Ma G.,ART Advanced Research Technologies Inc.
ACS Applied Materials and Interfaces | Year: 2013
The interest in optical molecular imaging of small animals in vivo has been steadily increased in the last two decades as it is being adopted by not only academic laboratories but also the biotechnical and pharmaceutical industries. In this Spotlight paper, the elements for in vivo optical molecular imaging are briefly reviewed, including contrast agents, i.e., various fluorescent reporters, and the most commonly used technologies to detect the reporters. The challenges particularly for in vivo fluorescence imaging are discussed and solutions to overcome the said-challenges are presented. An advanced imaging technique, in vivo fluorescence lifetime imaging, is introduced together with a few application examples. Taking advantage of the long fluorescence lifetime of quantum dots, a method to achieve background-free in vivo fluorescence small animal imaging is demonstrated. © 2013 American Chemical Society.
Softscan Healthcare Group Ltd., Art Advanced Research Technologies Inc., Alerion Biomedical Inc. and MetaProbe Inc. | Date: 2005-12-06
Art Advanced Research Technologies Inc. | Date: 2011-10-11
The present invention relates to a method of decomposition of a test sample into constituents thereof. The method proceeds by optically imaging the test sample to obtain a corresponding unknown time-domain resolved signal and decomposes the unknown time-domain resolved signal by comparing the unknown time-domain resolved signal with time-domain resolved reference signals. Furthermore, the method allows the determination of the presence or absence of constituents. Relative quantities may also be determined if sample material properties are known or taken into account. Lifetime decay of the constituents may also be estimated by handling effect of light diffusion in the test sample as time decay.
Wyatt S.K.,Vanderbilt University |
Hcharles M.,Vanderbilt University |
Bai M.,Vanderbilt University |
Bailey S.N.,Vanderbilt University |
And 4 more authors.
Molecular Imaging and Biology | Year: 2010
Purpose: To quantitatively evaluate the utility of a translocator protein (TSPO)-targeted near-infrared (NIR) probe (NIR-conPK11195) for in vivo molecular imaging of TSPO in breast cancer. Procedures: NIR-conPK11195 uptake and TSPO-specificity were validated in TSPO-expressing human breast adenocarcinoma cells (MDA-MB-231). In vivo NIR-conPK11195 biodistribution and accumulation were quantitatively evaluated in athymic nude mice bearing MDA-MB-231 xenografts. Results: Fluorescence micrographs illustrated intracellular labeling of MDA-MB-231 cells by NIR-conPK11195. Quantitative uptake and competition assays demonstrated dose-dependent (p<0.001) and TSPO-specific (p<0.001) NIR-conPK11195 uptake. In vivo, NIR-conPK11195 preferentially labeled MDA-MB-231 tumors with an 11-fold (p<0.001) and 7-fold (p<0.001) contrast enhancement over normal tissue and unconjugated NIR dye, respectively. Conclusions: NIR-conPK11195 appears to be a promising TSPO-targeted molecular imaging agent for visualization and quantification of breast cancer cells in vivo. This research represents the first study to demonstrate the feasibility of TSPO imaging as an alternative breast cancer imaging approach. © Academy of Molecular Imaging, 2009.
Van De Ven S.M.W.Y.,Stanford University |
Mincu N.,ART Advanced Research Technologies Inc. |
Brunette J.,ART Advanced Research Technologies Inc. |
Ma G.,ART Advanced Research Technologies Inc. |
And 3 more authors.
Molecular Imaging and Biology | Year: 2011
Purpose: The aim of the study was to determine the feasibility of using a clinical optical breast scanner with molecular imaging strategies based on modulating light transmission. Procedures: Different concentrations of single-walled carbon nanotubes (SWNT; 0.8-20.0 nM) and black hole quencher-3 (BHQ-3; 2.0-32.0 μM) were studied in specifically designed phantoms (200-1,570 mm 3) with a clinical optical breast scanner using four wavelengths. Each phantom was placed in the scanner tank filled with optical matching medium. Background scans were compared to absorption scans, and reproducibility was assessed. Results: All SWNT phantoms were detected at four wavelengths, with best results at 684 nm. Higher concentrations (≥8.0 μM) were needed for BHQ-3 detection, with the largest contrast at 684 nm. The optical absorption signal was dependent on phantom size and concentration. Reproducibility was excellent (intraclass correlation 0.93-0.98). Conclusion: Nanomolar concentrations of SWNT and micromolar concentrations of BHQ-3 in phantoms were reproducibly detected, showing the potential of light absorbers, with appropriate targeting ligands, as molecular imaging agents for clinical optical breast imaging. © Academy of Molecular Imaging and Society for Molecular Imaging, 2010.