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Anusha K.,HIGH-TECH | Balakrishnan S.,HIGH-TECH | Sindhu S.,Armats Biotek Training and Research Institute | Hariram S.B.,HIGH-TECH
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2014

Synthetic chelators strongly bind to metal ions which used for iron excretion by binding to ions to produce metal chelator complex for the removal of metals from the body .Metals with normal concentration have essential roles in body metabolism however; in higher concentration they can induce sever toxicity. Treatment with chelating agent is an optimal method to reduce metals toxicity in organisms .Hence the present study was undertaken to evaluate the chelating property of Trichodesma indicum a common weed of India.. The chelating effect of ferrous ions by the extracts was estimated by the method of Dinis et.in various concentrations of the extract of the selected plant by adding 0.05ml of 2mM FeCl and initiated Ferrozine(5mM). Absorbance of the solution was then measured spectrophotometrically at 562nm.The Metal Chelating Activity indicated that Trichodesma indicum has the ability for iron binding and could reduce the generation of hydroxyl radicals. From the result, it is clear that ethyl acetate extract of Trichodesma indicum has significant metal chelating activity. It is reported that chelating agents are effective as secondary antioxidants because they reduce the redox potential, thereby stabilizing the oxidized form of the metal ion. Since results are quite promising the present work can be further extended to screen toxicology to ascertain its safe administration as drug through animal studies. Source

Sridhar S.,Bharathiar University | Chinnathambi V.,Armats Biotek Training and Research Institute | Arumugam P.,Armats Biotek Training and Research Institute | Suresh P.K.,Vellore Institute of Technology
Applied Biochemistry and Biotechnology | Year: 2013

The objective of this paper is to compare in silico data with wet lab physicochemical properties of crude laccase enzyme isolated from Rigidoporus sp. using wheat bran as solid substrate support towards dye decolorization. Molecular docking analysis of selected nine textile and non-textile dyes were performed using laccase from Rigidoporus lignosus as reference protein. Enzyme-based remediation methodology using crude enzyme enriched from solid state fermentation was applied to screen the effect of four influencing variables such as pH, temperature, dye concentration, and incubation time toward dye decolorization. The extracellular crude enzyme decolorized 69.8 % Acid Blue 113, 45.07 % Reactive Blue 19, 36.61 % Reactive Orange 122, 30.55 % Acid Red 88, 24.59 % Direct Blue 14, 18.48 % Reactive Black B, 16.49 % Reactive Blue RGB, and 11.66 % Acid Blue 9 at 100 mg/l dye concentration at their optimal pH at room temperature under static and dark conditions after 1 h of incubation without addition of any externally added mediators. Our wet lab studies approach, barring other factors, validate in silico for screening and ranking textile dyes based on their proximity to the T1 site. We are reporting for the first time a combinatorial approach involving in silico methods and wet lab-based crude laccase-mediated dye decolorization without any external mediators. © Springer Science+Business Media New York 2013. Source

Ezhilarasan V.,Armats Biotek Training and Research Institute | Chinnathambi V.,Armats Biotek Training and Research Institute | Janarthanan V.,Presidency College at Chennai | Yazhini K.A.,Kerala University | Sridhar S.,Jeppiaar Engineering College
International Journal of Pharma and Bio Sciences | Year: 2012

Cyclooxygenase-2 is being treated as one of the chief anti-cancer targets for colorectal, lung, breast, prostate and head/neck cancer. The focus of this study is to discover new ligand molecules, which can be used as a potential drug against Cyclooxygenase-2. The structure of Cyclooxygenase-2 of Homo sapiens was modeled using "MODELLER". The FDA approved and experimental level drugs are available in DrugBank3.0 database was screened against Cyclooxygenase-2 using the virtual screening facility offered by PYR-X0.8 software. Molecular docking studies were performed using AutoDock Wizard and the results were analyzed critically with the help of AutoDock tools 1.4.5. Virtual Screening and Molecular Docking Analysis revealed four molecules. Namely, N-cyclopropyl-4-methyl-3-[1-(2-methylphenyl) phthalazin-6-yl]benzamide, 6-Fluoro-2-(2'-Fluoro-1,1'-Biphenyl-4-Yl)-3-Methylquinoline-4-Carboxylic Acid, Eletriptan and Tamibarotene. Source

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