Aquaculture Center

São Paulo, Brazil

Aquaculture Center

São Paulo, Brazil
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Viadanna P.H.O.,University of Florida | Viadanna P.H.O.,Aquaculture Center | Miller-Morgan T.,Oregon State University | Peterson T.,Oregon State University | And 6 more authors.
Diseases of Aquatic Organisms | Year: 2017

Cyprinid herpesvirus 1 (CyHV1) infects all scaled and color varieties of common carp Cyprinus carpio, including koi. While it is most often associated with unsightly growths known as 'carp pox,' the underlying lesion (epidermal hyperplasia) can arise from a variety of disease processes. CyHV1-induced epidermal hyperplasia may occur transiently in response to water temperature, and thus histopathology cannot be used in isolation to assess CyHV1 infection status. To address this problem, here we describe a PCR assay targeted to the putative thymidine kinase gene of CyHV1. The PCR assay generates a 141 bp amplicon and reliably detects down to 10 copies of control plasmid DNA sequence (analytic sensitivity). The PCR does not cross-detect genomic DNA from cyprinid herpesvirus 2 and 3 (analytic specificity). The CyHV1 PCR effectively detected viral DNA in koi and common carp sampled from various locations in the UK, USA, Brazil, and Japan. Viral DNA was detected in both normal appearing and grossly affected epidermal tissues from koi experiencing natural epizootics. The new CyHV1 PCR provides an additional approach to histopathology for the rapid detection of CyHV1. Analysis of the thymidine kinase gene sequences determined for 7 PCR-positive carp originating from disparate geographical regions identified 3 sequence types, with 1 type occurring in both koi and common carp. © Inter-Research 2017.

Ibrahim F.S.,Aquaculture Center | Goddard J.S.,Sultan Qaboos University | Ambu Ali A.,Sultan Qaboos University | Rana K.J.,University of Stirling | Rana K.J.,Stellenbosch University
Journal of Applied Ichthyology | Year: 2010

The goldlined seabream Rhabdosargus sarba (Forskål, 1775) is farmed in several countries of the Western Pacific Rim and is a candidate species for aquaculture in the Gulf States of the Middle East. This study was undertaken to examine the onset and patterns of maturity in male goldlined seabream in support of the development of practical hatchery techniques. The testes of 337 male fish (0 + to 7 + , average FL 183 ± 20 to 315 ± 12 mm) collected from the Oman trap fishery (August 2001-April 2002; September 2002-2003) were classified into four stages, based on cell type (immature, developing, active or resting). Four spermatogenetic cell types were identified in the testes spermatogonia (types a and b), spermatocytes, spermatids and spermatozoa. Active testes appeared more regularly during the peak of the reproductive season in January and February. In March, resting testes were observed indicating the end of the reproductive season. There was clear evidence of protandry. Intersex gonads were observed in male fish from age 0 + to 7 + (33% at age 0 + to 75% at age 7 + with maximum 82% intersex frequency observed at age 5 + ). From the evidence presented, male fish age 1 + produced free-flowing sperm under slight abdominal pressure during the period November-March. © 2010 Blackwell Verlag, Berlin.

Silva-Gomes A.L.,Federal University of Amazonas | Gomes Coelho-Filho J.,Federal University of Amazonas | Viana-Silva W.,Federal University of Amazonas | Braga-Oliveira M.I.,Federal University of Amazonas | And 2 more authors.
Latin American Journal of Aquatic Research | Year: 2017

This study evaluates the impact of Neoechinorhynchus buttnerae on the productive and economic performance of “tambaqui” reared in ponds. Two fish farms were selected, one with infected fish (IF) and the other with non-infected fish (NIF). Biometry, age, initial weight and handling data were collected from each studied specimen. Our results show that IF presented lower weight, evident loss of body mass and body deformities, reflecting on a condition factor 300% smaller than NIF. Infection by acanthocephalans caused an impact bigger than 200% on growth and affected the gross income directly, resulting in a difference higher than 1000% between IF and NIF farms. We conclude that this impact could be even greater if we consider both income decrease and raising production costs due to the parasite infection. © Escuela de Ciencias del Mar. All rights reserved.

