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Khon Kaen, Thailand

Pinthong K.,Mahasarakham University | Gomontean B.,Mahasarakham University | Kongim B.,Mahasarakham University | Khakhong S.,Phuket Rajabhat University | And 2 more authors.
Cytologia | Year: 2013

We report the first cytogenetic comparison of four grouper genera from Thailand. Kidney cell samples were taken from the blueline hind (Cephalopholis formosa), humpback grouper (Cromileptes altivelis), orange-spotted grouper (Epinephelus coioides), and leopard coralgrouper (Plectropomus leopardus). The mitotic chromosome samples were prepared directly from the kidney cells. Conventional and Ag-NOR staining techniques were applied to stain the chromosomes. The results showed that diploid chromosome numbers of Ce. formosa, Cr. altivelis, E. coioides, and P. leopardus were 2n=48 for all species, and the fundamental numbers (NF) were 52, 52, 50, and 48, respectively. The presence of metacentric, submetacentric, acrocentric, and telocentric chromosomes were 2-0-2-44, 0-2-2-44, 0-0-2-46, and 0-0-0-48, respectively. After the Ag-NOR banding technique, one pair of nucleolar organizer regions (NORs) was observed on the short arm telomeric region of chromosome pair No. 2 in Ce. formosa, on the short arm telomeric region of chromosome pair No. 18 in Cr. altivelis, on the short arm telomeric region of chromosome pair No. 20 in E. coioides, and on the long arm subcentromeric region of chromosome pair No. 20 in P. leopardus. The karyotype formula could be deduced as Ce. formosa (2n=48): L 2 a+L28 t+M16 t+S2 m; Cr. altivelis (2n=48): L 2 a+L24 t+M2 sm+M8 t+S2 1 E. coioides (2n=48): L28 t+M2 a+M 16 t+S2 t; and P. leopardus (2n=48): L24 t+M24 t. © 2013 The Japan Mendel Society. Source


Khrueanet W.,Khon Kaen University | Supiwong W.,Applied Taxonomic Research Center | Tumpeesuwan C.,Mahasarakham University | Tumpeesuwan S.,Mahasarakham University | And 2 more authors.
Cytologia | Year: 2013

We report the first chromosome analysis and localization of the nucleolar organizer region of the land snail Sarika resplendens (Philippi 1846) in Thailand. The mitotic and meiotic chromosome preparations were carried out by directly taking samples from the ovotestis. Conventional and Ag-NOR staining techniques were applied to stain the chromosomes. The results showed that the diploid chromosome number of S. resplendens is 2n=66 and the fundamental number (NF) is 132. The karyotype has the presence of six large metacentric, two large submetacentric, 26 medium metacentric, and 32 small metacentric chromosomes. After using the Ag-NOR banding technique, one pair of nucleolar organizer regions (NORs) was observed on the long arm subtelomeric region of chromosome pair 11. We found that during metaphase I, the homologous chromosomes show synapsis, which can be defined as the formation of 33 ring bivalents, and 33 haploid chromosomes at metaphase II as diploid species. The karyotype formula of S. resplendens could be deduced as: (2n=66): L6 m +L 2 sm+M26 m+S32 m. © 2013 The Japan Mendel Society. Source


Wangwasit K.,Applied Taxonomic Research Center | Chantaranothai P.,Applied Taxonomic Research Center
Phytotaxa | Year: 2015

Hedyotis puffii is described and illustrated based on material from Thailand. The new species differs from H. tavoyensis by its obtuse angled stems, usually with (0­­ ̶ )1 ̶ 2 glands on the calyx margin and the sinus of the calyx that has no interposed subulate teeth. The conservation status of this new taxon is vulnerable (VU D2). In addition the new combination Hedyotis laotica is made and a lectotype is designated. © 2015 Magnolia Press Source


Phimphan S.,Applied Taxonomic Research Center | Tanomtong A.,Applied Taxonomic Research Center | Jumrusthanasan S.,Applied Taxonomic Research Center | Supiwong W.,Applied Taxonomic Research Center | And 2 more authors.
Cytologia | Year: 2013

The present study reports the first finding of the NOR (nucleolar organizer region) polymorphism and chromosome analysis in the John's snapper (Lutjarus johnii) from Thailand. Kidney cell samples were taken from four male and four female fish. Mitotic chromosome preparations were conducted using the standard squash technique, as well as directly from kidney cells. Metaphase spreads were performed on microscopic slides and then air-dried. Conventional and Ag-NOR banding techniques were applied to stain the chromosome. The results showed that the diploid chromosome number of L. johnii was 2n=48, and the fundamental number (NF) was 48 in both male and female. Karyotpes were present as 28 large telocentric, 16 medium telocentric, and 4 small telocentric chromosomes. No strange-sized chromosomes related to sex were observed. The results indicated that the long arm subcentromeric of the telocentric chromosome pair 1 showed clearly observable NORs (satellite chromosomes). In addition, a heteromorphism of one female had different-sized NORs in chromosome pair 1 (lalb), while three females and four males had equal-sized NORs in chromosome pair 1 with a homomorphism (lala). The karyotype formula for L. johnii is as follows: 2n (diploid) 48=Lt28+Mt16+S 14. © 2013 The Japan Mendel Society. Source


Tanomtong A.,Applied Taxonomic Research Center | Khunsook S.,Applied Taxonomic Research Center | Boonhan P.,Applied Taxonomic Research Center | Kaewmad P.,Mahasarakham University | And 2 more authors.
Cytologia | Year: 2013

This is the first karyological analysis of the hoary bamboo rat (Rhizomys pruinosus) from the Nongbualamphu, Nongkhai, and Loei Provinces in northeast Thailand. Conventional staining, GTG-, CBG-, and Ag-NOR banding, and high-resolution analysis were carried out on standard whole blood T-lymphocyte cultures from six specimens of R. pruinosus from three localities. The results showed that 2/7=50 and the fundamental number is 100 in both sexes. The autosomes consisted of 12 large acrocentric, 4 medium submetacentric, 6 medium acrocentric, 6 small metacentric, and 20 small acrocentric chromosomes. The X chromosome is the largest metacentric chromosome, while the Y chromosome is a small acrocentric chromosome. A multiple sex chromosome system of the X 1Y,X2Y/X1X2 type was found in R. pruinosus, which is the first description in the subfamily Rhizomyinae. From GTG-banding and high-resolution techniques, the numbers of bands and locations in R. pruinosus are determined to be 234 and 280, respectively, and each chromosome pair could be clearly differentiated. CBG-banding shows C-positive (dark band) on the centromere of all autosomes. However, C-negative (light band) was observed on the Y chromosome. The results demonstrated that chromosome pair 3 had an interstitial large band on the long arm near the telomere. In addition, the short arms near the telomere of chromosome pairs 4, 7, and 10 had clear nucleolar organizer regions (NORs). This is the first report on the natural polymorphism of NORs in bamboo rats, and it indicates the presence of heteromorphism of chromosome pair 10 (10a10b) in all males and females. There are NORs in 10b, but not in 10a. © 2013 The Japan Mendel Society. Source

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