Aqua Green Technology Co.

Jeju, South Korea

Aqua Green Technology Co.

Jeju, South Korea
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Kang N.,Jeju National University | Ko S.-C.,Jeju National University | Samarakoon K.,Jeju National University | Samarakoon K.,Aqua Green Technology Co. | And 9 more authors.
Food Science and Biotechnology | Year: 2013

In this study, an antioxidative peptide was obtained by hydrolyzation of mideodeok (Styela clava) flesh tissue with various proteases and purified through gel filtration chromatography and reverse phage-HPLC (RPHPLC), and then antioxidant activity was investigated using electron spin resonance (ESR) spectrometer. Among the enzymatic hydrolysates, the peptic hydrolysate exhibited the highest antioxidant activity, and a strong antioxidant peptide was purified from the hydrolysate. The peptide sequence, Leu-Trp-His-Thr-His (692. 2 Da), was identified by quardruple time-of-flight electrospray ionization (QTOF ESI)-MS. This antioxidative peptide strongly scavenged peroxyl radical with the IC50 value of 39. 4 μM. © 2013 The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht.


Kang J.-I.,Jeju National University | Kim E.-J.,Jeju National University | Kim M.-K.,Jeju National University | Jeon Y.-J.,Aqua Green Technology Co. | And 5 more authors.
Marine Drugs | Year: 2013

This study was conducted to evaluate the promoting effect of Ishige sinicola, an alga native to Jeju Island, Korea, on hair growth. When vibrissa follicles were cultured in the presence of I. sinicola extract for 21 days, I. sinicola extract increased hair-fiber length. After topical application of I. sinicola extract onto the back of C57BL/6 mice, anagen progression of the hair shaft was induced. The I. sinicola extract significantly inhibited the activity of 5α-reductase. Treatment of immortalized vibrissa dermal papilla cells (DPCs) with I. sinicola extract resulted in increase of cell proliferation, which was accompanied by the increase of phospho-GSK3β level, β-catenin, Cyclin E and CDK2, whereas p27kip1 was down-regulated. In particular, octaphlorethol A, an isolated component from the I. sinicola extract, inhibited the activity of 5α-reductase and increased the proliferation of DPCs. These results suggest that I. sinicola extract and octaphlorethol A, a principal of I. sinicola , have the potential to treat alopecia via the proliferation of DPCs followed by the activation of β-catenin pathway, and the 5α-reductase inhibition. © 2013 by the authors; licensee MDPI.


Kang J.-I.,Jeju National University | Kim M.-K.,Jeju National University | Lee J.-H.,Jeju National University | Jeon Y.-J.,Jeju National University | And 7 more authors.
Marine Drugs | Year: 2017

In this study, we investigated the effect and mechanism of Undariopsis peterseniana, an edible brown alga, on hair growth. The treatment of vibrissa follicles with U. peterseniana extract ex vivo for 21 days significantly increased the hair-fiber lengths. The U. peterseniana extract also significantly accelerated anagen initiation in vivo. Moreover, we found that U. peterseniana extract was able to open the KATP channel, which may contribute to increased hair growth. The U. peterseniana extract decreased 5α-reductase activity and markedly increased the proliferation of dermal papilla cells, a central regulator of the hair cycle. The U. peterseniana extract increased the levels of cell cycle proteins, such as Cyclin D1, phospho(ser780)-pRB, Cyclin E, phospho-CDK2, and CDK2. The U. peterseniana extract also increased the phosphorylation of ERK and the levels of Wnt/β-catenin signaling proteins such as glycogen synthase kinase-3β (GSK-3β) and β-catenin. These results suggested that the U. peterseniana extract had the potential to influence hair growth by dermal papilla cells proliferation through the activation of the Wnt/β-catenin and ERK pathways. We isolated a principal of the U. peterseniana extract, which was subsequently identified as apo-9′-fucoxanthinone, a trichogenic compound. The results suggested that U. peterseniana extract may have a pivotal role in the treatment of alopecia. © 2017 by the authors. Licensee MDPI.


