Applied Biochemistry and Biotechnology Center Biotehnol

Bucharest, Romania

Applied Biochemistry and Biotechnology Center Biotehnol

Bucharest, Romania

Time filter

Source Type

Emanuel V.,University of Bucharest | Emanuel V.,Applied Biochemistry and Biotechnology Center Biotehnol | Diana P.,University of Bucharest | Ionela A.,University of Bucharest | And 7 more authors.
Romanian Biotechnological Letters | Year: 2010

The strains Lactobacillus paracasei IL2 and Lactobacillus plantarum IL3 were identified, through the usage of MicroLog system. It was established that glucose is the glucide through which the maximum quantity of exopolysaccharides is obtained, namely 201 mg/L, by using MRS. The other glucides tested were lactose and sucrose. The quantity of glucose at which the maximum quantity of exopolysaccharides is obtained was 10%, resulting 240 mg/L, at 48 hours of fermentation. The capacity of biosynthesis was also tested at different values of pH and temperature, resulting that the maximum activity is obtained at 37°C and pH 7. © 2010 University of Bucharest.


Emanuel V.,Applied Biochemistry and Biotechnology Center Biotehnol | Emanuel V.,University of Bucharest | Adrian V.,Applied Biochemistry and Biotechnology Center Biotehnol | Adrian V.,University of Bucharest | And 2 more authors.
Journal of Medicinal Plants Research | Year: 2011

The aim of this study was to obtain a freeze-dried extract from Issopus officinalis with an important antioxidant and antimicrobial effect. The extract was obtained in a fluidized bed solid extractor and the optimal values of phenols, flavonoids and the highest reduction power were determined when using 75% ethanol as solvent, the antioxidant activity ranging between 90 to 95%. In case of the freeze-dried product, the maximum antioxidant activity was registered when using 0.5 mg/ml powder and the results were optimal as well in case of determining flavonoids, phenols and the reducing power. The antimicrobial effect of the extract and of the freeze-dried product were quantified by using the Colony Quant software on the strains Escherichia coli CBAB 2, Bacillus cereus CMGB 215, Listeria innocua CMGB 218. An inhibition area of maximum 2 cm resulted when using 75% ethanol as solvent. The optimal inhibitory effect of the freeze-dried product of around 1.7 cm was determined by a 0.5 mg/ml concentration. © 2011 Academic Journals.

Loading Applied Biochemistry and Biotechnology Center Biotehnol collaborators
Loading Applied Biochemistry and Biotechnology Center Biotehnol collaborators