Armats Biotek Pvt Ltd

Guindy, India

Armats Biotek Pvt Ltd

Guindy, India
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Rajesh V.,Government Arts College Autonomous Nandanam | Sophiya J.,St. Joseph's College | Justin Packia Jacob S.,St. Joseph's College | Arumugam P.,Armats Biotek Pvt. Ltd. | Jayaraman P.,Government Arts College Autonomous Nandanam
Journal of Bionanoscience | Year: 2017

The aim of the present study is to evaluate the antioxidant, antimicrobial, anti-inflammatory and anticancer activities of silver nanoparticles (AgNP) synthesized ("green synthesis") using the Diospyros ferrea leaves of the family Ebenaceae, collected from Damanur village hills, Gingee taluk, Villupuram district, Tamil Nadu. The freshly cleaned leaves of the plants were used for the synthesis of silver nanoparticles by varying the time of exposure of the reaction mixture to sunlight, UV and boiling temperature. The structural details of the synthesized silver nanoparticles were elucidated using X-ray diffraction method (XRD), Fourier Transform Infra-Red Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) and EDAX. The antioxidant and antimicrobial potential of the silver nanoparticles was effective in which the IC50 was determined to be 227.58 μg/mL for free radical scavenging activity. The antimicrobial activity was performed by Agar well diffusion assay using Staphylococcus aureus, Micrococcus luteus, Escherichia coli, Klebsiella pneumoniae Aspergillus flavus and Aspergillus fumigatus. The anti-inflammatory potential of the nanoparticles was studied by albumin denaturation assay in which maximum inhibition was found to be 65.21% at a concentration of 100 μg/mL and its IC50 was determined to be 76.67 μg/ml. Anticancer activity of the plant using MCF-7 and HepG2 cell lines shows its IC50 Value determined to be 71.41 and 59.59 μg/mL respectively. The result of the studies showed the silver nanoparticles synthesized using D. ferrea leaf extract was found to have significant antioxidant, antimicrobial and anti-inflammatory properties along with definitive anti-proliferative activity.


Samuel Akash Raj R.,St. Joseph's College | Divya S.,St. Joseph's College | Sindhu S.,Armats Biotek Pvt Ltd. | Kasinathan K.,University of Madras | Arumugam P.,Armats Biotek Pvt Ltd.
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2014

Objective: The present study is to synthesize nanoparticles using the leaf extracts of Mimosa pudica and screen for its α-amylase inhibiting potential as well as antibacterial properties. Methods: Silver nanoparticles were synthesized using extract of Mimosa pudica leaves under various conditions such as sunlight, UV and room temperature. The synthesized nanoparticles were further characterized using UV, FTIR, XRD and SEM analyses. The inhibitory effect of the synthesized nanoparticles on bacterial pathogens and α-amylase activity was also studied. Results: Exposure of reaction mixture to sunlight produced maximum amount of nanoparticles, comparatively. The particles showed a dose-dependent increase in percentage inhibitory activity on α-amylase enzyme. Moreover, the nanoparticles showed significant inhibitory effect on Bacillus subtilis and Klebsiella pneumonia. Conclusion: Thus the silver nanoparticles synthesized using M. pudica leaves prove to be a significant source of therapeutic agents, however further mechanistic studies are necessary.


Radhika S.,Bharathiar University | Senthil K.R.,Government of Tamilnadu | Sindhu S.,Armats Biotek Pvt Ltd | Sagadevan E.,Aachi Masala Foods P Ltd | Arumugam P.,Armats Biotek Pvt Ltd
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2013

Objective: The current study is aimed at evaluating the antidiabetic efficacy of Premna corymbosa and to evaluate its phytochemical constituents. Methods: The leaves of P. corymbosa were subjected to extraction with methanol, ethyl acetate and hexane. The extracts were scrutinized for their antidiabetic potential using α amylase inhibitory activity, non-enzymatic glycosylation of hemoglobin, glucose uptake by yeast cells and glucose diffusion assays. Besides the plant extract was also subjected to qualitative and quantitative phytochemical studies followed by thin layer chromatography. Results: The results of α- amylase inhibition assay revealed that the methanol extract was significant with IC50 of 145.72μg/ml, when compared with ethyl acetate and hexane extracts. The inhibition of glycosylation of hemoglobin was in a dose dependent manner, conversely the glucose transport and diffusion differs with the sample and glucose concentration. Qualitative phytochemical analysis of the methanol extract confirms the presence of various phytochemicals like flavonoids, phenol, tannin, alkaloids, carbohydrates, terpenoids, coumarins, quinones and cardiac glycosides. Furthermore, phenols, flavonoids and tannins were quantified to be 734.54 GAE/g, 738.95 QE/g and 224.24 GAE/g, respectively. Conclusion: The results of the work consequently signify the potential of the extract to manage hyperglycemia. However, these effects need to be confirmed using in vivo models and clinical trials for its effective utilization as therapeutic agents.