Dias D.C.,Aquaculture Center | Leonardo A.F.G.,Polo Regional Vale do Ribeira APTA | Tachibana L.,Fishery Institute APTA SAA | Correa C.F.,Polo Regional Vale do Ribeira APTA | And 3 more authors.
Journal of Applied Ichthyology | Year: 2012

Summary: The aim of the present study was to evaluate the influence of probiotics prepared from Bacillus subtilis (strain C-3102-Calsporin ®; Calpis, Tokyo, Japan) as a dietary supplement on several reproductive aspects of female 'matrinxã'(Brycon amazonicus), including hematological parameters, immunological characteristics and total lipid concentrations in muscle and liver tissue. The broodstock were kept in two ponds of 600m 2, at a density of 50 fish per pond, from March to November 2008. The fish were divided into two treatment groups: untreated control fish (T1), and fish fed with 10g of probiotics per kg of ration (T2). Fish were fed a diet containing 32% crude protein (CP), 7% crude fat (CF), and 6.5% ether extract (EE) twice daily at a rate of 1% of the total biomass per day during the coldest months (May through August) and 3% during the warm season (March, April and September through November). During the reproductive period, 20 individuals from each treatment group were selected for the experiments; two doses of crude carp pituitary extract were given to females in 10-h intervals, and one dose was given to the males at the same time the females were given the second dose. A total of 20 fish were used for the hematological analyses. After anesthetization, blood samples were withdrawn by caudal puncture to determine the total number of cells, differential and total leukocyte counts, thrombocyte count, hematocrit, hemoglobin rate and plasma cortisol and glucose levels. The hematimetric index mean corpuscular volume (VCM) and mean corpuscular hemoglobin concentration (MHCM) were calculated. Ten other 'matrinxãs' from the same groups were used to assess macrophage phagocytic activity and lipid levels. The results showed that females fed a probiotics-supplemented diet exhibited an increase in numbers of oocytes and, consequently, had higher rates of fertilization and hatching of larvae. Probiotic-treated fish also exhibited a significant increase in the phagocytic activity of macrophages, indicating an improvement in the immune system of breeders. Hematologic parameters were different in comparison to the time-zero group, and plasma cortisol and glucose levels were higher in the females. The total lipids levels in muscle and liver tissue were lower in B. amazonicus that received feed supplemented with probiotics. © 2011 Blackwell Verlag, Berlin.

Costa J.,Aquaculture Center | Freitas R.,Secretaria Executiva de Pesca e Aquicultura | Gomes A.L.,Federal University of Amazonas | Bernadino G.,Secretaria Executiva de Pesca e Aquicultura | And 2 more authors.
Latin American Journal of Aquatic Research | Year: 2016

The seeding rate is a factor that affects water quality, biological, physiological parameters, incidence of parasites and economic indicators. 72,000 fish of ± 1.9 ± 0.1 cm and 0.4 ± 0.01 g were planted in 12 ponds of 600 m2 in densities of 5, 10 and 15 fish m-2. The fish were fed twice a day until apparent satiation, with a commercial feed with 36% crude protein, for 56 days. The cost was determined based on the total production cost and total operational cost, showing unit values per hectare of water surface. Performance indicators were compared with ANOVA test with polynomial regression. The final number of fish showed a positive linear behavior, while the other performance parameters were not affected by density, thus suggesting that the pond’s maximum stocking density was not reached. On investment and production costs the most representative items were nursery building and fry acquisition, respectively, which denotes that increasing seeding density positively affected the production process, improving all the economic indicators. © 2016, Escuela de Ciencias del Mar. All rights reserved.

