Chen T.-S.,China Medical University at Taichung |
Chang M.-H.,Taichung Armed Force General Hospital |
Kuo W.-W.,China Medical University at Taichung |
Lin Y.-M.,Changhua Christian Hospital |
And 7 more authors.
Pharmaceutical Biology | Year: 2013
Context: Statistical and clinical reports indicate that betel nut chewing is strongly associated with progression of oral cancer because some ingredients in betel nuts are potential cancer promoters, especially arecoline. Early diagnosis for cancer biomarkers is the best strategy for prevention of cancer progression. Several methods are suggested for investigating cancer biomarkers. Among these methods, gel-based proteomics approach is the most powerful and recommended tool for investigating biomarkers due to its high-throughput. However, this proteomics approach is not suitable for screening biomarkers with molecular weight under 10 KDa because of the characteristics of gel electrophoresis. Objective: This study investigated biomarkers with molecular weight under 10 KDa in rats with arecoline challenge. Materials and methods: The centrifuging vials with membrane (10 KDa molecular weight cut-off) played a crucial role in this study. After centrifuging, the filtrate (containing compounds with molecular weight under 10 KDa) was collected and spotted on a sample plate for MALDI-TOF mass spectrometry analysis. Results: Compared to control, three extra peaks (m/z values were 1553.1611, 1668.2097 and 1740.1832, respectively) were found in sera and two extra peaks were found in heart tissue samples (408.9719 and 524.9961, respectively). These small compounds should play important roles and may be potential biomarker candidates in rats with arecoline. Discussion and conclusions: This study successfully reports a mass-based method for investigating biomarker candidates with small molecular weight in different types of sample (including serum and tissue). In addition, this reported method is more time-efficient (1 working day) than gel-based proteomics approach (5~7 working days). © 2013 Informa Healthcare USA, Inc.
Fan Y.-G.,Taichung Armed Force General Hospital |
Hu C.-W.,National Cheng Kung University |
Chu C.,National Chiayi University |
Chiu K.-C.,National Chiayi University |
Weng B.B.C.,National Chiayi University
Food Research International | Year: 2012
Heavily evidenced in antimicrobial effects of microbial β-(1,3)(1,6)-glucan, the botanical β-(1,3)(1,4)-glucan is mostly shown to effectively control blood cholesterol level. S. Typhimurium belongs to food-borne zoonoses often causing worldwide epidemic outbreaks in animals and human with severe diarrhea and gastrointestinalitis. Cereal soups are commonly prescribed as neutraceutical for rehydration purpose and a sustaining therapy. Botanical β-(1,3)(1,4)-glucan is easily released in boiled cereal soup β-(1,3)(1,4)-Glucans on modulating host defense to enteric infectious agents is seldom reported. Our results demonstrated that barley β-(1,3)(1,4)-glucans effectively increased the murine macrophage cell line RAW264.7 against S. Typhimurium infection through antibacterial lysozyme activity (P < 0.001), not the production of intracellular reactive oxygen species. Furthermore, barley β-(1,3)(1,4)-glucans upregulated the gene expressions of its receptor dectin-1. In conclusion, barley β-(1,3)(1,4)-glucan induces a mild immune response with increasing antibacterial lysozymes through up-regulating its receptors dectin-1 and lysozyme M gene expressions. © 2011 Elsevier Ltd.
Chang-Lee S.N.,Asia University, Taiwan |
Hsu H.-H.,Mackay Memorial Hospital |
Hsu H.-H.,Nursing and Management College |
Shibu M.A.,China Medical University at Taichung |
And 9 more authors.
