Applied Taxonomic Research Center

Khon Kaen, Thailand

Applied Taxonomic Research Center

Khon Kaen, Thailand
SEARCH FILTERS
Time filter
Source Type

Kaewmad P.,Mahasarakham University | Tanomtong A.,Applied Taxonomic Research Center | Kaewboribut T.,Applied Taxonomic Research Center | Wonkaonoi W.,Mahasarakham University | And 2 more authors.
Cytologia | Year: 2013

This research was the first karyological analysis of the black crowned crane (Balearica pavonina) and the scaly-breasted munia (Lonchura punctulata). Two specimens of B. pavonina (one male and one female) and two specimens of L. punctulata (one male and one female) were obtained from Nakhon Ratchasima Zoo, Thailand. After using the standard peripheral blood lymphocyte culture method at 41°C for 72 h in the presence of colchicine, the metaphase spreads were performed on microscopic slides and air-dried. A conventional staining technique was applied to stain the chromosomes. The results showed that the diploid chromosome number of B. pavonina is 2n=80. The chromosomes have two sizes; 11 pairs of macrochromosomes and 29 pairs of microchromosomes were observed. The macrochromosomes consist of 14 metacentric, 6 submetacentric, and sex chromosomes. The Z chromosome is a medium metacentric chromosome and the W chromosome is a small metacentric chromosome. The diploid chromosome number of L. punctulata is 2n=12. The chromosomes have two sizes; 10 pairs of macrochromosomes and 25 pairs of microchromosomes were observed. The macrochromosomes consist of 2 metacentric, 4 submetacentric, 2 acrocentric, 10 telocentric, and sex chromosomes. The Z chromosome is a small metacentric chromosome and the W chromosome is the smallest metacentric chromosome. © 2013 The Japan Mendel Society.


Pinthong K.,Mahasarakham University | Gomontean B.,Mahasarakham University | Kongim B.,Mahasarakham University | Khakhong S.,Phuket Rajabhat University | And 2 more authors.
Cytologia | Year: 2013

We report the first cytogenetic comparison of four grouper genera from Thailand. Kidney cell samples were taken from the blueline hind (Cephalopholis formosa), humpback grouper (Cromileptes altivelis), orange-spotted grouper (Epinephelus coioides), and leopard coralgrouper (Plectropomus leopardus). The mitotic chromosome samples were prepared directly from the kidney cells. Conventional and Ag-NOR staining techniques were applied to stain the chromosomes. The results showed that diploid chromosome numbers of Ce. formosa, Cr. altivelis, E. coioides, and P. leopardus were 2n=48 for all species, and the fundamental numbers (NF) were 52, 52, 50, and 48, respectively. The presence of metacentric, submetacentric, acrocentric, and telocentric chromosomes were 2-0-2-44, 0-2-2-44, 0-0-2-46, and 0-0-0-48, respectively. After the Ag-NOR banding technique, one pair of nucleolar organizer regions (NORs) was observed on the short arm telomeric region of chromosome pair No. 2 in Ce. formosa, on the short arm telomeric region of chromosome pair No. 18 in Cr. altivelis, on the short arm telomeric region of chromosome pair No. 20 in E. coioides, and on the long arm subcentromeric region of chromosome pair No. 20 in P. leopardus. The karyotype formula could be deduced as Ce. formosa (2n=48): L 2 a+L28 t+M16 t+S2 m; Cr. altivelis (2n=48): L 2 a+L24 t+M2 sm+M8 t+S2 1 E. coioides (2n=48): L28 t+M2 a+M 16 t+S2 t; and P. leopardus (2n=48): L24 t+M24 t. © 2013 The Japan Mendel Society.


