Arid Land Institute of Medenine

of Medenine, Tunisia

Arid Land Institute of Medenine

of Medenine, Tunisia

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Miladi H.,Laboratory of Analysis | Miladi H.,National School of Management | Ben Slama R.,Laboratory of Analysis | Mili D.,University of Sfax | And 3 more authors.
Journal of Chemistry | Year: 2013

The present study describes chemical composition as well as cytotoxic, antioxidant, and antimicrobial activities of winter savory Satureja montana L. essential oil (EO). The plant was collected from south France mountain, and its EO was extracted by hydrodistillation (HD) and analysed by gas chromatography/mass spectrometry (GC/MS). Thirty-two compounds were identified accounting for 99.85% of the total oil, where oxygenated monoterpenes constituted the main chemical class (59.11%). The oil was dominated by carvacrol (53.35%), γ-terpinene (13.54%), and the monoterpenic hydrocarbons p-cymene (13.03%). Moreover, S. montana L. EO exhibited high antibacterial activities with strong effectiveness against several pathogenic food isolated Salmonella spp. including S. enteritidis with a diameter of inhibition zones growth ranging from 21 to 51 mm and MIC and MBC values ranging from 0.39-1.56 mg/mL to 0.39-3.12 mg/mL, respectively. Furthermore, the S. montana L. EO was investigated for its cytotoxic and antioxidant activities. The results revealed a significant cytotoxic effect of S. montana L. EO against A549 cell line and an important antioxidant activity. These findings suggest that S. montana L. EO may be considered as an interesting source of components used as potent agents in food preservation and for therapeutic or nutraceutical industries. © 2013 Hanene Miladi et al.


Houta O.,Arid Land Institute of Medenine | Houta O.,University of Tunis | Akrout A.,Arid Land Institute of Medenine | Najja H.,Arid Land Institute of Medenine | And 2 more authors.
Journal of Essential Oil-Bearing Plants | Year: 2015

Crithmum maritimum L., a species original from France and cultivated in Tunisia arid zones. In this study, we interested to the chemical composition and biological activities of essential oils from different parts of Crithmum maritimum L.,. Theses oils were isolated by hydrodistillation and analyzed by GC and GC-MS. The antioxidant activity was evaluated by ABTS tests and the antibacterial activity was tested using the diffusion method. Yellow oils were obtained with yields ranging between 0.28 % and 3.60 %. Our results showed that essential oils of different plant parts were mainly composed by dillapiole (2.39 to 41.35 %), thymyl methyl ether (20.13 to 34.75 %), p-cymene (4.83 to 22.08 %) and γ-terpinene (22.54 to 43.29 %). Evaluation of antioxidant activity showed that the oils of flowers exhibited the most important antioxidant potential oils of flowers, leaves, seeds were the most active against Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus epidermidis, respectively. The essential oil of seeds was the most active against Staphylococcus aureus with an inhibition zone diameter of 18±0.5 mm. This study can contribute to provide useful data in understanding the impact of culture of Crithmum maritimum on chemical composition and biological effects of its essential oil and further studies should be conducted to achieve this goal. © 2015, Har Krishan Bhalla & Sons.


Teyeb H.,Arid Land Institute of Medenine | Teyeb H.,University of Monastir | Zouari S.,Arid Land Institute of Medenine | Douki W.,University of Monastir | And 2 more authors.
Zeitschrift fur Naturforschung - Section C Journal of Biosciences | Year: 2011

The essential oils obtained by hydrodistillation (Clevenger apparatus) from aerial parts of Astragalus gombiformis were analysed by gas chromatography coupled with mass spectrometry (GC/MS). This study showed that the A. gombiformis essential oils are complex mixtures of important natural compounds, which varied qualitatively and quantitatively between cultivated and wild plants and between phenological stages of development. All analysed oils are characterized by the constant presence of phytol, 6,10,14-trimethyl-2-pentadecanone, 4-terpineol, and γ-terpinene. This study is the first report on the chemical composition of essential oils from A. gombiformis and indicates that these oils should be more studied. © 2011 Verlag der Zeitschrift für Naturforschung, Tübingen.


Teyeb H.,University of Monastir | Teyeb H.,Arid Land Institute of Medenine | Zanina N.,University of Monastir | Neffati M.,Arid Land Institute of Medenine | And 2 more authors.
Turkish Journal of Biology | Year: 2012

Many species of the genus Astragalus have been long used in folk medicine because of their biological properties. The aim of this study was to investigate the antibacterial and cytotoxic activities of extracts from the leaves of wild Astragalus gombiformis Pomel. Antibacterial activity was assayed against several common human pathogenic bacterial strains using the paper disk-agar diff usion method. Cytotoxicity was measured against human lung carcinoma cells using the colorimetric MTT assay. The strongest cytotoxic activity against the human A549 lung epithelial carcinoma cell line was detected for dichloromethane extracts at 50 = 85 ±} 21.7 μg/mL. The best incubation time for this extract was 48 h. Most of the tested extracts were active against the tested bacterial strains. The methanol extract was found to be the most active against Pseudomonas aeruginosa and Salmonella typhimurium. Th is study is the first report on the biological activity of leaf extracts of Astragalus gombiformis with respect to antibacterial activity as well as cytotoxicity. © TUBITAK.


Teyeb H.,University of Monastir | Teyeb H.,Arid Land Institute of Medenine | Houta O.,Arid Land Institute of Medenine | Najjaa H.,Arid Land Institute of Medenine | And 4 more authors.
Zeitschrift fur Naturforschung - Section C Journal of Biosciences | Year: 2012

Extracts of aerial parts and roots of wild Astragalus gombiformis Pomel were tested for their antibacterial, antioxidant, and insecticidal activities and contents of phenolic compounds. Antibacterial activity was tested by the paper disk agar diffusion method and determination of the minimal inhibitor concentration. Among the tested extracts, three extracts (methanol, chloroform, and ethyl acetate) from aerial parts and two extracts (water, methanol) from roots exhibited diameters of inhibition zone equal or above 12 mm (at 150 μg/ disk) and minimal inhibitor concentrations ranging between 233 and 1250 μg/ml. Spectrophotometric and HPLC analyses showed that contents of both total polyphenols and flavonoids, as well as antioxidant activity were higher in the methanolic extract of aerial parts as compared to roots. No insecticidal activity of the extracts of the aerial parts was found against Culex pipiens. © 2012 Verlag der Zeitschrift für Naturforschung, Tübingen.


Lamari A.,Cytogenetic and Histology Laboratory | Teyeb H.,University of Monastir | Cheikh H.B.,Cytogenetic and Histology Laboratory | Douki W.,University of Monastir | Neffati M.,Arid Land Institute of Medenine
Journal of Essential Oil-Bearing Plants | Year: 2014

This study was conducted to elucidate the chemical composition of essential oil of Salvia officinalis L. cultivated in Tunisia and to evaluate the insecticidal activity of this oil. The essential oil was extracted by hydrodistillation from fresh aerial part and analysed by gas chromatography and gas chromatographymass spectrometry. The major components were αthujone (21 %), camphor (16.58 %), 1,8-cineole (14.57 %), viridiflorol (7.47 %), β-thujone (6.76 %), manool (5.34 %), and camphene (4.51 %). Insecticidal assays showed that the essential oil is active against Culex pipiens pipiens larvae. The LC50 (the 50 % lethal concentration) and LC90 (the 90 % lethal concentration) were 58.91 and 113 μg/ml, respectively. This study showed that S. officinalis L. has also an insecticidal potential through its essential oil content. © 2014 Har Krishan Bhalla & Sons.

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