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Raaman N.,University of Madras | Mahendran B.,University of Madras | Mahendran B.,University of Guelph | Jaganathan C.,University of Madras | And 2 more authors.
World Journal of Microbiology and Biotechnology | Year: 2010

The tannase production by Paecilomyces variotii was confirmed by high performance thin layer chromatography (HPTLC), and substrate specificity of the tannase was determined by zymogram analysis in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). A clear band of activity observed after electrophoresis of culture filtrate in non-denaturing gels indicated the production of extracellular tannase by P. varoitii. HPTLC analysis revealed that gallic acid was the enzymatic degradation product of tannic acid during the fermentation process. The optimum condition for tannase production was at 72 h of incubation in shaking condition and addition of 1. 5% tannic acid, 1% glucose and 0. 2% sodium nitrate at temperature of 35°C and pH of 5-7. The production of extracellular tannase from Paecilomyces variotii was investigated under optimized conditions in solid-state fermentation (SSF), submerged fermentation (SmF) and liquid surface fermentation (LSF) processes. The maximum extracellular tannase production was obtained within 60 h of incubation under SSF followed by SmF and LSF. © Springer Science+Business Media B.V. 2009. Source


Raaman N.,University of Madras | Mahendran B.,University of Madras | Mahendran B.,University of Guelph | Jaganathan C.,University of Madras | And 2 more authors.
World Journal of Microbiology and Biotechnology | Year: 2012

The chromium (CrIII and CrVI) removal capability of Rhizobium leguminosarum was checked by estimating the amount of chromium in the medium before and after inoculation. To determine the efficiency of R. leguminosarum in removal of chromium, the influence of physical and chemical parameters such as temperature, pH and different concentrations (0. 1-1. 0 mM) of trivalent (CrIII) and hexavalent (CrVI) chromium were studied. The chromium removal in aqueous solution by different size of active and inactivated biomass and immobilized cells of R. leguminosarum in a packed-bed column was also carried out. Results showed that in a medium containing up to 0. 5 mM concentration of both CrIII and CrVI, R. leguminosarum showed optimal growth. The maximum chromium removal was at pH 7. 0 and 35°C. Active biomass removed 84. 4 ± 3. 6% of CrIII and 77. 3 ± 4. 3% of CrVI in 24 h of incubation time. However, inactivated biomass removed maximum chromium after 36 h of incubation. Immobilized bacterial cells in a packed-bed column removed 86. 4 ± 1. 7% of CrIII and 83. 8 ± 2. 2% of CrVI in 16 and 20 h of incubation time, respectively. © 2011 Springer Science+Business Media B.V. Source

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