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Memon A.A.,University Putra Malaysia | Wahid H.,University Putra Malaysia | Rosnina Y.,University Putra Malaysia | Goh Y.M.,University Putra Malaysia | And 2 more authors.
Animal Reproduction Science | Year: 2012

This study was conducted to determine the effect of antioxidants on standard semen parameters, lipid peroxidation and fertility of Boer goat semen after cryopreservation. Ejaculates from four bucks were collected, evaluated and pooled at 37. °C. The pooled semen was diluted with Tris citric acid fructose for washing. Semen samples, which were diluted with a Tris-based extender containing the antioxidant ascorbic acid (8.5. mg/ml), butylated hydroxytoluene (2. mM), cysteine (5. mM) and hypotaurine (10. mM) and an extender without antioxidant supplementation were cooled to 4. °C and frozen in 0.25 straws with programmable freezer and finally stored in liquid nitrogen. Data (10 replicates) were analyzed by one-way analysis of variance. Mean (±SEM) progressive motility was significantly higher in ascorbic acid than other supplement groups and control samples (P> 0.05). Best values were observed in ascorbic acid followed by BHT, cysteine, and hypotaurine. Antioxidant supplementation in extender showed significant (P< 0.05) better values than the control group for sperm membrane integrity, acrosome integrity and viability. The ability of antioxidants to reduce the lipid peroxidation (LPO) after freeze thawing was measured by the formation of malondialdehyde (MDA) using the thiobarbituric acid method. Results showed that addition of antioxidants significantly reduced the rate of LPO in comparison to control (P< 0.05). Ascorbic acid exhibited better values (1.27 ± 0.28), than butylated hydroxytoluene, cysteine and hypotaurine 1.32 ± 0.42, 2.27 ± 0.16 and 2.38 ± 0.17 respectively, which are significantly better than control (3.52 ± 0.54). Higher pregnancy rate was observed with ascorbic acid followed by butylated hydroxtolune, hypotaurine and cysteine. However, differences in the fertility rate were non-significant with hypotaurine, cysteine and control groups. © 2012 Elsevier B.V. Source


Memon A.A.,University Putra Malaysia | Wahid H.,University Putra Malaysia | Rosnina Y.,University Putra Malaysia | Goh Y.M.,University Putra Malaysia | And 3 more authors.
Journal of Animal and Veterinary Advances | Year: 2012

The objective of this study was to investigate whether pre freezing and post thawing (motility, morphology, viability, acrosome integrity and membrane integrity). Boer goat spermatozoa are affected by collecting in an Tris citric acid fructose extender supplemented with hypotaurine or cysteine. Semen was collected from four bucks into tubes containing 0 mL extender (Contro I), 2 mL extender without supplementation of antioxidants (Control II), 2 mL extender supplemented with hypotaurine 10 mM and 2 mL extender with cysteine 5 mm. No significant (p>0.05) improvement was observed by adding extender into collection tubes either supplemented or not with antioxidants before freezing and after freeze thawing. While a slight improvement was observed in motility, membrane integrity and acrosome integrity when 2 mL Tris citric acid fructose extender supplemented with either hypotaurine or cysteine. In conclusion, addition of extender in collection tubes either supplemented or not with antioxidants is not beneficial for Boer goat semen cryopreservation. © Medwell Journals, 2012. Source


Memon A.A.,University Putra Malaysia | Wahid H.,University Putra Malaysia | Rosnina Y.,University Putra Malaysia | Goh Y.M.,University Putra Malaysia | And 2 more authors.
Reproduction in Domestic Animals | Year: 2013