Pathology and Oncology Research | Year: 2016
Peroxisome proliferator-activated receptor-α (PPARα) is a member of the nuclear receptor superfamily involved in hepatocarcinogenesis in rodents. In previous studies on liver tumor tissues, PPARα mRNA expression was found to be significantly higher and overexpression of ERα inhibited the PPARα expression, cell-proliferation and also induced apoptosis in Hep3B cell. However, the role of ERβ is not known yet. Therefore, the aim of this study is to define the role of ERβ on PPARα in Hep3B cells. The effect of PPARα signaling cascade were monitored by inducing Hep3B cells by fenofibrate. Further the cells were transfected with pCMV-ERβ and the consequences of ERβ-overexpression on the PPARα induced changes such as enhanced cell-proliferation and suppressed apoptosis were determined using western blot analysis and TUNEL assay. The EMSA was used to identify whether ERβ modulates PPARα expression by binding to PPARα promoter region to repress PPARα promoter activity. In addition, the direct interaction between ERβ and PPARα proteins was verified by co-immunoprecipitation assay. Our results show that the overexpressed ERβ not only attenuated the effects of fenofibrate to induce the levels of apoptosis protein such as Cyt.c, Caspase 9 and Caspase 3 but also inhibited the levels of survival protein such Bcl-xL, p-Bad, cyclin A and cyclin E. All these effects of E2/ERβ resulted in the enhancement of mitochondria dependent apoptotic pathway and the attenuation of cell proliferation. Moreover, the overexpressed ERβ reduced the mRNA and protein levels of PPARα and its downstream Acyl-CoA oxidase (ACO). EMSA results show that ERβ directly binds to PPRE and inhibit PPARα gene expression and according to immunoprecipitation assay ERβ also binds strongly with PPARα. The E2/ERβ further inhibited the fenofibrate-induced nuclear translocation of PPARα. Taken together, ERβ might directly downregulate PPARα gene expression and inhibit the nuclear translocation to suppress the proliferation and induce the apoptosis of Hep3B cells. © 2016 Arányi Lajos Foundation
Tsai H.-T.,Taipei Medical University |
Tsai H.-T.,Chung Shan Medical University |
Hsieh M.-J.,Changhua Christian Hospital |
Hsieh M.-J.,Chung Shan Medical University |
And 12 more authors.
Tumor Biology | Year: 2014
The purpose of this study was to investigate genetic impact of TIMP-3 -1296 T>C (rs9619311) and TIMP-4 -55 T>C (rs3755724) gene polymorphisms on the susceptibility and clinicopathological characteristics of hepatocellular carcinoma (HCC). A total of 759 subjects, including 530 healthy controls and 229 patients with hepatocellular carcinoma, were recruited in this study. Allelic discrimination of TIMP-3 -1296 T>C (rs9619311) and TIMP-4 -55 T>C (rs3755724) polymorphisms was assessed with the ABI StepOne™ Real-Time PCR System. Among women group, individuals with TC or CC alleles of TIMP-3 -1296 T>C gene polymorphism protected against HCC (AOR = 0.35, 95% confidence interval (CI) = 0.12–0.97; p = 0.04) compared to individuals with TT alleles, after adjusting for other confounders. Also, women with TC alleles and with TC or CC alleles of TIMP-4 -55 T>C polymorphisms had a 2.52-fold risk (95%CI = 1.23–5.13; p = 0.01) and 2.47-fold risk (95%CI = 1.26–4.87; p = 0.008) of developing HCC compared to individuals with TT alleles, after adjusting for other confounders. There was no synergistic effect between gene polymorphism and environmental risk factors, including tobacco and alcohol consumptions and clinical statuses of HCC as well as serum expression of liver-related clinicopathological markers. In conclusion, gene polymorphisms of TIMP-3 -1296 T>C (rs9619311) and TIMP-4 -55 T>C (rs3755724) play a role in the susceptibility of HCC among Taiwan women. © 2014, International Society of Oncology and BioMarkers (ISOBM).
Chu W.-M.,Chung Shan Medical University |
Chu W.-M.,Taichung Veterans General Hospital |
Liao W.-C.,Chung Shan Medical University |
Liao W.-C.,National Defense Medical Center |
And 12 more authors.
Archives of Gerontology and Geriatrics | Year: 2016
Objective: To evaluate whether late-career unemployment is associated with increased all-cause mortality, functional disability, and depression among older adults in Taiwan. Method: In this long-term prospective cohort study, data were retrieved from the Taiwan Longitudinal Study on Aging. This study was conducted from 1996 to 2007. The complete data from 716 men and 327 women aged 50-64 years were retrieved. Participants were categorized as normally employed or unemployed depending on their employment status in 1996. The cumulative number of unemployment after age 50 was also calculated. Logistic regression analysis was used to examine the effect of the association between late-career unemployment and cumulative number of late-career unemployment on all-cause mortality, functional disability, and depression in 2007. Results: The average age of the participants in 1996 was 56.3 years [interquartile range (IQR) = 7.0]. A total of 871 participants were in the normally employed group, and 172 participants were in the unemployed group. After adjustment of gender, age, level of education, income, self-rated health and major comorbidities, late-career unemployment was associated with increased all-cause mortality [Odds ratio (OR) = 2.79; 95% confidence interval (CI) = 1.74-4.47] and functional disability [OR = 2.33; 95% CI = 1.54-3.55]. The cumulative number of late-career unemployment was also associated with increased all-cause mortality [OR = 1.91; 95% CI = 1.35-2.70] and functional disability [OR = 2.35; 95% CI = 1.55-3.55]. Conclusion: Late-career unemployment and cumulative number of late-career unemployment are associated with increased all-cause mortality and functional disability. Older adults should be encouraged to maintain normal employment during the later stage of their career before retirement. Employers should routinely examine the fitness for work of older employees to prevent future unemployment. © 2016.
