Kunming Army General Hospital

Kunming, China

Kunming Army General Hospital

Kunming, China
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Zhu X.-Q.,Kunming Army General Hospital | Pan X.-H.,Kunming Army General Hospital | Yao L.,Stanford University | Li W.,Jilin University | And 7 more authors.
Journal of Cellular Biochemistry | Year: 2016

Transplantation of hepatocytes is a promising therapy for end-stage liver disease, but the availability of functional cells currently precludes its clinical application. We now report a simple transient reprogramming approach to convert fibroblasts into hepatic-like cells. Human skin fibroblasts were treated with fish egg extracts to become the transiently remodeled cells (TRCs). After infected with retroviral EGFP, they were directly injected into the fetal monkey liver, where they underwent in situ differentiation in the hepatic niche. The hepatic-like cells were functional as shown by the synthesis of hepatic markers in vivo, including albumin, cytokeratin-18, and hepatic serum antigen. Similarly, when implanted in the mouse liver, the TRCs were differentiated into hepatic-like cells that synthesize albumin and CK18 and became completely integrated into the liver parenchyma. The potency of TRCs was mechanistically related to the activation of several signal pathways, which reactivate endogenous genes related to cell potency. This study demonstrates the feasibility of a simple and inexpensive epigenetic remodeling approach to convert human fibroblasts into therapeutic hepatic-like cells for the treatment of end-stage liver disease. © 2015 Wiley Periodicals, Inc.


PubMed | Jilin University, Stanford University, Kunming Army General Hospital and GMR Epigenetics
Type: Journal Article | Journal: Journal of cellular biochemistry | Year: 2016

Transplantation of hepatocytes is a promising therapy for end-stage liver disease, but the availability of functional cells currently precludes its clinical application. We now report a simple transient reprogramming approach to convert fibroblasts into hepatic-like cells. Human skin fibroblasts were treated with fish egg extracts to become the transiently remodeled cells (TRCs). After infected with retroviral EGFP, they were directly injected into the fetal monkey liver, where they underwent in situ differentiation in the hepatic niche. The hepatic-like cells were functional as shown by the synthesis of hepatic markers in vivo, including albumin, cytokeratin-18, and hepatic serum antigen. Similarly, when implanted in the mouse liver, the TRCs were differentiated into hepatic-like cells that synthesize albumin and CK18 and became completely integrated into the liver parenchyma. The potency of TRCs was mechanistically related to the activation of several signal pathways, which reactivate endogenous genes related to cell potency. This study demonstrates the feasibility of a simple and inexpensive epigenetic remodeling approach to convert human fibroblasts into therapeutic hepatic-like cells for the treatment of end-stage liver disease.


Zhu X.-Q.,Kunming University of Science and Technology | Zhu X.-Q.,Kunming Army General Hospital | Lei L.,Kunming Army General Hospital | Wu Q.,Kunming Army General Hospital | And 6 more authors.
Journal of Animal and Veterinary Advances | Year: 2013

The treatment of bone defect is the multi-disciplinary clinical problem. Although, several types of bone graft materials are used for clinical study, numerous hurdles such as infection and immune rejection need to be resolved in essence. Various types of stem cells combining with biological material for repairing bone defect is a promising way. However, the poor resource of stem cells has restricted their clinical application. In earlier research, researchers had reported that transient in vitro epigenetic reprogramming of mouse skin fibroblasts into multipotent stem cells. In this study, researchers used the similar method to reprogram dog skin fibroblasts into multipotent stem cells. Further, reprogrammed cells were re-differentiated into osteogenesis-like cells in vitro. Especially, iMS cells combining with Hydroxyapatite/β-Tricalcium Phosphate/Chitosan (HAP/β-TCP/CS) Composite Scaffold could repair bone defect in dog. The results indicated that the iMS could be used as seed cell for repairing bone defect in clinical application. © Medwell Journals, 2003.


