Yoshida S.,Asahikawa Medical College
Biological Chemistry | Year: 2010
Klk8 is a tryptic serine protease with limited substrate specificity. Klk8 mRNA is expressed in many developing organs, whereas its expression is confined to limited regions, including the hippocampus, in adults. In the hippocampus, Klk8 is involved in activity-dependent synaptic changes such as long-term potentiation, which was found to be suppressed in Klk8 knockout (KO) mice. Oligodendrocytes only expressed Klk8 mRNA after injury to the central nervous system. The epidermis of the skin is one of the tissues that exhibits a high level of KLK8 expression. Klk8 might be involved in desquamation through the degradation of adhesive molecules that connect layers of the epidermis. Klk8 might thus be involved in tissue development and rearrangement. Copyright © 2010 by Walter de Gruyter Berlin New York.
Hosaka M.,Gunma University |
Watanabe T.,Asahikawa Medical College
Endocrine Journal | Year: 2010
Secretory granules in endocrine cells selectively store bioactive peptide hormones and amines, which are secreted in a regulated manner upon appropriate stimulation. In addition to bioactive substances, various proteins and lipids characteristic of secretory granules are likely recruited to a restricted space at the trans-Golgi Network (TGN), and the space then matures to the secretory granule. Although experimental findings so far have strongly suggested that aggregation- and receptor-mediated processes are essential for the formation of secretory granules, the putative link between these two processes remains to be clarified. Recently, secretogranin III (SgIII) has been identified as a specific binding protein for chromogranin A (CgA), a representative constituent of the core aggregate within secretory granules, and it was later revealed that SgIII can also bind to the cholesterol-rich membrane domain at the TGN. Based on its multifaceted binding properties, SgIII may act as a central player in the formation of cholesterol-rich membrane platforms. Upon these platforms, essential processes for secretory granule biogenesis coordinately occur; that is, selective recruitment of prohormones, processing and modifying of prohormones, and condensation of mature hormones as an aggregate. This review summarizes the findings and theoretical concepts on the issue to date and then focuses on the putative role of SgIII in secretory granule biogenesis in endocrine cells.
Takahara M.,Asahikawa Medical College
Advances in Oto-Rhino-Laryngology | Year: 2011
Palmoplantar pustulosis (PPP) is famous for causing typical tonsillar focal diseases. Clinical improvement of PPP rash after tonsillectomy was seen in 109 (94%) of 116 patients by subjective self-assessment, and 52 (88%) of 59 patients by objective Palmoplantar Pustulosis Area and Severity Index (PPPASI) scoring. Flow-cytometric analyses revealed that expression of activation markers (CD25 and HLA) class II increased on tonsillar T cells from PPP and IgAN patients. Moreover, expressions of skin-homing receptors (CLA and CCR6) increased on tonsillar T cells from PPP patients. In addition, the expressions were enhanced by stimulation of indigenous bacteria. Moreover, those expressions also increased in peripheral T cells, and decreased after tonsillectomy. Immunohistchemical analyses revealed that the ligands of those receptors (E-selectin and chemokine ligand 20) were expressed in lesions of skin. On the basis of our findings, migration of tonsillar T cells on which several homing receptors express by hyperimmune response against indigenous bacteria have a critical role in the pathogenesis of tonsillar focal diseases. Copyright © 2011 S. Karger AG, Basel.
Asahikawa Medical College and Fuso Pharmaceutical Industries Ltd. | Date: 2011-04-15
Disclosed is the novel hCL-K1 polypeptide which offers collectin activity. This polypeptide consists of consecutive 271 amino acids set out in SEQ ID NO: 2 and does not bind to both maltose and N-acetylgalactosamine.
Asahikawa Medical College and TSUMURA & Co. | Date: 2015-09-25
It is an object of the invention to discover a substance that effectively increases the production of adrenomedullin, as well as to provide an adrenomedullin production-enhancing agent utilizing this substance. The adrenomedullin production-enhancing agent is characterized by inclusion of a ginsenoside, a sanshool, and/or a shogaol as active ingredients.