Time filter

Source Type

Thomasz L.,Argentine National Atomic Energy Commission | Oglio R.,Argentine National Atomic Energy Commission | Rossich L.,Argentine National Atomic Energy Commission | Villamar S.,Argentine National Atomic Energy Commission | And 7 more authors.
Prostaglandins Leukotrienes and Essential Fatty Acids | Year: 2013

Background: IL-δ (5-hydroxy-6 iodo-8,11,14-eicosatrienoic delta lactone) an iodinated arachidonic acid (AA) derivative, is one of the iodolipids biosynthesized by the thyroid. Although IL-δ regulates several thyroid parameters such as cell proliferation and goiter growth it was found that this iodolipid inhibits the growth of other non thyroid cell lines. Objectives: To study the effect of IL-δ on cell proliferation and apoptosis in the colon cancer cell line HT-29. Results: Treatment with IL-δ reduced cell viability in a concentration-dependent manner: 1 μM 20%, 5 μM 25%, 10 μM 31%, 50 μM 47% and caused a significant decrease of PCNA expression (25%). IL-δ had pro-apoptotic effects, evidenced by morphological features of programmed cell death such as pyknosis, karyorrhexis, cell shrinkage and cell blebbing observed by fluorescence microscopy, and an increase in caspase-3 activity and in Bax/Bcl-2 ratio (2.5 after 3. h of treatment). Furthermore, IL-δ increased ROS production (30%) and lipid peroxidation levels (19%), suggesting that apoptosis could be a result of increased oxidative stress. A maximum increase in c-fos and c-jun protein expression in response to IL-δ was observed 1. h after initiation of the treatment. IL-δ also induced a tumour growth delay of 70% compared to the control group in NIH nude mice implanted with HT-29 cells. Conclusion: Our study shows that IL-δ inhibits cell growth and induces apoptosis in the colon cancer cell line, HT-29 and opens the possibility that IL-δ could be a potential useful chemotherapy agent. © 2013 Elsevier Ltd.


Thomasz L.,Argentine National Atomic Energy Commission | Oglio R.,Argentine National Atomic Energy Commission | Dagrosa M.A.,Argentine National Atomic Energy Commission | Krawiec L.,Argentine National Atomic Energy Commission | And 3 more authors.
Molecular and Cellular Endocrinology | Year: 2010

Background: Iodide has direct effects on thyroid function. Several iodinated lipids are biosynthesized by the thyroid and they were postulated as intermediaries in the action of iodide. Among them 6 iodo-δ-lactone (IL-δ) has been identified and proposed to play a role in thyroid autoregulation. The aim of this study was to compare the effect of iodide and IL-δ on several thyroid parameters. Methods: Thyroid bovine follicles were incubated with the different compounds during three days. Results: KI and IL-δ inhibited iodide uptake, total protein and Tg synthesis but only KI had an effect on NIS and Tg mRNAs levels. Both compounds inhibited Na+/K+ ATPase and deoxy-glucose uptake. As PAX 8, FOXE 1 and TITF1 are involved in the regulation of thyroid specific genes their mRNA levels were measured. While iodide inhibited the expression of the first two, the expression of TITF1 was stimulated by iodide and IL-δ had no effect on these parameters. Conclusion: These findings indicate that IL-δ reproduces some but not all the effects of excess iodide. These observations apply for higher micromolar concentrations of iodide while no such effects could be demonstrated at nanomolar iodide concentrations. © 2010 Elsevier Ireland Ltd.

Loading Argentine National Atomic Energy Commission collaborators
Loading Argentine National Atomic Energy Commission collaborators