Arid Zone Research Center

Quetta, Pakistan

Arid Zone Research Center

Quetta, Pakistan
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Razzaq A.,Arid Zone Research Center | Ashraf K.,The University of Lahore | Maqbool A.,The University of Lahore | Khan M.A.,The University of Lahore | Islam M.,Pakistan Agricultural Resaerch Council
Journal of Animal and Plant Sciences | Year: 2013

In present research study, three experiments were launched during January 2011 to December 2011. Experiment I, Epidemiology: A total 1200 faecal samples (100 per month) were collected from farmers Bibrik-sheep (either sexes, 1-5 years old) and analyzed for nematodes prevalence. Experiment II, Sero-diagnosis: The sera samples of healthy and nematodes infected sheep were analyzed through ELISA. Experiment III, Therapeutic trial: comparative efficacy of herbal and synthetic anthelmintics was determined. Results showed that overall 40.25% Bibrik-sheep were infected with nematodes. Five nematode genera were recorded with higher prevalence of Haemonchus (10.42%) followed by Strongyloides (8.75%), Trichostrongylus (7.33%), Nematodirus (6.92%), and Trichuris (6.83%). The older age ewes presented higher nematodes prevalence. These five genera of nematodes were prevalent throughout the year. However, two peaks of nematodes prevalence i.e. March/May and August/December 2011 were observed. The level of nematodes infection was mostly low (< 2000 EPG) and did not impair the sheep productivity seriously. Haemonchus and Trichuris positive samples, based on coprological examination indicated 100% positive sensitivity for each genus of nematode by the ELISA, based on crude somatic antigen, while based on excretory antigen, showed lower (92%) sensitivity. The higher (86-100%) reduction of EPG was recorded in sheep treated with Ivermectin, while lower (68-96%) with Atreefal deedan.

Hamdullah A.,Brewery Road | Lateef M.,University of Veterinary and Animal Sciences | Maqbool A.,University of Veterinary and Animal Sciences | Jabbar M.A.,University of Veterinary and Animal Sciences | And 4 more authors.
Pakistan Journal of Zoology | Year: 2015

In present study, four sheep breeds (i.e., Balochi, Rakhshani, Karakul and Cross) were evaluated for resistance with three anthelmintics (Oxfendazole. Levamisole and Ivermectin) against nematodes (Haemonchus, Trichostrongylus, Nematodirus and Ostertagia) between April 2011 to March 2012 at Maslakh Sheep Farm, Pishin district, Balochistan. The overall results showed that there was reduction of EPG between 95% to 99% indicating the susceptibility of these anthelmintics against nematodes in the study area. The results of egg hatch test showed LC 50 less than 0.1μg/ml of oxfendazole. This indicated that no resistance was found in eggs hatching to oxfendazole. In addition, the Egg hatch test also confirmed the result of Faecal Egg Count Reduction Test. The qualitative examination of faecal samples after Coproculture revealed four gastrointestinal nematode genera, Haemonchus contortus, Trichostrongylus, Nematodirus and Ostertagia. Copyright 2015 Zoological Society of Pakistan.

Tehmina S.,University of Balochistan | Shahina R.,University of Balochistan | Razzaq A.,Arid Zone Research Center | Marghazani I.B.,University of Veterinary and Animal Sciences | Khosa A.N.,University of Veterinary and Animal Sciences
Revista Veterinaria | Year: 2014

The prevalence of Paramphistomum cervi infestation in Balochi, Babrik and Harnai sheep breeds from Balochistan (Pakistan) was studied during September to December 2010. For this purpose, rumen of slaughtered sheep in Quetta City and surroundings (n=1,200) were examined. Results showed significant differences (p<0.05) among age, sex, breed and season of P. cervi infestation in animals. The overall P. cervi infestation (17.83%) was recorded in animals during the study period. Considering age, a higher prevalence (p<0.05) of infestation (23.5%) was observed in animals older than two years-old. On the contrary, lower parasitation (p<0.05, 16.66%) was detected in less than one year-old group. Regarding sex, females (22.33%) were found to be more infested (p<0.05) than males (17.83%). The Balochi and Harnai sheeps showed higher prevalence of infestation (p<0.05) compared to Babrik sheeps. Considering the season of the year, P. cervi prevalence was the highest (p<0.05, 19.25%) during October, followed by November (15.75%), September (12.75%) and December (12.5%). © 2013.

