Sanchez M.,Arthroscopic Surgery Unit Hospital Vithas San Jose Vitoria Gasteiz Spain |
Anitua E.,Eduardo Anitua Foundation for Biomedical Research Vitoria Gasteiz Spain |
Delgado D.,Arthroscopic Surgery Unit Research Hospital Vithas San Jose Vitoria Gasteiz Spain |
Prado R.,Biotechnology Institute BTI Vitoria Gasteiz Spain |
And 9 more authors.
Journal of Tissue Engineering and Regenerative Medicine | Year: 2015
In the present study we evaluated the motor recovery process of peripheral nerve injury (PNI), based on electrophysiological and histomorphometric criteria, after treatment with plasma rich in growth factors (PRGF) injections and scaffolds in an ovine model. Three groups of sheep underwent a nerve crush lesion: the first group (n=3) was left to recover spontaneously (SR); the second group was administered saline injections (SI; n=5) and a third group (n=6) received PRGF injections and scaffolds immediately after the crush injury. At post-intervention week 8, 70% of sheep in the PRGF group were CMAP-positive, with no electrophysiological response in the rest of the groups. Histomorphometric analysis 12weeks after the surgical intervention revealed that the average axonal density of the SR (1184±864 axons/μm2) and SI (3109±2450 axons/μm2) groups was significantly inferior to the control (8427±2433 axons/μm2) and also inferior to the PRGF group (5276±4148 axons/μm2), showing no significant differences between the control and PRGF groups. The axonal size of the SR and SI groups was significantly smaller compared with the control group (18±4μm2), whereas the axonal size of the PRGF group (6±5μm2) did not show statistical differences from the control. Morphometry of the target muscles indicated that the PRGF group had the lowest percentage volume reduction 12weeks after the crush injury. The PRGF group had larger muscle fibre areas than the SI and SR groups, although the differences did not reach statistical significance. Overall, these data suggest that the PRGF injections and scaffolds hastened functional axon recovery and dampened atrophy of the target muscles in an ovine model. © 2015 John Wiley & Sons, Ltd.