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Rajak S.K.,National Dairy Research Institute | Kumaresan A.,National Dairy Research Institute | Gaurav M.K.,National Dairy Research Institute | Muhammad Aslam M.K.,National Dairy Research Institute | And 6 more authors.
Indian Journal of Animal Sciences | Year: 2013

Testicular fine needle aspiration cytology (FNAC) is widely being used in humans to evaluate the functional testicular mechanism, but its application is very limited in animals especially breeding bulls. This preliminary study was conducted to assess the effect of testicular fine needle aspiration (FNA) technique on testicular biometry and seminal characteristics in Karan Fries crossbred bulls. Eight bulls within the age range of 4-6 years were utilized for study. Before and after fine needle aspiration, testicular parameters were measured and seminal quality was assessed as per standard protocols. There was no significant difference observed in scrotal circumference and testicular length before and after the treatment. The testicular width was 6.59±0.32 and 7.08±0.27 during pre- and post-aspiration, while the corresponding values for testicular mass were 355.77±39.20 and 427±28.75, respectively. There was no significant changes observed in ejaculate volume, mass activity and individual motility in bulls during pre-FNA and post-FNA period. Similarly, the percentage of live spermatozoa, membrane intact spermatozoa and acrosome intact spermatozoa did not differ significantly between pre-FNA and post-FNA period. These results indicated that testicular fine needle aspiration technique can be used as a routine diagnostic method to detect sub-fertility and infertility in crossbred bulls without affecting their reproductive health.


Sabhapati M.,National Dairy Research Institute | Raina V.S.,National Dairy Research Institute | Bhakat M.,National Dairy Research Institute | Mohanty T.K.,National Dairy Research Institute | And 4 more authors.
Indian Journal of Animal Sciences | Year: 2016

Sub-fertility is one of the major concerns in crossbred bulls as 42.98% bulls are getting culled due to sub-fertility problems therefore, a study was planned on 18 Karan Fries bulls producing poor quality semen or not mounted. They were randomly grouped into 3 treatment groups of 6 animals each and 2 normal bulls were kept as control. Sexual behavior and semen quality were studied during pre-therapy (1 month), therapy (90 days) and post-therapy (90 days) period. Data were analyzed using ANOVA. During therapy period, first group treated with 10 g of herbal product orally, second group with 0.0082 mg of GnRH intramuscularly at 10 days interval and third group with 80 ppm of zinc for 90 days for each group. The results depicted that herbal and GnRH treatment significantly reduced the reaction time, whereas herbal, GnRH treatment and zinc supplementation significantly improved the libido score and reduced per cent of non-mounts during the therapy and post-therapy period. The volume of semen and level of testosterone improved in all treatments groups. Herbal, GnRH treatment and zinc supplementation increased the average number of good and medium quality ejaculates/month/bull and maximum efficiency achieved during the treatment period. There was increase in percent live sperm and decrease in abnormality in herbal treated and zinc supplemented group. Therefore, it can be concluded that GnRH therapy, herbal treatment and zinc supplementation will be effective to overcome the sub-fertility problem in crossbred bulls. © 2016, Indian Council of Agricultural Research. All rights reserved.


Vijetha B.T.,National Dairy Research Institute | Rajak S.K.,National Dairy Research Institute | Layek S.S.,National Dairy Research Institute | Kumaresan A.,National Dairy Research Institute | And 8 more authors.
Indian Journal of Animal Sciences | Year: 2014

The present study analyzed the relationship between testicular biometry and initial semen quality in Karan Fries (KF) crossbred bulls. Data on semen production traits of KF bulls (n=32) spread over a period of 3 years were analyzed to find out the ejaculate rejection rate (ERR). The scrotal circumference (SC), testicular length (TL) and testicular width (TW) were measured once in a month and 16 ejaculates were collected from bulls (n=24). The relationship between different testicular measurements and the ejaculate quality was studied. To study if the testicular biometry parameters differ between good and poor semen producing bulls, based on the semen evaluation, the bulls were ranked into good and poor. Our study revealed that ERR in KF bulls varied from 23.02 to 100%, with the average of 52.46%. The SC was significantly negatively correlated (-0.459) with ERR. Between good and poor bulls, there was no difference in SC, TL and TW, however, testicular mass (TM) and testicular volume (TV) differed significantly. From the results of the study, it may be inferred that that SC had a positive relationship with initial semen quality, but combination of SC, TL, TW, TV and TM may yield superior results for the selection of crossbred bulls.


Singh A.P.,National Dairy Research Institute | Singh A.P.,Artificial Breeding Research Center | Singh R.,National Dairy Research Institute | Singh R.,Bhai Patel University of Agriculture science | And 6 more authors.
Indian Journal of Animal Sciences | Year: 2013

Healthy breeding bulls (12) were selected to find out the effect of mist cooling and fanning treatment in reducing the higher ambient temperature stress from animals and help them to regain their normal status. All bulls were uniformly divided into 2 groups. Sexual behaviour and semen characteristics (ejaculate volume, sperm viability, membrane integrity, individual motility) were recorded. A significant improvement was observed in sexual behaviour (DMT, ES, PS, ITS and libido) of bulls under mist cooling and fanning. However, no significant effect was observed in their TS, RS and Flehmen's Reaction. Mist cooling treatment also exerted positive effects on their semen characteristics by improving significantly their motility, viability, membrane integrity and post thaw motility whereas no significant effect on semen volume and colour.

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