Yang C.-C.,National Chung Hsing University |
Lin C.-C.,National Chung Hsing University |
Lin C.-C.,Taichung Armed Force General Hospital |
Liao J.-W.,National Chung Hsing University |
Yen S.-K.,National Chung Hsing University
Materials Science and Engineering C | Year: 2013
Through the hydrogen bonds and the deprotonation, the vancomycin-chitosan composite has been originally deposited on Ti4Al4V by electrochemical technology. However, the rapid destruction of the hydrogen bonding between them by polar water molecules during immersion tests revealed 80% drug burst in a few hours. In this study, the post porous hydroxyapatite (HA) coated Ti4Al4V is prepared for the subsequent electrolytic deposition of vancomycin-chitosan composite to control the drug release. As expected, the initial burst is reduced to 55%, followed by a steady release about 20% from day 1 to day 5 and a slower release of the retained 25% after day 6, resulting in bacterial inhibition zone diameter of 30 mm which can last for more than a month in antibacterial tests, compared with the coated specimen without HA gradually loosing inhibition zone after 21 days. Besides, the cell culture indicates that the vancomycin-chitosan/ HA composite coated has enhanced the proliferation, the differentiation and the mineralization of the osteoblast-like cell. In general, it is helpful for the osteointegration on permanent implants. Consistently, it effectively provides the prophylaxis and therapy of osteomyelitis according to the results of the rabbit infection animal model. © 2013 Elsevier B.V.
Hsu W.-T.,Taichung Armed Force General Hospital |
Pien H.-C.,Taichung Armed Force General Hospital |
Huang H.-I.,Central Taiwan University of Science and Technology |
Tu Y.-Y.,Taichung Armed Force General Hospital |
And 3 more authors.
Biomarkers and Genomic Medicine | Year: 2013
Atherosclerosis is a chronic inflammatory vascular disease, and an association between elevated blood lipids and atherosclerosis has previously been recognized. Low-density lipoprotein cholesterol (LDLc) levels have also been recognized as indicators of cardiovascular complications, but two people who have exactly the same LDLc concentrations may have different risk factors, and the National Cholesterol Education Program Adult Treatment Panel III (NCEP ATP III) guidelines recommend patching non-high-density lipoprotein cholesterol (non-HDLc) as another indicator. The present study investigated diabetic dyslipidemia to determine if there is a correlation between dyslipidemia and C-reactive protein (CRP). The data shows that non-HDLc was an independent risk factor for cardiovascular events in patients with diabetes and that CRP could indicate the risk of future cardiovascular events in both high-risk and healthy individuals. © 2013.
PubMed | China Medical University at Taichung, Chung Shan Medical University, Taichung Armed Force General Hospital and Tsaotun Psychiatric Center
Type: Journal Article | Journal: Biomedical reports | Year: 2015
The present study evaluated the effects of physiological serum estrogen during the menstrual cycle on glutathione (GSH) and catalase activities. The sample included 43 healthy females between the ages of 22 and 51 years. The subjects were divided into two groups according to the stage of the menstrual cycle. Group A consisted of 16 samples extracted between days 10 and 20 from the first day of menstruation when estrogen levels were considered to be at their highest. Group B consisted of 27 samples extracted during other times of the estimated 30 days of menstruation. Data showed that the estrogen level in group A (184106 pg/ml) was higher than that in group B (10556 pg/ml) (P<0.01). The GSH and catalase levels in group A (4.42.3 g/mg and 21072 IU/mg, respectively) were also significantly higher compared to the levels in group B (3.21.8 g/mg and 16862 IU/mg, respectively) (P <0.05). Spearmans rank correlation showed that the expression of catalase in red blood cells significantly correlated with serum estrogen level but not with GSH. However, the changes in estrogen plasma levels, erythrocyte GSH level and catalase activity suggested that the consumption of GSH and catalase in erythrocyte during the menstrual cycle may be associated with the level of estrogen present in the bloodstream.