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Khaloo P.,Tehran University of Medical Sciences | Sadeghi B.,Tehran University of Medical Sciences | Ostadhadi S.,Tehran University of Medical Sciences | Norouzi-Javidan A.,Tehran University of Medical Sciences | And 3 more authors.
Biomedicine and Pharmacotherapy | Year: 2016

Major depressive disorder is disease with high rate of morbidity and mortality. Stressful events lead to depression and they can be used as a model of depression in rodents. In this study we aimed to investigate whether lithium modifies the stressed-induced depression through blockade of opioid receptors in mice. We used foot shock stress as stressor and forced swimming test (FST), tail suspension test (TST) and open field test (OFT) to evaluation the behavioral responses in mice. We also used naltrexone hydrochloride (as opioid receptor antagonist), and morphine (as opioid receptor agonist). Our results displayed that foot-shock stress significantly increased the immobility time in TST and FST but it could not change the locomotor behavior in OFT. When we combined the low concentrations of lithium and naltrexone a significant reduction in immobility time was seen in the FST and TST in comparison with control foot-shock stressed group administered saline only. Despite the fact that our data showed low concentrations of lithium, when administered independently did not significantly affect the immobility time. Also our data indicated that concurrent administration of lithium and naltrexone had no effect on open field test. Further we demonstrated that simultaneous administration of morphine and lithium reverses the antidepressant like effect of active doses of lithium. Our data acclaimed that we lithium can augment stressed-induced depression and opioid pathways are involved in this action. © 2016

Ardeshirylajimi A.,Applied Cell Sciences | Azadian E.,Stem Cell Technology Research CenterTehranIran
Journal of Cellular Physiology | Year: 2017

Nowadays, tissue engineering by using stem cells in combination with scaffolds and bioactive molecules has made significant contributions to the regeneration of damaged bone tissues. Since the usage of bioactive molecules including, growth factors to induce differentiation is safety limited in clinical applications, and it has also been previously observed that extremely low frequency pulsed electromagnetic fields (PEMF) can be effective in the enhancement of proliferation rate and osteogenic differentiation of stem cells, the aim of this study was investigating the osteoinductive potential of PEMF in combination with Poly(caprolactone) (PCL) nanofibrous scaffold. To achieve this aim, Adipose-derived mesenchymal stem cells (ADSCs) isolated and characterized and then osteogenic differentiation of them was investigated after culturing on the surface of PCL scaffold under treatments of PEMF, PEMF plus osteogenic medium (OM) and OM. Analysis of common osteogenic markers such as Alizarin red staining, ALP activity, calcium content and four important bone-related genes in days of 7, 14, and 21 confirmed that the effects of PEMF on the osteogenic differentiation of ADSCs are very similar to the effects of osteogenic medium. Thus, regarding the immunological concerns about the application of bioactive molecules for tissue engineering, PEMF could be a good alternative for osteogenic medium. Although, results were showed a synergetic effect for simultaneous application of PEMF and PCL scaffold in the osteogenesis process of ADSCs. Taking together, ADSCs-seeded PCL nanofibrous scaffold in combination with PEMF could be a great option for use in bone tissue engineering applications. © 2017 Wiley Periodicals, Inc.

Ostadhadi S.,Tehran University of Medical Sciences | Ahangari M.,National University of Ireland | Nikoui V.,Tehran University of Medical Sciences | Norouzi-Javidan A.,Tehran University of Medical Sciences | And 5 more authors.
Biomedicine and Pharmacotherapy | Year: 2016

Lamotrigine is an anticonvulsant agent that shows clinical antidepressant properties. The aim of the present study was to investigate the involvement of N-methyl- d-aspartate (NMDA) receptors and nitric oxide-cyclic guanosine monophosphate (NO-cGMP) synthesis in possible antidepressant-like effect of lamotrigine in forced swimming test (FST) in mice. Intraperitoneal administration of lamotrigine (10 mg/kg) decreased the immobility time in the FST (P < 0.01) without any effect on locomotor activity in the open-field test (OFT), while higher dose of lamotrigine (30 mg/kg) reduced the immobility time in the FST (P < 0.001) as well as the number of crossings in the OFT. Pretreatment of animals with NMDA (75 mg/kg), l-arginine (750 mg/kg, a substrate for nitric oxide synthase [NOS]) or sildenafil (5 mg/kg, a phosphodiesterase [PDE] 5 inhibitor) reversed the antidepressant-like effect of lamotrigine (10 mg/kg) in the FST. Injection of l-nitroarginine methyl ester (l-NAME, 10 mg/kg, a non-specific NOS inhibitor), 7-nitroindazole (30 mg/kg, a neuronal NOS inhibitor), methylene blue (20 mg/kg, an inhibitor of both NOS and soluble guanylate cyclase [sGC]), or MK-801 (0.05 mg/kg), ketamine (1 mg/kg), and magnesium sulfate (10 mg/kg) as NMDA receptor antagonists in combination with a sub-effective dose of lamotrigine (5 mg/kg) diminished the immobility time of animals in the FST compared with either drug alone. None of the drugs produced significant effects on the locomotor activity in the OFT. Based on our findings, it is suggested that the antidepressant-like effect of lamotrigine might mediated through inhibition of either NMDA receptors or NO-cGMP synthesis. © 2016 .

