Bajpai B.,Ashok And Rita Patel Institute Of Integrated Study And Research In Biotech And Allied Science Aribas |
Chaudhary M.,Banasthali University |
Chaudhary M.,Apt Therapeutics, Inc. |
Saxena J.,Bt Kumaon Institute Of Technology
Food Technology and Biotechnology | Year: 2015
Haloarchaea are found at very high concentrations in salt-conditioned environments, hence produce enzymes which are able to catalyze reactions under harsh conditions, typical of many industrial processes. In the present study, culture conditions for extracellular amylase production from Haloarchaea isolated from a solar saltern were optimized and the purified enzyme was characterized. Haloferax sp. HA10 showed maximum amylase production at 3 M NaCl, 37 °C, pH=7 and 1 % starch content. Purified α-amylase was a calcium- dependent enzyme with an estimated molecular mass of about 66 kDa and many industrially useful properties. It was found to be stable in a broad range of pH (from 5 to 9) and NaCl concentrations (from 0.5 to 3.0 M), retaining 48 % activity even at 4 M. The optimal temperature for Haloferax sp. HA10 amylase activity was 55 °C (99 % activity), and 57 % activity was retained at 80 °C, which dropped to 44 % with the increase of temperature to 90 or 100 °C. It was able to sustain various surfactants and detergents. To the best of our knowledge the detergent-stable α-amylases from halophilic archaeon have not been reported yet.
Raj M.M.,Institute of Science and Technology for Advanced Studies and Research ISTAR |
Patel H.V.,Ashok And Rita Patel Institute Of Integrated Study And Research In Biotech And Allied Science Aribas |
Raj L.M.,P.A. College |
Patel N.K.,Sardar Patel University
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2013
Novel Thiadiazoles were synthesized from 4-hydroxybenzoic acid and thiosemicarbazide.From these compounds various derivatives of 1,3,4-Thiadiazole derivatives (C1-C5) have been synthesized. The chemical structures of the synthesized compounds were confirmed by means of IR, 1H NMR, and nitrogen estimation. These compounds were screened for antibacterial (Staphylococcus aureus ATCC 9144, Becillus Cereus ATCC 11778, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 2853 and anti-fungal (Aspergillus niger ATCC 9029 and Aspergillus fumigatus ATCC 46645) by paper disc diffusion technique.
Patel D.Y.,Ashok And Rita Patel Institute Of Integrated Study And Research In Biotech And Allied Science Aribas |
Patel R.K.,Ashok And Rita Patel Institute Of Integrated Study And Research In Biotech And Allied Science Aribas
Current Trends in Biotechnology and Pharmacy | Year: 2012
Total 30 French mini straws (0.25ml) of frozen semen of HF and HF crossbred cattle bulls were randomly collected from one of the frozen semen banks for evaluation of microbial load using the standard plate count (SPC) method using nutrient agar plate. These plates were incubated at 37°C for 72 hrs and examined for growth. The average colony count was calculated and bacteria were also identified as Gram positive and Gram negative. A total of 10 biochemical tests were performed to characterize the isolates. Antibiotic sensitivity test was also performed to test the sensitivity against Ampicillin, Erythromycin, Gentamycin and Spectinomycin. The results indicate that eleven samples out of 30 (36.6%) were found positive for various bacterial isolates. In most samples the microbial load was found below the Bureau of Indian Standard (BIS). However one sample (3.33%) was found with fungal infection. Both the Gram positive and Gram negative bacteria were found in these samples. Results of biochemical properties of bacterial isolates are summarized. Each isolates are varying in their biochemical profile. The results of antibiotic sensitivity pattern in bacterial isolates were also summarized. All bacterial isolates exhibited variable pattern against Ampicillin, Erythromycin, Gentamycin and Spectinomycin. The concentrations of antibiotics were 10μg, 10μg, 10μg and 100 μg respectively. Among all these antibiotics all bacterial isolates are resistance against Ampicillin. The article describes detailed investigation of microbial load in frozen semen of HF and HF crossbred cattle bulls.