral and elemental analyses. Furthermore, they were evaluated for their in vitro antibacterial, antifungal, antitubercular, and antimalarial activities. The results indicated that some of the synthesized compounds posses promising antimicrobial activity against some gram-positive and gram-negative bacteria. Compounds 5b, 5d, and 5e displayed the highest inhibition (99 %) in the range of 3.10-6.25 μg/ml against Mycobacterium tuberculosis H 37 Rv, while compounds 5b-g displayed promising antimalarial activity in the range of 0.043-0.092 μg/mL against Plasmodium falciparum 3D7. Thus, these molecules can provide prospective leads in chemotherapy against tuberculosis and malaria. © 2013 Springer Science+Business Media New York.


Akhaja T.N.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science | Raval J.P.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science
Chinese Chemical Letters | Year: 2012

A series of 5-substituted-3-[{5-(6-methyl-2-oxo/thioxo-4-phenyl-1,2,3,4- tetrahydropyrimidin-5-yl)-1,3,4-oxadiazol-2-yl}-imino]-1,3-dihydro-2H-indol-2- one were synthesized, characterized and screened for their anti-tubercular and antimalarial activity. © 2012 Jignesh Priyakant Raval.


Myangar K.N.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science | Raval J.P.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science
Medicinal Chemistry Research | Year: 2012

A series of 2-[(4'-oxo-3'-chloro-2'-phenylazetidin- 1'-ylamino)-methyl]-3- [N-isonicotinamide-yl]-quinazolin- 4-one hybrids were synthesized starting from anthranilic acid in basic media using chloroacetyl chloride and benzene as solvent. The structure of the synthesized compounds has been evaluated on the basis of their elemental (C, H, and N) and spectral analysis (IR, 1H NMR, and 13CNMR spectrometry). In vitro antimicrobial evaluation of the synthesized compounds revealed that they posses promising antimicrobial activity against some gram-positive and gram-negative bacteria. Furthermore, compounds 7d and 7f exhibited potent antitubercular activity. © Springer Science+Business Media, LLC 2011.


Akhaja T.N.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science | Raval J.P.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science
Chinese Chemical Letters | Year: 2012

A series of 5-substitued-3-(5-(4-(furan-2-yl)-6-methyl-2-oxo/thioxo-1,2,3, 4-tetrahydropyrimidin-5-yl)-1,3,4-thiadiazol-2-ylimino)indolin-2-one derivatives were synthesized, characterized and were screened for anti-bacterial, anti-fungal and anti-tubercular activity. © 2012 Jignesh Priyakant Raval. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.


Goswami D.,Gujarat University | Thakker J.N.,Gujarat University | Dhandhukia P.C.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science
Journal of Microbiological Methods | Year: 2015

A simple, quick and reliable method is proposed for the detection and quantitation of indole-3-acetate (IAA) and indole-3-butyrate (IBA), an auxin phytohormone produced by rhizobacteria from l-tryptophan (Trp) metabolism using high performance thin-layer chromatography (HPTLC). Microbial auxin biosynthesis routes involve Trp as a precursor where other than IAA and IBA, products such as indole-3-pyruvate (IPA), indole-3-acetamide (IAM), tryptamine, indole-3-acetonitrile (IAN), indole-3-lactic acid (ILA) and indole-3-acetaldehyde (IAAld) are also produced. In traditional spectrophotometric method, Salkowski reagent develops color by reacting with indolic compounds. The color development is non-specific contributed by several Trp derivatives produced by rhizobacteria rather than IAA only. To overcome this limitation, HPTLC based protocol is developed to precisely detect and quantify IAA and IBA in the range of 100 to 1000. ng per spot. This protocol is applicable to detect and quantify IAA and IBA from microbial samples ignoring other Trp derivatives. For microbial samples, the spectrophotometric method gives larger values as compared to HPTLC derived values which may be attributed by total indolic compounds reacting with Salkowski reagent rather than only IAA and/or IBA. © 2014.


