Armed Police Hospital of Shanghai
Armed Police Hospital of Shanghai
Wang F.,Tongji University |
Ma H.,Tongji University |
Liu Z.,Nantong University |
Huang W.,Tongji University |
And 2 more authors.
Biomedicine and Pharmacotherapy | Year: 2017
Skin cancer is the most common form of cancer responsible for considerable morbidity and mortality, the treatment progress of which remains slow though. Therefore, studies identifying anti-skin cancer agents that are innocuous are urgently needed. α-Mangostin, a natural product isolated from the pericarp of mangosteen fruit, has potent anti-cancer activity. However, its role in skin cancer remains unclear. The aim of this study was to evaluate the treatment effect of α-mangostin on skin tumorigenesis induced by 9,10-dimethylbenz[a]anthracene (DMBA)/TPA in mice and the potential mechanism. Treatment with α-mangostin significantly suppressed tumor formation and growth, and markedly reduced the incidence rate. α-Mangostin not only inhibited the expressions of pro-inflammatory factors, but also promoted the production of anti-inflammatory factors in tumor and blood. It induced autophagy of skin tumor and regulated the expressions of autophagy-related proteins. The protein expressions of LC3, LC3-II and Beclin1 increased whereas those of LC3-I and p62 decreased after treatment with α-mangostin. Moreover, α-mangostin promoted the apoptosis of skin tumor dose-dependently by up-regulating of Bax, cleaved caspase-3, cleaved PARP and Bad, and down-regulating of Bcl-2 and Bcl-xl. Furthermore, showed α-mangostin inhibited the PI3K/AKT/mTOR (mammalian target of rapamycin) signaling pathway, as evidenced by decreased expressions of phospho-PI3K (p-PI3K), p-Akt and p-mTOR, but did not affect the expressions of t-PI3K, t-Akt or t-mTOR. Collectively, α-mangostin suppressed murine skin tumorigenesis induced by DMBA/TPA through inhibiting inflammation and promoting autophagy and apoptosis by regulating the PI3K/Akt/mTOR signaling pathway, as a potential candidate for future clinical therapy. © 2017 Elsevier Masson SAS
Liu H.,Shanghai JiaoTong University |
Liu H.,CAS Shanghai Institutes for Biological Sciences |
Yang E.,Shanghai JiaoTong University |
Lu X.,Armed Police Hospital of Shanghai |
And 9 more authors.
Pulmonary Pharmacology and Therapeutics | Year: 2015
Pulmonary hypertension (PH) is a rapidly progressive disease that eventually leads to right heart failure and death. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and its receptors (TRAIL-Rs) play an important role in the survival, migration, and proliferation of vascular smooth muscle cells. However, the association between serum TRAIL levels and PH is unknown. In this study, we assayed the serum soluble TRAIL (sTRAIL) levels in 78 patients with PH and 80 controls. The sTRAIL concentrations were elevated in the PH patients compared with the controls (138.76±6.60pg/mL vs. 80.14±3.38pg/mL, p<0.0001). The presence of sTRAIL levels of >103pg/mL could discriminate PH patients from healthy individuals, with a sensitivity of 75.6% and specificity of 81.2%. Moreover, elevated sTRAIL concentrations were associated with eventual pathological complications; this is consistent with the finding that sTRAIL levels decreased in patients who responded to treatment. In a hypoxia-induced PH mouse model, sTRAIL levels were significantly higher compared with those in normoxia mice, and clearly decreased when the mice were treated with treprostinil. The sTRAIL levels were positively correlated with right ventricular systolic pressure and the index of right ventricular hypertrophy. In conclusion, serum sTRAIL could be a biomarker for diagnosis and effective therapy for PH patients. © 2015 Elsevier Ltd.