Khan M.I.,Tehran University of Medical Sciences | Ostadhadi S.,Tehran University of Medical Sciences | Zolfaghari S.,Applied Cell Sciences | Ejtemaei Mehr S.,Tehran University of Medical Sciences | And 2 more authors.
Neuroscience Letters | Year: 2016

In the current study, the involvement of N-methyl-d-aspartate receptor (NMDAR) and nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) system in the antidepressant-like effects of baclofen was evaluated by using animal model in forced swimming test. Followed by an open field test for the evaluation of locomotor activity, the immobility time for mice in force swimming test was recorded. Only the last four min was analyzed. Administration of Baclofen (0.5 and 1 mg/kg, i.p.) reduced the immobility interval in the FST. Prior administration of l-arginine (750 mg/kg, i.p.,) a nitric oxide synthase substrate or sildenafil (5 mg/kg, i.p.) a phosphodiesterase 5 into mice suppressed the antidepressant-like activity of baclofen (1 mg/kg, i.p.).Co-treatment of 7-nitroindazole (50 mg/kg, i.p.,) an inhibitor of neuronal nitric oxide synthase, L-NAME (10 mg/kg, i.p.,) a non-specific inhibitor of nitric oxide synthase or MK-801 (0.05 mg/kg, i.p.) an NMDA receptor antagonist with subeffective dose of baclofen (0.1 mg/kg, i.p.), reduced the immobility time in the FST as compared to the drugs when used alone. Co-administrated of lower doses of MK-801 (0.01 mg/kg) or l-NAME (1 mg/kg) failed to effect immobility time however, simultaneous administration of these two agents in same dose with subeffective dose of baclofen (0.1 mg/kg, i.p.), minimized the immobility time in the FST. Thus, our results support the role of NMDA receptors and l-arginine-NO-GMP pathway in the antidepressant-like action of baclofen. © 2015 Elsevier Ireland Ltd.

Ostadhadi S.,Tehran University of Medical Sciences | Khan M.I.,Tehran University of Medical Sciences | Norouzi-Javidan A.,Tehran University of Medical Sciences | Chamanara M.,Tehran University of Medical Sciences | And 3 more authors.
Brain Research Bulletin | Year: 2016

Topiramate (TPM) is an agent primarily used in the treatment of epilepsy. Using mice model of forced swimming test (FST) the current study was basically aimed to investigate the influence of TPM on depression by inhibiting NMDA receptor and nitric oxide-cGMP production. When TPM was administered in a dose of 20 and 30 mg/kg by i.p. route it reduced the immobility time during FST. However this effect of TPM (30 mg/kg, i.p.) in the FST was abolished when the mice were pretreated either with NMDA (75 mg/kg, i.p.), or l-arginine (750 mg/kg, i.p. NO precursor), or sildenafil (5 mg/kg, i.p. Phosphodiesterase 5 inhibitor). The immobility time in the FST was reduced after administration of L-NAME (10 mg/kg, i.p, a non-specefic NOS inhibitor), 7-nitoinidazol (30 mg/kg, i.p. a nNOS inhibitor) or MK-801 (0.05 mg/ kg, i.p, a NMDA receptor antagonist) in combination with a subeffective dose of TPM (10 mg/kg, i.p.) as compared with single use of either drug. Co-administrated of lower doses of MK-801 (0.01 mg/kg) or L-NAME (1 mg/kg) failed to effect immobility time. However, simultaneous administration of these two agents in the same doses with subeffective dose of TPM (10 mg/kg, i.p.), reduced the immobility time during FST. None of these drugs were found to have a profound effect on the locomotor activity per se during the open field test.Taken together, our data demonstrates that TPM exhibit antidepressant-like effect which is accomplished either due to inhibition of NMDA receptors or NO-cGMP production. © 2016 Elsevier Inc.

Atia T.A.,Applied Cell Sciences
Kaohsiung Journal of Medical Sciences | Year: 2017

Apoptosis is an interactive and dynamic biological process involved in all phases of embryogenesis. We aimed to study the effect of placental apoptosis on recurrent miscarriage (RM). Placental tissue samples were collected from 40 women with RM (study group) and 30 women with sporadic spontaneous abortion (control group). Samples were prepared and stained immunohistochemically with markers for both the apoptotic protein (p53) and anti-apoptotic Bcl-2 antibodies. Our results showed that expression of the apoptotic (p53) protein was significantly increased in the placental tissues of the RM group (p = 0.003). By contrast, the expression of anti-apoptotic (Bcl-2) antibodies was significantly increased in the placental tissues of the control group (p = 0.025). We concluded that placental apoptosis plays a crucial role in pregnancy continuation. However, increased p53 expression in placental tissue in early pregnancy could negatively affect pregnancy continuation. © 2017.

