Medenine, Tunisia
Medenine, Tunisia

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Abid M.,Institute of Arid Lands | Mansour E.,Institute of Arid Lands | Ben Khaled A.,Institute of Arid Lands | Bachar K.D.,Institute of Arid Lands | And 2 more authors.
Bulgarian Journal of Agricultural Science | Year: 2016

South Tunisian oases are characterized by arid environment in which drought is a major limiting factor for growth and crop production. In these conditions, understanding plant adaptation is required in order to preserve such regions from degradation. Four levels of water stress were applied to evaluate the effect of drought in three Medicago sativa populations (Chenini, Gannouch and Tebelbou): 25%; 50%; 75% and 100% of field capacity. Under water deficit conditions, leaves and roots exhibited a higher increase in proline, total soluble sugars, amino acids and potassium. A decrease in dry matter production was observed especially in alfalfa leaves. Chenini population showed the highest increase in different solutes, in contrary Tebelbou seems to be the most affected by drought as it showed lowest values. A close relationship was observed between proline and several osmolytes indicating that it is an important indicator of drought stress tolerance in alfalfa plants. © 2016, National Centre for Agrarian Sciences. All rights reserved.


Mansour E.,Institute of Arid Lands | Khaled A.B.,Institute of Arid Lands | Triki T.,Institute of Arid Lands | Abid M.,Institute of Arid Lands | And 2 more authors.
Journal of Agricultural Science and Technology | Year: 2015

Morphological and RAPD markers were used to investigate the genetic diversity among 21 accessions of pomegranate originating from South Eastern Tunisia. Thirteen morphological traits were studied and results showed significant differences for all morphological characters (P< 0.001). Clustering based on fruit traits, using Ward’s method, divided the accessions into three main groups. In RAPD analysis, 6 out of 15 employed random primers showed good amplification and polymorphism on pomegranate samples with a total of 63 bands, of which 56 were polymorphic. The lowest percentage of polymorphism (50%) was observed with TIBMBA-03 while the highest (50%) was observed with primer TIBMBB-03. According to Jaccard coefficient, the lowest (0.29) and highest (0.94) similarities were detected between genotypes. UPGMA clustering based on data from polymorphic RAPD bands resulted in three clusters at a similarity of 0.46. The Stress value for the nonmetric multidimensional scaling plot was 0.071, showing an excellent representation of the data. The comparison between groupings based on the fruit traits and RAPD data did not produce a significant correlation (r= -0.09). Using a stepwise linear regression, significant regressions were found between 13 morphological traits and 63 molecular markers revealing association between RAPD molecular markers and some traits. © 2015, Tarbiat Modares University. All rights reserved.

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