Song J.,Shanghai JiaoTong University |
Song J.,Armed Police Hospital of Shanghai |
Zhu L.,Shanghai JiaoTong University |
Li X.,Shanghai JiaoTong University
Chinese Journal of Gastroenterology | Year: 2014
Background: Prognosis of early colorectal carcinoma (ECC) is well because of low rate of lymph-node metastasis (LNM). How to improve the detection rate and select appropriate therapy for ECC has been an eager task in clinical practice. Aims: To analyze the pathological features of ECC treated by endoscopic resection (ER) or surgery, and evaluate the therapeutic efficacy of ER. Methods: Pathological data of 503 ECC lesions treated by ER or surgery were retrospectively analyzed. Risk factors of infiltration depth, LNM of ECC were analyzed, and therapeutic efficacy of ER was evaluated. Results: The overall detection rate of ECC was 10.7%. The incidence of LNM was 1.2% (6/503); the LNM incidence of mucosal high-grade neoplasia was 0% (0/247), while was 2.3% (6/256) in submucosal carcinoma. The LNM incidence of submucosal superficial carcinoma treated by ER was 0% (0/31). Tumor location, size, histological type and LNM had significant impacts on infiltration depth (P<0.05). The rates of en bloc, complete and curative resection by ER were 96.0%, 94.2% and 82.1%, respectively. Infiltration depth was the risk factor of piecemeal, incomplete and noncurative resection of ER (P<0.05). Conclusions: The incidence of LNM in ECC is extremely low. Accurate evaluation of character and infiltration depth of lesion before operation is helpful for selecting appropriate therapy. It should be cautious to choose ER for lesions infiltrated into submucosa, thereby to improve the therapeutic efficacy of ER. ©, 2014, Shanghai Institute of Digestive Diseases. All right reserved.
PubMed | CAS Shanghai Institutes for Biological Sciences, Shanghai JiaoTong University and Armed Police Hospital of Shanghai
Type: | Journal: Pulmonary pharmacology & therapeutics | Year: 2015
Pulmonary hypertension (PH) is a rapidly progressive disease that eventually leads to right heart failure and death. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and its receptors (TRAIL-Rs) play an important role in the survival, migration, and proliferation of vascular smooth muscle cells. However, the association between serum TRAIL levels and PH is unknown. In this study, we assayed the serum soluble TRAIL (sTRAIL) levels in 78 patients with PH and 80 controls. The sTRAIL concentrations were elevated in the PH patients compared with the controls (138.766.60pg/mL vs. 80.143.38pg/mL, p<0.0001). The presence of sTRAIL levels of >103pg/mL could discriminate PH patients from healthy individuals, with a sensitivity of 75.6% and specificity of 81.2%. Moreover, elevated sTRAIL concentrations were associated with eventual pathological complications; this is consistent with the finding that sTRAIL levels decreased in patients who responded to treatment. In a hypoxia-induced PH mouse model, sTRAIL levels were significantly higher compared with those in normoxia mice, and clearly decreased when the mice were treated with treprostinil. The sTRAIL levels were positively correlated with right ventricular systolic pressure and the index of right ventricular hypertrophy. In conclusion, serum sTRAIL could be a biomarker for diagnosis and effective therapy for PH patients.
Yang F.-Q.,Tongji University |
Huang J.-H.,Tongji University |
Guo C.-C.,Tongji University |
Luo J.,Tongji University |
And 5 more authors.
Tumor | Year: 2013
Objective: To investigate the effects of VPA (valproate) on proliferation, cell cycle distribution and apoptosis of human kidney carcinoma ACHN cells and the possible underlying mechanisms. Methods: The effect of VPA on the proliferation of ACHN cells was examined by CCK-8 (cell counting kit-8) assay. Flow cytometry was used to analyze the cell cycle distribution and apoptosis of ACHN cells exposed to VPA. The mRNA expressions of cyclin E1, P 21WAF1, Bcl-2 and Bax were detected by real-time fluorescence quantitative-PCR. Results: Incubation with various concentrations of VPA for 48 h resulted in a significant inhibition of proliferation of ACHN cells with an IC50 (50% inhibitory concentration) value of 4.21 mmol/L. After treatment with VPA, the cell cycle was arrested obviously at G 0/G1 phase and the apoptotic rate was significantly increased as compared with the control group. After treatment with 4 mmol/L VPA for 48 h, the levels of P21WAF1 and Bax mRNAs were both significantly increased, and at the same time, the levels of cyclin E1 and Bcl-2 mRNAs were obviously decreased. Conclusion: VPA can inhibit the proliferation of kidney carcinoma ACHN cells by inducing cell-cycle arrest and apoptosis. Copyright © 2013 by TUMOR.