Machado L.C.,University of Sao Paulo | Pelegati V.B.,Applied Cell Sciences | Oliveira A.L.,University of Sao Paulo
Journal of Supercritical Fluids | Year: 2016

Supercritical CO2 was studied for impregnation or encapsulation of essential oils in modified starches via Particle from Gas Saturated Solutions or Suspensions (PGSS). Modified starches were choose as function of its low cost. The advantage of this method over conventional encapsulation that use modified starch via spray drier refers to the low temperatures used and absence of water in the process. Modified starch presents hydrophobic elements and this molecules present amphiphilic character. Usually it is employed in the encapsulation of essences as wall material with excellent volatiles retention due to its polar and nonpolar interface. Considering its hydrophobic characteristics, interactions between the modified starch and supercritical CO2 occurred, resulting in two different structural interactions of the limonene and modified starch in the PGSS (50 °C and 60 °C at 100 bar and 120 bar). When hydrous ethanol was used in the suspension, impregnation occurred and, when anhydrous ethanol was used, encapsulation occurred. Analysis of particle morphology via scanning electron and confocal microscopy, thermo-oxidative characterization by differential scanning calorimetry and determination of microencapsulated limonene via gas chromatography coupled to mass spectrometry indicated limonene microencapsulation and impregnation occurred despite the highly solubility of limonene in supercritical CO2. The retention of limonene by Purity Gum Ultra® was 86% when encapsulated and, 53% when impregnated, similar values to those obtained in conventional microencapsulation methods via a spray drier or via PGSS-drying. © 2015 Elsevier B.V. All rights reserved.

Adur J.,National University of Entre Rios | Adur J.,Applied Cell Sciences | Carvalho H.F.,Applied Cell Sciences | Cesar C.L.,Applied Cell Sciences | Casco V.H.,National University of Entre Rios
Cancer Informatics | Year: 2014

This work reviews the most relevant present-day processing methods used to improve the accuracy of multimodal nonlinear images in the detection of epithelial cancer and the supporting stroma. Special emphasis has been placed on methods of non linear optical (NLO) microscopy image processing such as: second harmonic to autofluorescence ageing index of dermis (SAAID), tumor-associated collagen signatures (TACS), fast Fourier trans-form (FFT) analysis, and gray level co-occurrence matrix (GLCM)-based methods. These strategies are presented as a set of potential valuable diagnostic tools for early cancer detection. It may be proposed that the combination of NLO microscopy and informatics based image analysis approaches described in this review (all carried out on free software) may represent a powerful tool to investigate collagen organization and remodeling of extracellular matrix in carcinogenesis processes. © the authors.

Silva J.A.,University of Campinas | Ferrucci D.L.,University of Campinas | Peroni L.A.,University of Campinas | Abrahao P.G.,University of Campinas | And 5 more authors.
Journal of Cellular Physiology | Year: 2012

Molecular mechanisms responsible for periodontal disease (PD) and its worsening in type 1 Diabetes Mellitus (DM1) remain unknown. Cytokine profile and expression levels of collagenases, Mmp14, and tissue inhibitors were determined, as were the numbers of neutrophils and macrophages in combined streptozotocin-induced DM1 and ligature-induced PD models. Increased IL-23 (80-fold) and Mmp8 expression (25-fold) was found in DM1. Ligature resulted in an IL-1β/IL-6 profile, increased expression of Mmp8, Mmp13, and Mmp14 (but not Mmp1), and transient expression of Timp1 and Reck in non-diabetics. PD in DM1 involved IL-1β (but not IL-6) and IL-23/IL-17, reduced IL-6 and IL-10, sustained Mmp8 and Mmp14, increased Mmp13 and reduced Reck expression in association with 20-fold higher counts of neutrophils and macrophages. IL-23 and Mmp8 expression are hallmarks of DM1. In association with the IL-1/IL-6 (Th1) response in PD, one found a secondary IL-17 (Th17) pathway in non-diabetic rats. Low IL-6/TNF-α suggest that the Th1 response was compromised in DM1, while IL-17 indicates a prevalence of the Th17 pathway, resulting in high neutrophil recruitment. Mmp8, Mmp13, and Mmp14 expression seems important in the tissue destruction during PD in DM1. PD-associated IL-1/IL-6 (Th1), IL-10, and Reck expression are associated with the acute-to-chronic inflammation transition, which is lost in DM1. In conclusion, IL-23/IL-17 are associated with the PD progression in DM1. © 2011 Wiley Periodicals, Inc.

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