Mirheydari S.-M.,Islamic Azad University at Tehran | Matinfar A.,Aquaculture Center | Emadi H.,Islamic Azad University at Tehran
World Applied Sciences Journal | Year: 2014

The effects of feeding three Isolipidic and Isonitrogenous diets containing different dietary HUFA ratios (1, 2 and 3%) on gonadsomatic index (GSI), hepatosomatic index (HSI), number of mature females (N4), feed conversion ratio (FCR), instantaneous growth ratio (IGR), survival, ovary and hepatopancreas fatty acid composition of White leg shrimp (Litopenaeus vannamei) broodstock was compared with a fresh food diet in a 40-day feeding trial. No significant differences (at P>0.05) among treatments were observed for survival. The GSI and N4 were higher in 3% dietary HUFA and fresh food than those fed the rest treatments. Increasing Dietary HUFA to 30% led increasing GSI and N4, however the GSI affected only by higher dietary HUFA treatment (P<0.05). Shrimp fed diets containing 1% Dietary HUFA obtained significantly higher instantaneous growth rate, and feed conversion ratios than those fed the rest diets (P<0.05). The results suggest that each of 3%dietary HUFA and 1% Dietary HUFA may play specific important roles in reproduction and biologic performances of female broodstock L. vannamei respectively. © IDOSI Publications, 2014.

Sipauba-Tavares L.H.,São Paulo State University | Millan R.N.,São Paulo State University | Penariol I.C.,São Paulo State University | Penariol I.C.,Aquaculture Center
Limnetica | Year: 2015

The aim of this study was to investigate the effects of the aquatic macrophyte (Eichhornia crassipes) and periphyton communities on the neotropical fishponds water quality, and to determine the ability of such communities to remove potential pollutants from the fishponds, making them interesting tools for the maintenance of desirable water quality in aquaculture under continuous water flow. In general, the biological treatments used in this study showed a positive effect in reducing BOD5, orthophosphates, total phosphorus, conductivity and thermotolerant coliforms from the water column and also decreasing organic matter, Cu, Fe, Mn and Zn, from the sediment. The differences in physical and chemical properties of the water column and sediment between the treatments showed an improvement in the overall water quality when the treated fishponds are compared with the control fishpond. The best growth rate performance of Nile tilapia (Oreochromis niloticus) occurred in the fishpond with periphyton treatment. © Asociación Ibérica de Limnología, Madrid. Spain.

Salze G.,Virginia Polytechnic Institute and State University | Craig S.R.,Virginia Cobia Farms | Smith B.H.,University of Toronto | Smith E.P.,Virginia Polytechnic Institute and State University | Mclean E.,Aquaculture Center
Journal of Fish Biology | Year: 2011

The morphological development of larval cobia Rachycentron canadum from 3 days post hatch (dph) until weaning (27 dph) was examined using S.E.M. Two groups of fish were studied: a control group (CF), reared under standard feeding protocol, and a group in which prey items were enriched with supplemental taurine (4 g l-1 day-1; TF). TF fish grew faster (P < 0·001), attained greater size (mean ±s.e. 55·1 ± 1·5 v. 33·9 ± 1·0 mm total length) and had better survival (mean ±s.e. 29·3 ± 0·4 v. 7·1 ± 1·2 %) than CF fish. Canonical variance analysis confirmed findings with respect to differences in growth between the treatment groups with separation being explained by two cranial measurements. S.E.M. revealed that 3 dph larvae of R. canadum (in both groups) possess preopercular spines, superficial neuromasts on the head and body, taste buds in the mouth, an olfactory epithelium which takes the form of simple concave depressions, and primordial gill arches. Gill filaments start to form as early as 6 dph and lamellae buds are visible at 8 dph in both groups. In CF fish, the cephalic lateral line system continues its development at 12-14 dph with invagination of both supra- and infraorbital canals. At the same time, a thorn-like or acanthoid crest forms above the eye. At 14 dph, invaginations of the mandibular and preopercular canals are visible and around 22 dph enclosure of all cranial canals nears completion. In CF larvae, however, completely enclosed cranial canals were not observed within the course of the trial, i.e. 27 dph. In TF larvae, grooves of the cephalic lateral line system form 4 days earlier than observed in CF larvae of R. canadum (i.e. at 8 dph), with enclosure commencing at 16 dph, and completed by 27 dph. Along the flanks of 6 dph larvae of either treatment, four to five equally spaced neuromasts delineate the future position of the trunk lateral line. As myomeres are added to the growing larvae, new neuromasts appear such that at 16 dph a neuromast is associated with each myomere. By 27 dph, the trunk lateral line starts to invaginate in CF larvae, while it initiates closure in TF larvae. These findings elucidate important features of the larval development of R. canadum and show that dietary taurine supplementation benefits larval development, growth and survival in this species. Moreover, they suggest a conditional requirement for taurine in larval R. canadum. © 2011 The Authors. Journal of Fish Biology © 2011 The Fisheries Society of the British Isles.