Kakar H.,Spiny Road | Lateef M.,University of Veterinary and Animal Sciences | Maqbool A.,University of Veterinary and Animal Sciences | Jabbar M.A.,University of Veterinary and Animal Sciences | And 5 more authors.
Pakistan Journal of Zoology | Year: 2013

An epidemiological study was conducted to evaluate the prevalence and intensity of gastrointestinal nematodes of sheep in slaughter house at Quetta, Balochistan, from April 2011 to March 2012. A total of 960 gastrointestinal tract samples (80 per month) of sheep were examined during the study period. The overall prevalence of gastrointestinal nematode was 49.47% with the intensity of 516.63 in sheep. The higher monthly prevalence of gastrointestinal nematodes was observed during April (65%) followed by November (62.5%) and October (57.5%). Statistically there was significant differences (P<0.05) amongst months. The genus-wise intensity among gastrointestinal nematodes was presented higher by Haemonchus (213.05), followed by Trichostrongylus (137.05), Trichuris (90.1), Ostertagia (44.21) and Nematodirus (32.21). Breed-wise prevalence of gastrointestinal nematodes was higher in Balochi (50.20%) than the Harnai breed (48.75%). While, sex-wise prevalence of gastrointestinal nematodes was higher in female (56.87%) as compared to the male (42.08%). Age-wise prevalence of gastrointestinal nematodes in sheep was higher in the age group of < 1 year (60.83%) followed >2 years (43.33%) and 1-2 years (41.25%). Statistically there was no significant difference P > 0.05 between breeds, sex and age groups. Five genera of gastrointestinal nematode parasites were recovered during study period. Among these Haemonchus was the highest (71.36%) prevalent followed by Trichostrongylus (58.49%), Trichuris (28.84%), Ostertagia (17.26%) and Nematodirus (9.47%). © 2013 Zoological Society of Pakistan.

Bakhsh A.,Arid Zone Research Center | Iqbal S.M.,Pakistan Agricultural Research Council | Cheema N.M.,Pakistan Agricultural Research Council
Pakistan Journal of Botany | Year: 2013

Inheritance of testa colour, testa pattern, cotyledon color and stem color was studied in crosses of lentil involving parents with brown, grey, green testa color, red and yellow cotyledon color, and with green and colored seedling stem. Analysis of F1 plant populations of crosses between green and colored (pink) stem revealed that colored stem was dominant over green stem. Similarly red cotyledon was dominant over yellow cotyledon. The F2 segregation patterns indicated red cotyledon and colored stem to be monogenic traits. The analysis of F2 and F3 seeds (having F1 and F2 testa, respectively) revealed that spackled testa was dominant to even (without speckles) testa, grey testa was dominant to green testa and brown testa was dominant to both grey and green testa. The F2 segregation ratio from cross involving parents with brown and grey testa was 9 brown: 3 grey: 4 green. The crosses between parents with grey and green testa color segregated to fit a ratio of 3:1 between dominant and recessive color. These segregating patterns revealed involvement of two genes and a recessive inhibitor in the regulation of testa color.

