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Moriya K.,Applied Molecular Bioscience | Tsubota T.,Japan National Institute of Agrobiological Science | Ishibashi N.,Applied Molecular Bioscience | Yafune A.,Applied Molecular Bioscience | And 5 more authors.
Insect Molecular Biology | Year: 2010

The lipid modifications which occur on Bombyx mori Ras proteins BmRas1, BmRas2 and BmRas3 were studied by metabolic labelling in an insect cell-free protein synthesis system and in a baculovirus expression system, using specific inhibitors of protein prenylation and protein palmitoylation. In addition, the subcellular localization of BmRas proteins was examined using EGFP fusion proteins of constitutively active forms of BmRas proteins transiently expressed in Sf9 cells. As a result, it was revealed that the three B. mori Ras proteins BmRas1, BmRas2 and BmRas3 are neither farnesylated nor palmitoylated but are geranylgeranylated for localization to the plasma membrane of insect cells. Thus, the mechanism of membrane binding of insect Ras proteins is quite different from that reported for mammalian Ras proteins. © 2009 The Royal Entomological Society.

Ariyoshi T.,Applied Molecular Bioscience | Yoshinaga K.,Yamaguchi University | Koizumi K.,Applied Molecular Bioscience | Fujii H.,Yamaguchi University | And 3 more authors.
Heterocycles | Year: 2010

Aldol condensation of 2,3-diformyl-1-azaazulene with acetone, methoxyacetone, and benzylacetone in aq. NaOH solution gave corresponding 11-azacyclohept[a]azulen-3(3H)-one (2a), 2-methoxy-derivative (2b) and 2-benzyl-derivative (2d) in good yields. Hydrolysis of 2b using hot HBr gave 2-hydroxy-II-azacyclohept[α]azulen-3(3H)-one (2f). Acetylation of 2f gave 2-acetoxy-11-azacyclohept[α]azulen-3(3H)-one (2g). Reaction of the compounds (2a, 2b, 2f) with diethyl malonate in Ac2O under heating gave 1,9(11bH)-11 b-azaazuleno[ 1,2,3-cd]azulenedione derivatives (4a, 4b, 4f). Compounds (2b, 2f) showed weak cytotoxic activity against HeLa S3 cells. © 2010 The Japan Institute of Heterocyclic Chemistry.

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