Zhu X.,Kunming University of Science and Technology | Zhu X.,Kunming Army General Hospital | Cai X.,Kunming Army General Hospital | Chen Q.,Kunming University of Science and Technology | Pan X.,Kunming Army General Hospital
Research Journal of Medical Sciences | Year: 2015

The shortage of bone marrow donors and the high risk of allogenic transplant rejection have prompted investigation into alternative therapeutic approaches for patients with aplastic anemia. Reconstitution of hematopoietic function using stem cells derived from cord blood is a promising alternative which however, the limited number of stem cells obtained restricts clinical application. In the earlier study, researchers demonstrated the epigenetic reprogramming of mouse skin fibroblasts into multipotent cells using a transient in vitro method. After transient reprogramming, cells expressed reprogramming factors (Oct-3/4, Nanog and Sox-2) and formed teratomas in nude mice. In this study, PBMCs from aplastic anemia patients were reprogrammed using the transient in vitro method. Reprogrammed cells (iMS cells) were capable of differentiating into hematopoietic cells; iMS cells transplanted into lethally irradiated mice differentiated into bone marrow and splenic cells. Results indicate that reprogrammed PBMCs are multipotent and can restore hematopoietic function. © Medwell Journals, 2013.


Wu Q.,Northeast Normal University | Wu Q.,Kunming University | Wu Q.,Kunming Army General Hospital | Hao X.,Northeast Normal University | And 6 more authors.
Inorganic Chemistry Communications | Year: 2012

A new organic-inorganic hybrid compound (NH 4) 2[{Mn(salen)(H 2O)} 6V 6O 18](NO 3) 2·30H 2O (salen 2- = N,N′-(ethylene)bis(salicylideneiminate)) (1) was synthesized by the reaction between NH 4VO 3 and [Mn(salen)(H 2O) 2]ClO 4 in a methanol-water solution with pH = 5.0 adjusted by nitric acid. Compound 1 was characterized by elemental analyses, TG, IR, UV-vis, XPS and the single-crystal X-ray diffraction. Compound 1 possesses a discrete cyclic hexanuclear vanadates, which is unprecedentedly grafted by six [Mn(salen)(H 2O) 2] units. Such a new hybrid compound displays good UV photocatalytic activity for RhB degradation. © 2012 Elsevier B.V. All rights reserved.


Zhu X.-Q.,Kunming Army General Hospital | Pan X.-H.,Kunming Army General Hospital | Wang W.,Stanford University | Chen Q.,Kunming Army General Hospital | And 4 more authors.
Biomaterials | Year: 2010

Multipotent stem cells have the potential to establish a new field of promising regenerative medicine to treat tissue damage, genetic disorders, and degenerative diseases. However, limited resource of stem cells has turned to be an evitable obstacle in clinical applications. We utilized a simple in vitro epigenetic reprogramming approach to convert skin fibroblasts into multipotent cells. After transient reprogramming, stem cell markers, including Oct4 and Nanog, became activated in the treated cells. The reprogrammed cells were multipotent as demonstrated by their ability to differentiate into a variety of cells and to form teratomas. Genomic imprinting of insulin-like growth factor II (Igf2) and H19 was not affected by this short period of cell reprogramming. This study may provide an alternative strategy to efficiently generate patient-specific stem cells for basic and clinical research, solving major hurdles of virally-induced pluripotent stem (iPS) cells that entail the potential risks of mutation, gene instability, and malignancy. © 2009 Elsevier Ltd. All rights reserved.


PubMed | Kunming Army General Hospital
Type: Journal Article | Journal: Biomaterials | Year: 2010

Multipotent stem cells have the potential to establish a new field of promising regenerative medicine to treat tissue damage, genetic disorders, and degenerative diseases. However, limited resource of stem cells has turned to be an evitable obstacle in clinical applications. We utilized a simple in vitro epigenetic reprogramming approach to convert skin fibroblasts into multipotent cells. After transient reprogramming, stem cell markers, including Oct4 and Nanog, became activated in the treated cells. The reprogrammed cells were multipotent as demonstrated by their ability to differentiate into a variety of cells and to form teratomas. Genomic imprinting of insulin-like growth factor II (Igf2) and H19 was not affected by this short period of cell reprogramming. This study may provide an alternative strategy to efficiently generate patient-specific stem cells for basic and clinical research, solving major hurdles of virally-induced pluripotent stem (iPS) cells that entail the potential risks of mutation, gene instability, and malignancy.

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