Khrueanet W.,Khon Kaen University | Supiwong W.,Applied Taxonomic Research Center | Tumpeesuwan C.,Mahasarakham University | Tumpeesuwan S.,Mahasarakham University | And 2 more authors.
Cytologia | Year: 2013

We report the first chromosome analysis and localization of the nucleolar organizer region of the land snail Sarika resplendens (Philippi 1846) in Thailand. The mitotic and meiotic chromosome preparations were carried out by directly taking samples from the ovotestis. Conventional and Ag-NOR staining techniques were applied to stain the chromosomes. The results showed that the diploid chromosome number of S. resplendens is 2n=66 and the fundamental number (NF) is 132. The karyotype has the presence of six large metacentric, two large submetacentric, 26 medium metacentric, and 32 small metacentric chromosomes. After using the Ag-NOR banding technique, one pair of nucleolar organizer regions (NORs) was observed on the long arm subtelomeric region of chromosome pair 11. We found that during metaphase I, the homologous chromosomes show synapsis, which can be defined as the formation of 33 ring bivalents, and 33 haploid chromosomes at metaphase II as diploid species. The karyotype formula of S. resplendens could be deduced as: (2n=66): L6 m +L 2 sm+M26 m+S32 m. © 2013 The Japan Mendel Society.


Tanomtong A.,Applied Taxonomic Research Center | Khunsook S.,Applied Taxonomic Research Center | Boonhan P.,Applied Taxonomic Research Center | Kaewmad P.,Mahasarakham University | And 2 more authors.
Cytologia | Year: 2013

This is the first karyological analysis of the hoary bamboo rat (Rhizomys pruinosus) from the Nongbualamphu, Nongkhai, and Loei Provinces in northeast Thailand. Conventional staining, GTG-, CBG-, and Ag-NOR banding, and high-resolution analysis were carried out on standard whole blood T-lymphocyte cultures from six specimens of R. pruinosus from three localities. The results showed that 2/7=50 and the fundamental number is 100 in both sexes. The autosomes consisted of 12 large acrocentric, 4 medium submetacentric, 6 medium acrocentric, 6 small metacentric, and 20 small acrocentric chromosomes. The X chromosome is the largest metacentric chromosome, while the Y chromosome is a small acrocentric chromosome. A multiple sex chromosome system of the X 1Y,X2Y/X1X2 type was found in R. pruinosus, which is the first description in the subfamily Rhizomyinae. From GTG-banding and high-resolution techniques, the numbers of bands and locations in R. pruinosus are determined to be 234 and 280, respectively, and each chromosome pair could be clearly differentiated. CBG-banding shows C-positive (dark band) on the centromere of all autosomes. However, C-negative (light band) was observed on the Y chromosome. The results demonstrated that chromosome pair 3 had an interstitial large band on the long arm near the telomere. In addition, the short arms near the telomere of chromosome pairs 4, 7, and 10 had clear nucleolar organizer regions (NORs). This is the first report on the natural polymorphism of NORs in bamboo rats, and it indicates the presence of heteromorphism of chromosome pair 10 (10a10b) in all males and females. There are NORs in 10b, but not in 10a. © 2013 The Japan Mendel Society.


Phimphan S.,Applied Taxonomic Research Center | Tanomtong A.,Applied Taxonomic Research Center | Jumrusthanasan S.,Applied Taxonomic Research Center | Supiwong W.,Applied Taxonomic Research Center | And 2 more authors.
Cytologia | Year: 2013

The present study reports the first finding of the NOR (nucleolar organizer region) polymorphism and chromosome analysis in the John's snapper (Lutjarus johnii) from Thailand. Kidney cell samples were taken from four male and four female fish. Mitotic chromosome preparations were conducted using the standard squash technique, as well as directly from kidney cells. Metaphase spreads were performed on microscopic slides and then air-dried. Conventional and Ag-NOR banding techniques were applied to stain the chromosome. The results showed that the diploid chromosome number of L. johnii was 2n=48, and the fundamental number (NF) was 48 in both male and female. Karyotpes were present as 28 large telocentric, 16 medium telocentric, and 4 small telocentric chromosomes. No strange-sized chromosomes related to sex were observed. The results indicated that the long arm subcentromeric of the telocentric chromosome pair 1 showed clearly observable NORs (satellite chromosomes). In addition, a heteromorphism of one female had different-sized NORs in chromosome pair 1 (lalb), while three females and four males had equal-sized NORs in chromosome pair 1 with a homomorphism (lala). The karyotype formula for L. johnii is as follows: 2n (diploid) 48=Lt28+Mt16+S 14. © 2013 The Japan Mendel Society.


