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Nishi-Tokyo-shi, Japan

Teranishi S.,Kanagawa University | Cho W.,Kanagawa University | Iwamoto S.,Kanagawa University | Makino Y.,Daikin | And 2 more authors.
Journal of Environmental Engineering (Japan) | Year: 2015

In this study, as the prerequisites for countermeasure of the dew condensation, in order to identify locations and frequency of dew condensation, the estimation of dew condensation on all wall and window in commercial buildings was conducted by TRNSYS simulation coupled with TRNFLOW. The airtightness and the thermal insulation property affect on dew condensation, accordingly the dew condensation risk evaluation with different airtightness and different thermal insulation property was performed. The dew condensation risk was calculated by using dew condensation risk evaluation index proposed by the authors in previous studies.

Kawamoto K.,Kawamoto Engineering | Cho W.,Kanagawa University | Kohno H.,Asahi Kogyosha Co. | Koganei M.,Yamaguchi University | And 2 more authors.
Energies | Year: 2016

A desiccant air-conditioning system was developed as a latent-load-processing air conditioner in a dedicated outdoor air system during the summer. This study investigated the application of this air-conditioning system to humidification during the winter without using make-up water, thereby eliminating the cause of microbial contamination in air-conditioning systems. The experiments were conducted with a system used for summer applications to determine the feasibility of adsorbing vapor from outdoor air and supplying it to an indoor space. The humidification performance, energy efficiency, and operating conditions were examined. Although the conditions were subpar because the experiments were performed with an actual dedicated outdoor air system, the results showed that it is possible to supply air with a minimum humidity ratio of 5.8 g/kg dry air (DA) when the humidity ratio of outdoor air ranges from 1.8 to 2.3 g/kg DA. The minimum humidification performance required for a dedicated outdoor air system was achieved by increasing the airflow rate of the moisture-adsorption side to 2-3 times that of the humidification side. In addition, air leaking from the moisture-adsorption side to the humidification side, improving the mechanical structure, such as by the insulation of the moisture-adsorption side, and an efficient operating method were examined for humidification during the winter. © 2016 by the authors.

Shoda N.,Asahi Kogyosha Co. | Kohno H.,Asahi Kogyosha Co. | Koganei M.,Yamaguchi University | Iwamoto S.,Kanagawa University | And 3 more authors.
ICOPE 2015 - International Conference on Power Engineering | Year: 2015

In this study, a desiccant outdoor air-conditioning system that uses high-temperature chilled water (approximately 20 °C) and low-temperature hot water (approximately 55 °C) produced by a high-efficiency heat pump, and hot air produced by solar heat collectors, was proposed. This system treats the total heat load of outdoor air and indoor latent heat load in commercial buildings. In the proposed system, solar heat is effectively used for regeneration of the desiccant in summer and for pre-heating of ventilation air in winter. We have constructed a prototype of this system and evaluated the coefficient of performance (COP) of the entire desiccant air-conditioning system using the measured values of electric energy consumption and treated enthalpy between the outdoor air and supply air.

Kashima K.,Tokyo Medical University | Kashima K.,Asahi Kogyosha Co. | Mejima M.,Tokyo Medical University | Kurokawa S.,Tokyo Medical University | And 5 more authors.
BMC Genomics | Year: 2015

Background: We have developed a rice-based oral cholera vaccine named MucoRice-CTB (Cholera Toxin B-subunit) by using an Agrobacterium tumefaciens-mediated co-transformation system. To assess the genome-wide effects of this system on the rice genome, we compared the genomes of three selection marker-free MucoRice-CTB lines with those of two wild-type rice lines (Oryza sativa L. cv. Nipponbare). Mutation profiles of the transgenic and wild-type genomes were examined by next-generation sequencing (NGS). Results: Using paired-end short-read sequencing, a total of more than 300 million reads for each line were obtained and mapped onto the rice reference genome. The number and distribution of variants were similar in all five lines: the numbers of line-specific variants ranged from 524 to 842 and corresponding mutation rates ranged from 1.41×10-6 per site to 2.28×10-6 per site. The frequency of guanine-to-thymine and cytosine-to-adenine transversions was higher in MucoRice-CTB lines than in WT lines. The transition-to-transversion ratio was 1.12 in MucoRice-CTB lines and 1.65 in WT lines. Analysis of variant-sharing profiles showed that the variants common to all five lines were the most abundant, and the numbers of line-specific variant for all lines were similar. The numbers of non-synonymous amino acid substitutions in MucoRice-CTB lines (15 to 21) were slightly higher than those in WT lines (7 or 8), whereas the numbers of frame shifts were similar in all five lines. Conclusions: We conclude that MucoRice-CTB and WT are almost identical at the genomic level and that genome-wide effects caused by the Agrobacterium-mediated transformation system for marker-free MucoRice-CTB lines were slight. The comparative whole-genome analyses between MucoRice-CTB and WT lines using NGS provides a reliable estimate of genome-wide differences. A similar approach may be applicable to other transgenic rice plants generated by using this Agrobacterium-mediated transformation system. © 2014 Kashima et al.

Kashima K.,Tokyo Medical University | Kashima K.,Asahi Kogyosha Co. | Yuki Y.,Tokyo Medical University | Yuki Y.,Tokyo International University | And 18 more authors.
Plant Cell Reports | Year: 2016

Key message: The first Good Manufacturing Practices production of a purification-free rice-based oral cholera vaccine (MucoRice-CTB) from transgenic plants in a closed cultivation system yielded a product meeting regulatory requirements. Abstract: Despite our knowledge of their advantages, plant-based vaccines remain unavailable for human use in both developing and industrialized countries. A leading, practical obstacle to their widespread use is producing plant-based vaccines that meet governmental regulatory requirements. Here, we report the first production according to current Good Manufacturing Practices of a rice-based vaccine, the cholera vaccine MucoRice-CTB, at an academic institution. To this end, we established specifications and methods for the master seed bank (MSB) of MucoRice-CTB, which was previously generated as a selection-marker-free line, evaluated its propagation, and given that the stored seeds must be renewed periodically. The production of MucoRice-CTB incorporated a closed hydroponic system for cultivating the transgenic plants, to minimize variations in expression and quality during vaccine manufacture. This type of molecular farming factory can be operated year-round, generating three harvests annually, and is cost- and production-effective. Rice was polished to a ratio of 95 % and then powdered to produce the MucoRice-CTB drug substance, and the identity, potency, and safety of the MucoRice-CTB product met pre-established release requirements. The formulation of MucoRice-CTB made by fine-powdering of drug substance and packaged in an aluminum pouch is being evaluated in a physician-initiated phase I study. © 2015, Springer-Verlag Berlin Heidelberg.

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