Suhashini R.,Sree Sastha Institute of Engineering and Technology | Sindhu S.,Armats Biotek Pvt. Ltd | Sagadevan E.,Aachi Masala Foods Pvt Ltd
International Journal of Pharmacognosy and Phytochemical Research | Year: 2014

Diabetes is a metabolic disorder that occurs due to poor secretion of insulin by pancreas. Insulin helps to metabolize blood sugar. Hence this results in high glucose level in blood. Presently, diabetes is considered as a big killer and is among the most significant diseases in the developed world. The incidence of diabetes is increasing every day and this indicates the increasing need for the treatment of diabetes. The blood glucose level can be regulated by various mechanisms. In vitro antidiabetic assays such as glucose uptake by yeast cells, glucose diffusion assay, alpha amylase inhibition assay and glycosylation of hemoglobin assay are performed. Using these assays, the antidiabetic activity of the plant Psoralea corylifolia is determined. Qualitative and quantitative phytochemical analysis reveals all the bioactive compounds present in the crude extract of the plant. Our results show that methanolic extract has an effective activity when compared to ethyl acetate and hexane extracts. Hence methanolic extract of Psoralea corylifolia possess higher antidiabetic activity. The compounds which contribute to its antidiabetic activity were identified, characterized and separated by thin layer chromatography. MTT assay for L6 cell lines was performed and was found that the plant extract showed a minimum toxicity.


Rajesh V.,Government Arts College for Men Autonomous | Arumugam P.,Armats Biotek Pvt. Ltd | Jayaraman P.,Government Arts College for Men Autonomous
International Journal of Pharmacognosy and Phytochemical Research | Year: 2015

Dried Leaves of Pavonia procumbens (wall. ex wight & Arn.) walp. were collected from Damanur hills, Villupuram District, Tamil Nadu, India for the comparative analysis of phyto-chemical potential with reference to antioxidant and antidiabetic activity. The active plant compounds were extracted with ethyl acetate, hexane and methanol solvents. Among the solvents used for extraction, methanol found to be a suitable solvent and hence used for further extraction and analysis of biocompounds. The preliminary qualitative phyto-chemical screening of Pavonia procumbens has revealed the presence of Phenols, Flavanoids and Terbenoids and absence of Alkaloids, Tannins and Reducing sugars in methanol extract. The quantitative determination of phenol and flavanoids were carried out and found that the total phenolic content with 1. 6095μg GAE/ml and flavonoids 2. 071μg QE/ml respectively. The IC50 value of DPPH assy, Nitric oxide scavenging assay and Fe2+ Chelation assay of methanol extract were recorded as 200. 41 μg/ml, 108. 81 μg/ml and 386. 72 μg/ml respectively. The Phosphomolybdenum assay and reducing power assay indicate that the plant extract is a potential antioxidant. The results for the antidiabetic assay by α-amylase inhibition exhibited from 27%-79% in the concentration of 5-25 μg/ml and non enzymatic glycosylation of hemoglobin shows 33%-73% in the concentration of 250-1000 μg/ml. The inhibitory activity of Glucose uptake by yeast cells in methanol extract of Pavonia procumbens were found and compared with the standard drug acarbose. The details of the above results are discussed in the present study. © 2015, International Journal of Pharmacognosy and Phytochemical Research. All Rights Reserved.


