Guindy, India
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Rajesh V.,Government Arts College Autonomous Nandanam | Sophiya J.,St. Joseph's College | Justin Packia Jacob S.,St. Joseph's College | Arumugam P.,Armats Biotek Pvt. Ltd. | Jayaraman P.,Government Arts College Autonomous Nandanam
Journal of Bionanoscience | Year: 2017

The aim of the present study is to evaluate the antioxidant, antimicrobial, anti-inflammatory and anticancer activities of silver nanoparticles (AgNP) synthesized ("green synthesis") using the Diospyros ferrea leaves of the family Ebenaceae, collected from Damanur village hills, Gingee taluk, Villupuram district, Tamil Nadu. The freshly cleaned leaves of the plants were used for the synthesis of silver nanoparticles by varying the time of exposure of the reaction mixture to sunlight, UV and boiling temperature. The structural details of the synthesized silver nanoparticles were elucidated using X-ray diffraction method (XRD), Fourier Transform Infra-Red Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM) and EDAX. The antioxidant and antimicrobial potential of the silver nanoparticles was effective in which the IC50 was determined to be 227.58 μg/mL for free radical scavenging activity. The antimicrobial activity was performed by Agar well diffusion assay using Staphylococcus aureus, Micrococcus luteus, Escherichia coli, Klebsiella pneumoniae Aspergillus flavus and Aspergillus fumigatus. The anti-inflammatory potential of the nanoparticles was studied by albumin denaturation assay in which maximum inhibition was found to be 65.21% at a concentration of 100 μg/mL and its IC50 was determined to be 76.67 μg/ml. Anticancer activity of the plant using MCF-7 and HepG2 cell lines shows its IC50 Value determined to be 71.41 and 59.59 μg/mL respectively. The result of the studies showed the silver nanoparticles synthesized using D. ferrea leaf extract was found to have significant antioxidant, antimicrobial and anti-inflammatory properties along with definitive anti-proliferative activity.


Manonmani V.,Sathyabama University | Juliet V.,SRM University | Ponnidevi J.,Sathyabama University | Arumugam P.,Armats Biotek Pvt.
Advanced Materials Research | Year: 2014

Food safety has emerged as an important global issue. Illness from the pathogenic bacteria is a significant health concern. Pathogens are potentially harmful and it contaminate food and causes foodborne illness. It is very important for early detection of food borne pathogens. Conventional methods of detecting these pathogens eventhough sensitive, but still it is time consuming, complex and laborious. Biosensors have great potential for the detection of pathogenic bacteria in food. Biosensors are developed to rapidly detect the pathogens. In this paper, we use nano-sized magnetite nanoparticles coated with chitosan, a polymer to capture and identify bacteria from contaminated food sample and eliminates the purpose of specific antibody coating. Amine group with magnetite nanoparticles(MNPs) specifically binds with the bacterial cell wall, on their surface of most pathogenic bacteria present in food. Rhodamine as a dye/marker which emits fluorescence in exposure to light used to detect the bacterial concentration in terms of light or fluorescent intensity and analog voltage using Magneto-Fluorescent biosensor. The developed biosensor could be able to detect bacteria in the limit of 10 CFU/ml and the time taken for measurement of a sample using biosensor would be less than 5 minutes. Hence the developed biosensor are non-specific and highly sensitive. © (2014) Trans Tech Publications, Switzerland.


Sathak Sameer S.M.,HIGH-TECH | Manigandan P.,HIGH-TECH | Sekar Babu H.,HIGH-TECH | Sivaraj C.,Armats Biotek Pvt Ltd | And 2 more authors.
International Journal of Pharma and Bio Sciences | Year: 2014

The current study was aimed at evaluation of Antioxidant & Anti proliferative potential of the methanol extract of Heracleum sprengelianum leaves and to elucidate its phytochemical profile using GC-MS analysis. The methanol extract of leaves of H. sprengelianum as screened for its antioxidant potential by dot-plot assay, DPPH radical scavenging assay and hydroxyl radical scavenging assay. Since the results were quite encouraging, it stimulated the investigators to proceed further to screen the phytochemical profiles by qualitative and quantitative methods which were further extended to thin layer chromatography, GC-MS analysis and antiproliferative activity. The result indicates that the methanol extract of leaves of H. sprengelianum showed significant radical scavenging activity, potent toxicity to liver cancer cells and also less toxicity to normal cells. The GC-MS result showed the presence of important active metabolites such as fatty acids and phenolic acids. Since the results are promising, H. sprengelianum could be considered as a significant source of Antioxidant and Anti proliferative agents.


Giridharan T.,HIGH-TECH | Chandran M.,HIGH-TECH | Sindhu S.,Armats Biotek Pvt Ltd | Arumugam P.,Armats Biotek Pvt Ltd
International Journal of Pharma and Bio Sciences | Year: 2014

The aim of the study is to evaluate the therapeutic applications of silver nanoparticles synthesized using 2 medicinal plants collected from yelagiri hills. The leaves of the plants were used for optimization of silver nanoparticles by varying the time exposure of the reaction mixture to sunlight (5, 10, 15 minutes). The anti-oxidant, anti inflammatory and antimicrobial potentials of samples was studied by different assays. Also, the synthesized nanoparticles were characterized by UV, SEM, XRD and FTIR techniques. The results suggest that nanoparticles synthesis was significant at exposure time of 5 and 10minutes. The synthesized particles were confirmed by UV spectroscopy, which showed a characteristic peak at 427 and 418nm for the 2 samples respectively. The synthesized nanoparticles were found to be in the size range of 60-80nm and possessed characteristic XRD peaks. The results of the study revealed that the synthesized silver nanoparticles possessed significant antioxidant, anti inflammatory, anti-proliferative and antimicrobial properties.


Giridharan T.,HIGH-TECH | Masi C.,HIGH-TECH | Sindhu S.,Armats Biotek Pvt Ltd | Arumugam P.,Armats Biotek Pvt Ltd
Biosciences Biotechnology Research Asia | Year: 2014

The aim of this study is to evaluate the anti-proliferative activity of silver nanoparticles synthesized using D.viscosa and C.decidua. The leaves of the plants were used for optimization of silver nanoparticles by varying the time exposure of the reaction mixture to sunlight (5,10, 15 minutes). The anti-oxidant potential of samples was studied by different assays. Also, the synthesized nanoparticles were characterized by UV, SEM, XRD and FTIR techniques. The results suggest that nanoparticles synthesis was significant at exposure time of 5 and 10 minutes. The synthesized particles were confirmed by UV spectroscopy which showed characteristic peak at 421nm for both the samples. The synthesized nanoparticles were found to be in the size range of 60-90nm and possessed characteristic XRD peaks. The results of the study revealed that the synthesized silver nanoparticles possessed significant antioxidant, anti inflammatory, anti-proliferative and antimicrobial properties.

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