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Khandelwal P.,Applied and Investigative Metabolomics Group | Stryker S.,Applied and Investigative Metabolomics Group | Chao H.,Discovery Chemistry and Discovery Biology | Aranibar N.,Applied and Investigative Metabolomics Group | And 5 more authors.
Journal of Lipid Research | Year: 2014

A method is described that allows noninvasive identifi cation and quantitative assessment of lipid classes present in sebaceous excretions in rodents. The method relies on direct high-fi eld proton NMR analysis of common group lipid protons in deuterated organic solvent extracts of fur. Extracts from as little as 15 mg of fur from rat, mouse, and hamster provided acceptable results on a 600 MHz NMR equipped with a cryogenically cooled protonobserve probe. In rats, sex- and age-related differences in lipid composition are larger than differences in fur collected from various body regions within an individual and much larger than interanimal differences in age- and sexmatched specimens. The utility of this method to noninvasively monitor drug-induced sebaceous gland atrophy in rodents is demonstrated in rats dosed with a stearoyl-CoA desaturase 1 (SCD1) inhibitor. In this model, a 35% reduction in sebum lipids, extracted from fur, was observed. Finally, structural elucidation of cholesta-7,24-dien-3 -ol ester as the most prominent, previously unidentifi ed sebum sterol ester in male Syrian hamsters is described. The utility of this method for drug and cosmetic safety and effi cacy assessment is discussed. -Khandelwal, P., S. Stryker, H. Chao, N. Aranibar, R. M. Lawrence, M. Madireddi, W. Zhao, L. Chen, and M. D. Reily. 1 H NMR-based lipidomics of rodent fur: species-specifi c lipid profi les and SCD1 inhibitor-related dermal toxicity. J. Lipid Res . 2014. 55: 1366-1374. © 2014 by the American Society for Biochemistry and Molecular Biology, Inc.

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