Supiwong W.,Applied Taxonomic Research Center | Tanomtong A.,Applied Taxonomic Research Center | Jumrusthanasan S.,Applied Taxonomic Research Center | Khakhong S.,Phuket Rajabhat University | And 2 more authors.
Cytologia | Year: 2012

A first karyotype analysis and chromosomal characteristic of nucleolar organizer regions (NORs) of the common sheathfish, Micronema apogon (Bleeker 1985), from the Chao Praya, Chi, and Song karm basins (3 populations), Thailand, were studied. Kidney cell samples were taken from 10 male and 10 female fish. The mitotic chromosome preparations were conducted by taken directly from kidney, spleen and/or gill cells. Conventional and Ag-NOR staining techniques were applied to stain the chromosomes. The results showed that diploid chromosomes number of M. apogon was 2n=64, and the fundamental numbers (NF) were 108 in both sexes. The chromosome types consist of 10 large metacentric, 12 large submetacentric, 4 large acrocentric, 2 large telocentric, 8 medium metacentric, 8 medium submetacentric, 2 medium acrocentric, 8 medium telocentric and 10 small telocentric chromosomes. No strange size chromosomes related to sex were observed. In chromosome pair 3, the region adjacent to the telomere of the short arm showed clearly observable secondary constriction/NORs. The karyotype formula for M. apogon could be deduced as: In (diploid) 64=L m 10+Lsm 12+La 4+Lt 2+Mm 8+M sm 8+Ma 2+Mt 8+St 10 ©2012 The Japan Mendel Society.


Supiwong W.,Applied Taxonomic Research Center | Tanomtong A.,Applied Taxonomic Research Center | Jumrusthanasan S.,Applied Taxonomic Research Center | Khakhong S.,Phuket Rajabhat University | And 2 more authors.
Cytologia | Year: 2013

The standardized karyotype and idiogram of the titan triggerfish, Balistoides viridescens (Bloch & Schneider 1801) from Phuket Province, Thailand, were obtained from the present study. Kidney cell samples were taken from five male and five female fish. The mitotic chromosome preparations were conducted directly from the kidney cells. Conventional staining and Ag-NOR banding techniques were applied to stain the chromosomes. The results indicated that the diploid chromosome number of B. viridescens was 2n=44, and the fundamental number (NF) was 60 in both sexes. The chromosome types consist of 2 large metacentric, 4 large acrocentric, 2 large telocentric, 8 medium acrocentric, 10 medium telocentric, 2 small acrocentric, and 16 small telocentric chromosomes. No strange-sized chromosomes related to sex was observed. The region adjacent to the subtelomeric of short arm of chromosome pair 3 showed clearly observable secondary constriction/NORs. The karyotype formula for B. viridescens is as follows: 2n (diploid) 44=Lm 2+L4 n+Lt 2+M n 8+Mt 10+Sa 2+St 16. © 2013 The Japan Mendel Society.


Wangwasit K.,Applied Taxonomic Research Center | Chantaranothai P.,Applied Taxonomic Research Center
Phytotaxa | Year: 2015

Hedyotis puffii is described and illustrated based on material from Thailand. The new species differs from H. tavoyensis by its obtuse angled stems, usually with (0­­ ̶ )1 ̶ 2 glands on the calyx margin and the sinus of the calyx that has no interposed subulate teeth. The conservation status of this new taxon is vulnerable (VU D2). In addition the new combination Hedyotis laotica is made and a lectotype is designated. © 2015 Magnolia Press

Loading Applied Taxonomic Research Center collaborators
Loading Applied Taxonomic Research Center collaborators