Gong R.,Armed Police Hospital of Anhui |
Sun D.,Armed Police Hospital of Anhui |
Zhong X.,Armed Police Hospital of Anhui |
Sun Y.,Armed Police Hospital of Anhui |
Li L.,Armed Police Hospital of Shanghai
International Journal of Clinical and Experimental Medicine | Year: 2015
Background: MEK1 is overexpressed in various human carcinomas, but the role of MEK1 is not well unknown in hepatocellular carcinoma (HCC). In the present study, we aimed to explore MEK1 expression of in HCC tissues, and to evaluate its relationship with clinical pathological features. Methods: Expressions of MEK1 were detected by western blot assay, real-time quantitative PCR and immunohistochemical (IHC) staining in 30 HCC tissues and their adjacent normal tissues. Pearson Chi-square test was used to analyze the relationship between MEK1 expression and clinical pathological features. The survival curve was drawn by Kaplan-Meier method, and survival was analyzed by Lon-rank test. Results: The expression of MEK1 mRNA in HCC tissues was significantly higher than that in adjacent normal tissues and so was the expression of MEK1 protein. In the 30 specimens, 70% was with Tumor/Normal ratio > 2, 10% with Tumor/Normal ratio < 1 and 20% with 1 < Tumor/Normal ratio < 2. The mean survival time in high MEK1 expression group was significantly lower than that in low MEK1 expression group (Log-rank value = 11.946, P < 0.01). Conclusion: MEK1 expressions in HCC tissues were significantly higher than that in adjacent normal tissues, which indicated that MEK1 was involved in the genesis and development of HCC. Moreover, it was closely related to the postoperative survival time of patients with HCC. © 2015, Int J Clin Exp Med. All rights reserved.
Feng G.,Armed Police Hospital of Shanghai |
Qin D.,Armed Police Hospital of Shanghai |
Jie C.,Armed Police Hospital of Shanghai |
Pei C.,Armed Police Hospital of Shanghai |
Shao-liang W.,Armed Police Hospital of Shanghai
Chinese Journal of Tissue Engineering Research | Year: 2014
BACKGROUND: Related studies have showed that poly D,L-lactide-co-glycolide can effectively package antisense oligonucleotides, small interfering RNA, microRNA. Poly D,L-lactide-co-glycolide can better protect them against the destruction of the enzymes in vivo and have slow the drug release. Therefore, the number of drug administration can be reduced to achieve a long-term and effective therapeutic effect. OBJECTIVE: To prepare poly D,L-lactide -co-glycolide-CXCR4-miRNA-nano-particles and to research the characteristics of the prepared nanoparticles. METHODS: Poly D,L-lactide-co-glycolide-CXCR4-miRNA nanoparticles were prepared by double emulsion-evaporation process. Ultraviolet spectrophotometry was utilized for measurement of encapsulation efficiency and drug-loading rate, observing the shape of nanoparticles by transmission electron microscope, and measuring the size and distribution of nanoparticles by laser particle size analyzer. Sustained-release characteristics of nanoparticle suspension were observed in phosphate buffer. RESULTS AND CONCLUSION: The prepared nanoparticles were spherical-shaped, smooth, evently distributed and inadhesive. The particle size was mainly distributed within 143-502 nm, with an average diameter of 280 nm. The average drug loading was (0.515±0.023)%, the average encapsulation ratio was 50.2% and difference between batches was small. The nanoparticles could slowly release in vitro and the process initially experienced the fast-release stage, and then reached a basically stable platform stage at day 14. These finding indicate that the process to prepare poly D,L-lactide-co-glycolide CXCR4-miRNA-nanoparticles by double emulsion-evaporation is simple. The prepared nanoparticles are well targeted and exhibit sustained-release effects. © 2014, Journal of Clinical Rehabilitative Tissue Engineering Research All Rights Reserved.
Zhou Y.,Armed Police Hospital of Shanghai |
Xu X.-J.,Armed Police Hospital of Shanghai
Journal of Clinical Dermatology | Year: 2014
A 52-year-old man suffered from mandibular skin swelling for 10 days and ulceration for 4 days. Swelling and ulcer with discharges were seen on both sides of the submandibular area. A few tender masses were palpated in the submandibular area Bacterial culture of ulcer discharges showed positive for Klebsiella pneumoniae. Clinical diagnosis: Klebsiella pneumoniae-induced submandibular skin ulcer.