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Pinilla I.,University of Zaragoza | Pinilla I.,Aragon Institute of Health science IIS Aragon | Izaguirre L.B.,Hospital Garcia Orcoyen | Gonzalvo F.J.,Complejo Hospitalario de Navarra | And 6 more authors.
The Scientific World Journal | Year: 2014

Purpose. To evaluate the dose effect of vitamin Kon wound healing mechanisms. Methods. Conjunctival fibroblasts were incubated for 24 hours. An artificial wound was made and the cells were incubated with fresh medium plus doses of vitamin Kto be tested. Wound repair was monitored at 0, 18, 24, and 48 hours. Proliferation was measured in actively dividing cells by [ 3H]thymidine uptake. Six different groups were tested: group 1/no drugs added, group 2/ethanol 0.1%, group 3/vitamin K1 mg/L, group 4/vitamin K2 mg/L, group 5/vitamin K4 mg/L, and group 6/vitamin K6 mg/L. Each experiment was carried out in triplicate and 4 times. Results. There were no differences among groups at the initial time. In vitro wound repair was slower in groups 4, 5, and 6. There were no differences between control and ethanol groups and between control and vitamin K1 mg/L groups. Fibroblast mitogenic activity was statistically decreased in all vitamin K groups; statistical differences were found among vitamin K1 mg/mL and higher doses too. In groups 5 and 6, cellular toxicity was presented. Conclusions. Vitamin Kis able to inhibit fibroblast proliferation. Vitamin K2 mg/L or higher doses inhibit wound healing repair, exhibiting cellular toxicity at 4 and 6 mg/L. © 2014 I. Pinilla et al.

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