Ahmad S.,Arid Zone Research Center | Gul S.,University of Balochistan | Achakzai A.K.K.,University of Balochistan | Islam M.,Arid Zone Research Center
Phyton | Year: 2010

Seedling growth and development of Seriphidium quettense were examined under water stress (WS) and non-water stress (NWS) conditions. Seedlings were grown in pots to investigate their response to NWS conditions. Impact of WS on seedling growth was assessed in pre-existing plants grown under a semi-arid, natural environment. Seedling growth attributes were recorded on a monthly basis. Seedling growth exhibited a significant positive response under NWS conditions. Length of the main root axis at the first leaf emergence stage was 5.69 cm under NWS conditions; however, it was 4.8 cm in the natural environment (WS). At the end of the growing season, length of their primary root was 118 cm in NWS conditions. At the same time, it reached 8.8 cm in the WS natural environment. Lateral roots emerged from 2.09 cm of the main root axis, and a high lateral root density was found in the 2-10 cm region. The average lateral root length of NWS plants by the end of the first growing season was 33.07 cm, whereas in the WS environment it was 21 cm. Shoot length was 0.34 and 27.4 cm at the beginning and end of the growing season, respectively, under NWS conditions. In the natural WS environment, shoot length was 0.78 and 21 cm at the beginning and end of the same growing season, respectively. Under NWS conditions, seedlings remained green all year round, and 41% of the seedlings reached their physiological maturity. Mean values for number of panicles/plant, panicle length, number of floral branches/panicle, length of floral branches, number of florets/capitulum, and number of seeds/capitulum were 3.6, 16.77 cm, 43.7, 5.46 cm, 3.89, and 1.12, respectively, on seedlings which reached reproductive maturity. However, seedlings didn't exhibit a similar response in the natural WS environment. Our results support that Seriphidium quettense has a substantial potential for establishment and population propagation if supplemental water is provided during their first year of growth.

Qasim Khan M.,Pakistan National Agricultural Research Center | Irshad H.,Pakistan National Agricultural Research Center | Jahangir M.,Pakistan National Agricultural Research Center | Razzaq A.,Arid Zone Research Center
OIE Revue Scientifique et Technique | Year: 2012

This paper presents data on the prevalence, biology and control of warble fly infestation (WFI) in cattle and goats in Pakistan. A questionnaire for obtaining information on biology and prevalence was circulated amongst field veterinary staff and livestock farmers in all five provinces (Punjab, Sindh, Balochistan, Khyber Pakhtunkhwa [KPK] and Gilgit-Baltistan) and in the Federally Administered Tribal Areas of the country. A total of 1,019 questionnaires were received (Punjab = 296, Sindh = 246, KPK = 318, Balochistan = 151, Gilgit-Baltistan = 8). Warble fly infestation was reported from each province and from the federally administered tribal areas, particularly from hilly, semi-hilly and sandy desert areas (the Cholistan desert, which adjoins the Rehim Yar Khan, Bahawalpur and Bahawalnagar districts, and the Nara area of Sanghar district). Warbles (nodules) started appearing on the backs of the infested animals from September through December and disappeared from October through March. The prevalence of WFI varied from 5% to 75%. It was highest in hilly areas and gradually decreased towards the plains. A map was developed of warble flyinfested areas. Four field trials were conducted to study the efficacy of different drugs indicated for the control of warble fly infestation. A total of 2,094 cattle and 3,876 goats were given five different injectables (avermectins); namely, Ivomec, Endectin, Euvectin, Dectomax and Promectin (ivermectin) during the first three weeks of September. A control group was given normal saline. All the medicines were found to be effective in controlling infestation.

Khan S.,Arid Zone Research Center | Khan J.,Arid Zone Research Center | Islam N.,Arid Zone Research Center | Islam M.,Arid Zone Research Center
Pakistan Journal of Botany | Year: 2011

Eleven wheat genotypes in Micro-plot Wheat Yield Trial including two checks (AZRI-96 and local) were evaluated for drought, yield and disease resistance in order to incorporate the high yielding and disease resistant genotypes in our wheat breeding program. The experiment was conducted at Arid Zone Research Centre, Quetta under rainfed conditions during 2006-07. Microplot genotypes No. 1, 8 and 7 performed tremendously well as compared with the local checks. They produced significantly higher yield of 1345, 1307 and 1246 kg/ha with good drought resistance and higher resistance to stripe rust while the local check produced grain yield 1119 kg/ha and susceptible to yellow rust.