Manonmani V.,Sathyabama University | Juliet V.,SRM University | Ponnidevi J.,Sathyabama University | Arumugam P.,Armats Biotek Pvt.
Advanced Materials Research | Year: 2014

Food safety has emerged as an important global issue. Illness from the pathogenic bacteria is a significant health concern. Pathogens are potentially harmful and it contaminate food and causes foodborne illness. It is very important for early detection of food borne pathogens. Conventional methods of detecting these pathogens eventhough sensitive, but still it is time consuming, complex and laborious. Biosensors have great potential for the detection of pathogenic bacteria in food. Biosensors are developed to rapidly detect the pathogens. In this paper, we use nano-sized magnetite nanoparticles coated with chitosan, a polymer to capture and identify bacteria from contaminated food sample and eliminates the purpose of specific antibody coating. Amine group with magnetite nanoparticles(MNPs) specifically binds with the bacterial cell wall, on their surface of most pathogenic bacteria present in food. Rhodamine as a dye/marker which emits fluorescence in exposure to light used to detect the bacterial concentration in terms of light or fluorescent intensity and analog voltage using Magneto-Fluorescent biosensor. The developed biosensor could be able to detect bacteria in the limit of 10 CFU/ml and the time taken for measurement of a sample using biosensor would be less than 5 minutes. Hence the developed biosensor are non-specific and highly sensitive. © (2014) Trans Tech Publications, Switzerland.


Sathak Sameer S.M.,HIGH-TECH | Manigandan P.,HIGH-TECH | Sekar Babu H.,HIGH-TECH | Sivaraj C.,Armats Biotek Pvt Ltd | And 2 more authors.
International Journal of Pharma and Bio Sciences | Year: 2014

The current study was aimed at evaluation of Antioxidant & Anti proliferative potential of the methanol extract of Heracleum sprengelianum leaves and to elucidate its phytochemical profile using GC-MS analysis. The methanol extract of leaves of H. sprengelianum as screened for its antioxidant potential by dot-plot assay, DPPH radical scavenging assay and hydroxyl radical scavenging assay. Since the results were quite encouraging, it stimulated the investigators to proceed further to screen the phytochemical profiles by qualitative and quantitative methods which were further extended to thin layer chromatography, GC-MS analysis and antiproliferative activity. The result indicates that the methanol extract of leaves of H. sprengelianum showed significant radical scavenging activity, potent toxicity to liver cancer cells and also less toxicity to normal cells. The GC-MS result showed the presence of important active metabolites such as fatty acids and phenolic acids. Since the results are promising, H. sprengelianum could be considered as a significant source of Antioxidant and Anti proliferative agents.


Giridharan T.,HIGH-TECH | Chandran M.,HIGH-TECH | Sindhu S.,Armats Biotek Pvt Ltd | Arumugam P.,Armats Biotek Pvt Ltd
International Journal of Pharma and Bio Sciences | Year: 2014

The aim of the study is to evaluate the therapeutic applications of silver nanoparticles synthesized using 2 medicinal plants collected from yelagiri hills. The leaves of the plants were used for optimization of silver nanoparticles by varying the time exposure of the reaction mixture to sunlight (5, 10, 15 minutes). The anti-oxidant, anti inflammatory and antimicrobial potentials of samples was studied by different assays. Also, the synthesized nanoparticles were characterized by UV, SEM, XRD and FTIR techniques. The results suggest that nanoparticles synthesis was significant at exposure time of 5 and 10minutes. The synthesized particles were confirmed by UV spectroscopy, which showed a characteristic peak at 427 and 418nm for the 2 samples respectively. The synthesized nanoparticles were found to be in the size range of 60-80nm and possessed characteristic XRD peaks. The results of the study revealed that the synthesized silver nanoparticles possessed significant antioxidant, anti inflammatory, anti-proliferative and antimicrobial properties.


Giridharan T.,HIGH-TECH | Masi C.,HIGH-TECH | Sindhu S.,Armats Biotek Pvt Ltd | Arumugam P.,Armats Biotek Pvt Ltd
Biosciences Biotechnology Research Asia | Year: 2014

The aim of this study is to evaluate the anti-proliferative activity of silver nanoparticles synthesized using D.viscosa and C.decidua. The leaves of the plants were used for optimization of silver nanoparticles by varying the time exposure of the reaction mixture to sunlight (5,10, 15 minutes). The anti-oxidant potential of samples was studied by different assays. Also, the synthesized nanoparticles were characterized by UV, SEM, XRD and FTIR techniques. The results suggest that nanoparticles synthesis was significant at exposure time of 5 and 10 minutes. The synthesized particles were confirmed by UV spectroscopy which showed characteristic peak at 421nm for both the samples. The synthesized nanoparticles were found to be in the size range of 60-90nm and possessed characteristic XRD peaks. The results of the study revealed that the synthesized silver nanoparticles possessed significant antioxidant, anti inflammatory, anti-proliferative and antimicrobial properties.

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