To improve the Boer goat semen quality during cryopreservation process, three experiments were carried out to investigate the effect of (i) different concentration of ascorbic acid supplementation (ii) rate of cooling with chilled semen characteristics and (iii) method of freezing on post-thaw Boer goat sperm using Tris-based extender. Ascorbic acid at 8.5mg/ml improved the sperm parameters (motility, integrity of membrane and acrosome, morphology and viability), compared to control in cooled samples (p<0.05). With regard to other concentrations and post-thawed parameters, ascorbic acid at 2.5-8.5mg/ml led to higher percentages of sperm motility and integrities of membrane and acrosome when compared to control (p<0.05). Slow cooling rises to higher percentages of sperm motility, acrosome integrity and viability, in comparison with fast cooling, in terms of cooled and frozen samples (p<0.05). Programmable freezing method produced the higher percentages of sperm motility, integrities of membrane and acrosome and viability when compared to the freezing method of polystyrene box during goat sperm freezing (p<0.05). In conclusion, chilled and post-thawed sperm quality of Boer goat was improved when a Tris-based extender supplemented with ascorbic acid was used at stages of different cooling rates and freezing methods. © 2012 Blackwell Verlag GmbH. Source


Naing S.W.,University Putra Malaysia | Wahid H.,University Putra Malaysia | Mohd Azam K.,Ar Raudhah Biotech Farm Sdn. Bhd | Rosnina Y.,University Putra Malaysia | And 6 more authors.
Animal Reproduction Science | Year: 2010

In order to improve Boer goat semen quality during cryopreservation process, the influence of sugar supplementation on semen characteristics of sperm were investigated. Three experiments were carried out to investigate the effect of (a) addition of two monosaccharides (fructose and glucose) and two disaccharides sugars (trehalose and sucrose) (b) sugar combination (fructose and trehalose, sucrose and trehalose, glucose and trehalose), and control (glucose without trehalose) (c) different concentrations of trehalose on cryopreservation using Tris based extender. The total motility, forward motility, viability, normal spermatozoa, acrosome integrity and membrane integrity were assessed subjectively. Differences were not detected among monosaccharides, but glucose increased (P<0.05) sperm forward motility in post-thaw goat semen compared to trehalose or sucrose supplementation. Semen quality did not differ (P>0.05) among disaccharide sugar supplementation. Combination of glucose and trehalose significantly improved the characteristics of Boer spermatozoa after cryopreservation (P<0.05). Supplementation of trehalose (198.24. mM) into the glucose extender significantly increased total motility, forward motility, live spermatozoa, acrosome integrity and membrane integrity following cryopreservation (P<0.05). In conclusion, glucose had the better ability to support Boer sperm motility and movement patterns. Combination of monosaccharide (glucose) and disaccharide (trehalose) improved semen quality following cryopreservation. Trehalose supplementation at the concentration of 198.24. mM to the glucose extender conferred the greater improvement of semen quality for Boer semen cryopreservation. © 2010 Elsevier B.V. Source


Goriman M.A.K.,Ar Raudhah Biotech Farm Sdn. Bhd | Bakar M.Z.A.,University Putra Malaysia | Thein M.,University of Veterinary Science, Yezin | Kyaw T.,University of Veterinary Science, Yezin | San M.M.,University of Veterinary Science, Yezin
Pertanika Journal of Tropical Agricultural Science | Year: 2011

Three experiments were carried out to improve semen quality during cryopreservation process. Total motility, forward motility, acrosome integrity, live spermatozoa, and normal spermatozoa were measured as semen quality. In Experiment 1, the effects of seminal plasma removal were analyzed by using two different extenders (GE and FE). The removal of seminal plasma gave higher and significant (P<0.05) effect in the total motility, forward motility, and live spermatozoa after cryopreservation. For two different extenders, however, the differences were not observed on the semen quality. In Experiment 2, three different washing solutions (namely, phosphate buffered saline, normal saline and Tris-based extender) were tested to evaluate the effects of semen quality after cryopreservation. Tris-based extender (TCG) conferred the highest (P<.05) sperm quality values in the total motility, forward motility, and live spermatozoa after cryopreservation. In Experiment 3, the effects of different centrifugation regimes (3000 × g for 3 min, 1600 × g for 10 min, 800 × g for 15 min) were evaluated on Boer semen quality. Semen quality parameters (namely, total motility, forward motility, acrosome integrity, and live spermatozoa) were significantly (P<.05) higher for cryopreserved spermatozoa centrifuged with 3000 × g for 3 min than the others. In conclusion, the removal of seminal plasma, washing solution TCG, and the use short-term centrifugation with a relative high g-force could contribute to the increased Boer semen quality after cryopreservation. © Universiti Putra Malaysia Press. Source

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