Mansoor M.,Arid Zone Research Center | Islam Z.,Pakistan National Agricultural Research Center | Muhammad S.,Pakistan National Agricultural Research Center | Ali A.,Gomal University | Elahi M.E.,Arid Zone Research Center
Pakistan Journal of Botany | Year: 2016

To explore the effect of PGPR for improvement in grain yield of mungbean, different levels of fertilizer (00:00, 20:50 and 20:70 kg N:P2O5 kg ha-1) were tested on four strains (CB 1015 Australia, Vm-L1, Vm-M1 and Vm-M2) at Agricultural Research Institute, Dera Ismail Khan. A Randomized Complete Block Design with split plot arrangement was used to conduct the experiment by keeping three fertilizer levels in the main plot and Rhizobium strains in sub-plot. The data were recorded for different parameters like number of branches plant-1, number of clusters plant-1, number of pods plant-1., number of grain pod-1, pod length (cm) and grain yield kg ha-1. The response of fertilizer levels and inoculation with Rhizobium strains were found significant in most of the parameters under study. The strain Vm-M1 produced the highest number of branches plant-1 (5.42), number of clusters plant-1 (22.92), number of pods plant-1 (77.64), pod length (8.37 cm) when applied in combination of N:P2O5 @ 20:70 and 20:50 kg ha-1. Although the grain yield (1421 kg ha-1) recorded in treatment (20:70 kg N:P2O5 ha-1 + Inoculation with strain Vm M1) was highest but economically the treatment (20:50 kg N:P2O5 ha-1+Inoculation with strain Vm M1) with net return of Rs. 13618 ha-1and BCR of 2.52 was on top. © 2016, Pakistan Botanical Society. All rights reserved.

Khan J.,Arid Zone Research Center | Khan S.,Arid Zone Research Center | Khetran M.A.,Arid Zone Research Center | Amanullah,Arid Zone Research Center | And 4 more authors.
Pakistan Journal of Botany | Year: 2013

Tijaban-10 a drought tolerant wheat variety was developed by Arid Zone Research Centre for rainfed (Sailaba/Khushkaba) areas of Balochistan and has been released by Balochistan Seed Council in 2010. Tijaban-10 is a semi dwarf wheat variety with high tillering capacity, high yield, bold grain and improved drought tolerance. The variety posses high protein content (13.29 %), high wet gluten (29.91%) and dry gluten (10.04%). The yield potential of Tijaban-10 was tested (1997-2008) in different yield trials (PYT, AYT) at AZRC, Quetta, micro plot trials and national trials over multiple locations in rainfed areas of Balochistan and Pakistan. The variety showed stability and wide adoptability under prevalent biotic (Rust and Insect/pest) and abiotic (drought and cold) stress of highland Balochistan. The line was tested over wide range of locations with extreme variation (cold and drought) in rainfed/Sailaba areas of Balochistan (Quetta, Mastung, Killa Saifullah and Loralai). Tijaban-10 produced higher yield in National Uniform Wheat Yield Trial (NUWYT) conducted by National Coordinated Program (NARC) Islamabad during 2006-07 in rainfed areas of Pakistan. Tijaban-10 out yielded check cultivars (AZRI-96, Sariba-92 & Local White) during yield trials in different agro-ecological zones of highland Balochistan with an increase of 20-34% over check cultivars. Tijaban-10 also yielded higher in micro plot (2005-2007) yield trials and produced 25-50% increased yield as compared to check cultivars. Tijaban-10 produced 6 % higher grain yield in National Uniform Yield Trial (2006-07) than check genotypes in pooled analysis all over Pakistan with potential yield of 7000 kg ha-1. It showed 5MS-10MS susceptibility to yellow rust strain of highland Balochistan while it showed resistant to leaf rust when tested in natural conditions of rainfed areas. The variety possesses desirable quality characteristics such as better chapatti quality; improved protein (13.29%) and higher harvest index (30-38%). This variety showed high acceptability/popularity among the farming communities of Balochistan and a new addition in genetic diversity of germplasm/cultivars already available in rainfed areas of Balochistan with significant improvement in farmers yield.

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