Kang M.-C.,Jeju National University | Kang S.-M.,Jeju National University | Ahn G.,Tokyo University of Technology | Kim K.-N.,Korea Basic Science Institute | And 6 more authors.
Environmental Toxicology and Pharmacology | Year: 2013

In this study, the hepatoprotective effect of dieckol on carbon tetrachloride (CCl4) induced hepatic damages in ICR mice liver was investigated. Mice were randomly divided into 4 groups such as saline treated (negative control), CCl4 treated (positive control), CCl4+dieckol (5mg/kg mouse) and CCl4+dieckol (25mg/kg mouse), respectively. The body weights and survival rates of mice, followed by dieckol treatments were significantly increased compared to the positive control. The level of GOT, GPT and MDA in the serum of the dieckol treated groups were reduced dose dependently than the control, significantly. The antioxidant enzymes including CAT, and GSH-px levels were increased significantly compared to the positive control. However, no significant differences were observed on hepatic histophathological analysis in dieckol treated groups dose dependently. Down-regulation of Bax and up-regulation of Bcl-xl protein expressions were observed in liver tissues of the dieckol administered groups. These results suggested that, dieckol can be developed as a therapeutic agent for liver disease by oxidative stress. © 2013 Elsevier B.V.


Kang M.-C.,Jeju National University | Kim K.-N.,Korea Basic Science Institute | Kang S.-M.,Jeju National University | Yang X.,Jeju National University | And 8 more authors.
Environmental Toxicology and Pharmacology | Year: 2013

In the present study, the protective effects of phlorotannins isolated from Ecklonia cava against ethanol-induced cell damage and apoptosis were investigated both in vitro and in vivo. Three phlorotannin compounds, namely phloroglucinol, eckol and dieckol, were successively isolated and identified from the extract. Dieckol showed the strongest protective effect against ethanol-induced cell apoptosis in Chang liver cells, with the lowest cytotoxicity. It was observed that dieckol reduced cell apoptosis through activation of Bcl-xL and PARP, and down-regulation of Bax and caspase-3 in Western blot analyses. In the in vivo study, the protective effect of ethanol induced by dieckol was investigated in a zebrafish model. The dieckol treated group scavenged intracellural reactive oxygen species and prevented lipid peroxidation and ethanol induced cell death in the zebrafish embryo. In conclusion, dieckol isolated from E. cava might possess a potential protective effect against ethanol-induced liver diseases. © 2013 Elsevier B.V.


Kang M.-C.,Jeju National University | Wijesinghe W.A.J.P.,Jeju National University | Lee S.-H.,Jeju National University | Kang S.-M.,Jeju National University | And 8 more authors.
Food and Chemical Toxicology | Year: 2013

In the present study, the attenuation of type II diabetes by dieckol, a phlorotannin derivative isolated from brown seaweed, Ecklonia cava was investigated in C57BL/KsJ-db/db, a type II diabetes mouse model. Dieckol was administered intraperitoneal injection at doses of 10 and 20. mg/kg body weight diabetes mice for 14 days. The blood glucose level, serum insulin level and body weight were significantly reduced in the dieckol administered group, compared to that of the saline administered group. Furthermore, reduced thiobarbituric acid reactive substraces (TBARS), as well as increased activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-px) in liver tissues were observed in the dieckol administered group. In addition, increased levels of the phosphorylation of AMPK and Akt were observed in the muscle tissues of the dieckol administered group in a Western blotting analysis. According to the findings of this study, it could be suggested that, dieckol can be developed as a therapeutic agent for type II diabetes. © 2012 Elsevier Ltd.