Prasad K.S.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science | Selvaraj K.,Council of Scientific and Industrial Research
Biological Trace Element Research | Year: 2014

A bioreductive capacity of a plant, Terminalia arjuna leaf extract, was utilized for preparation of selenium nanoparticles. The leaf extract worked as good capping as well as stabilizing agent and facilitated the formation of stable colloidal nanoparticles. Resulting nanoparticles were characterized using UV-Vis spectrophotometer, transmission electron microscopy (TEM), energy dispersive X-ray analysis (EDAX), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction analysis (XRD), respectively. The colloidal solution showed the absorption maximum at 390 nm while TEM and selected area electron diffraction (SAED) indicated the formation of polydispersed, crystalline selenium nanoparticles of size raging from 10 to 80 nm. FT-IR analysis suggested the involvement of O-H, N-H, C=O, and C-O functional group of the leaf extract in particle formation while EDAX analysis indicated the presence of selenium in synthesized nanoparticles. The effect of nanoparticles on human lymphocytes treated with arsenite, As(III), has been studied. Studies on cell viability using MTT assay and DNA damage using comet assay revealed that synthesized selenium nanoparticles showed protective effect against As(III)-induced cell death and DNA damage. Chronic ingestion of arsenic infested groundwater, and prevalence of arsenicosis is a serious public health issue. The synthesized benign nanoselenium can be a promising agent to check the chronic toxicity caused due to arsenic exposure. © 2014 Springer Science+Business Media New York.


Akhaja T.N.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science | Raval J.P.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science
European Journal of Medicinal Chemistry | Year: 2011

1,3-dihydro-2H-indol-2-ones derivatives are reported to exhibit a wide variety of biodynamic activities such as antituberculer, anti HIV, fungicidal, antibacterial, anticonvulsant. These valid observations led us to synthesize some new indole-2-one derivative. Thus, herein we report synthesis of various 5-substituted-3-[{5-(6-methyl-2-oxo/thioxo-4-phenyl-1,2,3,4 tetrahydro pyrimidin-5-yl)-1,3,4-thiadiazol-2-yl}imino]-1,3-dihydro-2H-indol-2-one derivatives 4a-l using one pot multicomponent-Biginelli reaction via CaCl 2 catalyst. Structures and purity of these compounds were confirmed by elemental, IR, ( 1H & 13C) NMR and Mass spectral analysis. Newly synthesized compounds were also tested for their in vitro anti-tubercular activity against Mycobacterium tuberculosis H 37Rv, in vitro antibacterial activity against selected human pathogens viz. Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhi, Staphylococcus aureus, Staphylococcus pyogenus, Bacillus subtilis and antifungal activity against Candida albicans, Aspergillus niger, Aspergillus clavatus strains. © 2011 Elsevier Masson SAS. All rights reserved.


Anuj S.A.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science | Ishnava K.B.,Ashok and Rita Patel Institute of Integrated Study and Research in Biotechnology and Allied science
International Journal of Pharma and Bio Sciences | Year: 2013

It is a well-known fact that silver nanoparticles are highly toxic to microorganisms. So, nowadays the synthesis and antimicrobial activity of silver nanoparticles is a major area of research in the field of nanobiotechnology. The problem with most of the chemical and physical methods of silver nanoparticles is that they are expensive and can also involve the use of toxic, hazardous chemicals, which may pose biological and environmental risks. To overcome this, the biological method provides a feasible alternative. But a major drawback of using bacteria, algae and fungi to synthesize silver nanoparticles is that it is very slow and the cell culture maintaining process when in comparison with plant extracts. Hence, the use of plant materials to synthesize silver nanoparticles becomes an option that is feasible. The Tinospora cordifolia is an important medicinal plant. Recently, this plant also used in the synthesis of silver nanoparticles. In this present study, we have synthesized silver nanoparticles using Tinospora cordifolia dried stem powder from 1mM aqueous silver nitrate. Utilizing the reduced property of stem powder, silver nanoparticles were synthesized at room temperature. The stem powder extracts mixed with silver nitrate showed a gradual change in the color of the extracts from yellow to dark brown. The formation of silver nanoparticles was confirmed by UV-Visible spectrophotometer, X-Ray diffraction (XRD), Fourier transform infrared (FTIR), Energy dispersive spectroscopy (EDAX) and Transmission electron microscopy (TEM). The antibacterial activity of silver nanoparticles against antibiotic resistant bacteria is very important characteristic of silver nanoparticles. When we compare the silver nanoparticles with the antibiotics, it provides an idea about the efficiency of silver nanoparticles.

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