Suloma A.,Cairo University | Mabroke R.S.,Cairo University | El-Haroun E.R.,Cairo University | El-Haroun E.R.,Aquaculture Center
Aquaculture International | Year: 2013

A growth trial was conducted to evaluate meat and bone meal (MBM) as a source of Phosphorus (P) for Nile tilapia fed with plant-based diets on growth, and the efficiency of P utilization. Four isonitrogenous and isoenergetic diets were formulated. A plant-based diet, deficient in P (0.45 % diet, no P added), was used as the basal diet. Three levels of MBM were substituted for cornstarch in the base diet to produce experimental diets containing MBM0.56, MBM0.67, or MBM0.78% P. These diets were fed (to apparent satiation) to Nile tilapia (initial body weight = 1.53 ± 0.01 g) for eight weeks. Weight gain (WG), specific growth rate (SGR), feed conversion ratio (FCR, feed:gain), whole-body P concentration (WBPC), protein retention (PR), and retained phosphorus (RP) increased significantly (P ≤ 0.05), with the increasing dietary P levels coming from MBM. Diets containing MBM0.78 produced significantly greater WG, SGR, WBPC, PR, and RP compared to other experimental diets (P ≤ 0.05). The linear regression analysis revealed a positive correlation between the WG, WBPC, RP, and dietary P levels coming from MBM of Nile tilapia. These results indicate that MBM has an additional value as a source of P and can serve as a potential source of supplemental P for Nile tilapia fed plant-protein-based diets. © 2012 Springer Science+Business Media B.V.

PubMed | Aquaculture Center and University of Extremadura
Type: Journal Article | Journal: Andrology | Year: 2015

In this study a Bayesian network (BN) has been built for the study of the objective motility of Tinca tinca spermatozoa (spz). Semen from eight 2-year-old sexually mature male tenchs was obtained and motility analyses were performed at 6-17, 23-34 and 40-51s after activation, using computer-assisted sperm analysis (CASA) software. Motility parameters rendered by CASA were treated with a two-step cluster analysis. Three well-defined sperm subpopulations were identified, varying the proportion of spermatozoa contained in each cluster with time and male. Cluster, cinematic and time variables were used to build the BN to study the probabilistic relationships among variables and how each variable influenced the final sperm classification into one of three predefined clusters. Both network structure and conditional probabilities were calculated based on the collected data set. Results shown that almost all the variables were directly or indirectly related to each other. By doing probabilistic inference we observed that the cluster distribution corresponded to the definition provided by the cluster analysis. Also, velocity and time variables determined the cluster to which each spermatozoon belonged with a high degree of accuracy. Thus, BNs can be applied in the study of sperm motility. The construction of a BN that include fertility data opens a new way to try to clarify the roles of motility and other sperm quality indicators in fertilization.

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