Manzoor Z.,Jeju National University | Mathema V.B.,Jeju National University | Chae D.,Jeju National University | Kang H.-K.,Jeju National University | And 4 more authors.
Bioscience, Biotechnology and Biochemistry | Year: 2013

Octaphlorethol A is a phenolic compound isolated from the marine alga Ishige foliacea. In the present study, we investigated the anti-inflammatory activity of octaphlorethol A in CpG-stimulated primary murine bone marrow-derived macrophages and dendritic cells. It exhibited anti-inflammatory activity by regulating the mitogen-activated protein kinase and NF-κB pathways.


Kang G.-J.,Jeju National University | Han S.-C.,Jeju National University | Koh Y.-S.,Jeju National University | Kang H.-K.,Jeju National University | And 2 more authors.
Biomolecules and Therapeutics | Year: 2012

Prostaglandin (PG) E2, the most abundant prostaglandin in the human body, is synthesized from arachidonic acid via the actions of cyclooxygenase (COX) enzymes. PGE2 exerts homeostatic, cytoprotective, inflammatory, and in some cases anti-inflammatory effects. Also, it has been reported that PGE2 is involved in hair growth. Diphlorethohydroxycarmalol (DPHC) is a phlorotannin compound isolated from the brown algae Ishige okamurae, with various biological activities in vitro and in vivo. In this study, the biological effect and mechanism of action of DPHC on prostaglandin synthesis in HaCaT human keratinocytes was examined. The results showed that, in these cells, DPHC significantly and dose-dependently induced PGE2 synthesis by increasing the protein and mRNA levels of COX-1 and COX-2. Interestingly, DPHC-induced COX-1 expression preceded that of COX-2. Also, while both rofecoxib and indomethacin inhibited PGE2 production, the latter was seems to be the more potent. From above results, we can expect that DPHC has some beneficial effects via increasing of PGE2 production. © 2012 The Korean Society of Applied Pharmacology.


Ko S.-C.,Jeju National University | Lee M.,Sungshin Women's University | Lee J.-H.,Jeju National University | Lee S.-H.,Konkuk University | And 3 more authors.
Environmental Toxicology and Pharmacology | Year: 2013

In this study, we assessed the potential inhibitory effect of 5 species of brown seaweeds on adipogenesis the differentiation of 3T3-L1 preadipocytes into mature adipocytes by measuring Oil-Red O staining. The Ecklonia cava extract tested herein evidenced profound adipogenesis inhibitory effect, compared to that exhibited by the other four brown seaweed extracts. Thus, E. cava was selected for isolation of active compounds and finally the three polyphenol compounds of phlorotannins were obtained and their inhibitory effect on adipogenesis was observed. Among the phlorotannins, dieckol exhibited greatest potential adipogenesis inhibition and down-regulated the expression of peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer-binding proteins (C/EBPα), sterol regulatory element-binding protein 1 (SREBP1) and fatty acid binding protein 4 (FABP4) in a dose-dependent manner. The specific mechanism mediating the effects of dieckol was confirmed by AMP-activated protein kinase (AMPK) activation. These results demonstrate inhibitory effect of dieckol compound on adipogenesis through the activation of the AMPK signal pathway. © 2013 Elsevier B.V.


Kang M.-C.,Jeju National University | Kim K.-N.,Korea Basic Science Institute | Wijesinghe W.A.J.P.,Jeju National University | Yang X.,Jeju National University | And 3 more authors.
Journal of Functional Foods | Year: 2014

In the present study, the protective effects of polyphenol extract from Ecklonia cava (EPE) against ethanol-induced cell damage and oxidative stress were investigated in vitro and in vivo. E. cava was selected among twenty marine brown algae due to less cytotoxicity and higher cell viability against the ethanol-induced cell damage. The EPE extract showed the protective effect against the ethanol-mediated apoptosis, which was investigated via nuclear staining with Hoechst 33342 and flow cytometry. Furthermore, the treated EPE extract on zebrafish model was improved the ethanol induced survival rate and attenuated, oxidative stress and cell death. As well this result indicated that EPE extract rendered the protective effect of EPE extract against ethanol induced oxidative stress in vitro and zebrafish model. © 2013 Elsevier Ltd.

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