Richter A.,University of Bremen |
Lubbing M.,University of Bremen |
Lubbing M.,Leibniz University of Hanover |
Frank H.,AplaGen GmbH |
And 5 more authors.
European Cells and Materials | Year: 2011
In previous research, it was shown that recombinant HMGA2 protein enhances the proliferation of porcine chondrocytes grown in vitro, opening up promising applications of this embryonic architectural transcription factor for tissue engineering, such as in cartilage repair. In this paper, we describe the development and analyses of two synthetic fragments comprising the functional AT-hook motifs of the HMGA2 protein, as well as the nuclear transport domain. They can be synthesised up to large scales, while eliminating some of the problems of recombinant protein production, including unwanted modification or contamination by the expression hosts, or of gene therapy approaches such as uncontrolled viral integration and transgene expression even after therapy. Application of one of these peptides onto porcine hyaline cartilage chondrocytes, grown in in vitro monolayer cell culture, showed a growth-promoting effect similar to that of the wild type HMGA2 protein. Furthermore, it also promoted cell growth of adult adipose tissue derived stem cells. Due to its proliferation inducing function and vast availability, this peptide is thus suitable for further application and investigation in various fields such as tissue engineering and stem cell research.
AplaGen GmbH | Date: 2009-06-16
Peptides, peptide conjugates for scientific use; chemical compositions containing peptides or peptide conjugates for use in industry and science. Biochemical preparations, namely, peptides, peptide conjugates and compositions containing peptide or peptide conjugates for medical and veterinary use. Scientific research and development; industrial research in the field of medicine, biology and science.
AplaGen GmbH | Date: 2005-05-23
Pharmaceutical and veterinary preparations, namely peptides or peptide conjugates and compositions comprising peptides or peptide conjugates for the treatment of renal insufficiency, blood disorders, namely anemia, and for the stimulation of stem cells. Scientific and industrial design in the field of blood disorders, preferably anemia.
AplaGen GmbH | Date: 2006-01-31
Chemicals used in industry and science, namely synthetic peptides and proteins used as raw materials for the manufacture of pharmaceuticals, veterinary preparations and kits; synthetic peptides and proteins for use in the manufacture of pharmaceuticals and veterinary preparations for the detection and treatment of cancer, inflammatory diseases, immunodeficiency syndrome and infertility; synthetic peptides and proteins, namely, recombinant cytokines and antibodies. Pharmaceuticals and veterinary preparations, namely those comprised of synthetic peptides or proteins as raw materials; pharmaceuticals and veterinary preparations for the treatment and detection of cancer, inflammatory diseases, immunodeficiency syndrome and infertility. Scientific and industrial research in the field of cancer, inflammatory diseases, immuno-deficiency syndrome and infertility; scientific and industrial research in the area of synthetic peptides, recombinant cytokines and antibodies.
AplaGen GmbH | Date: 2010-08-31
Chemicals used in industry and science. Pharmaceutical preparations for prolongation of plasma circulation of pharmaceutical substances; pharmaceutical preparations for infusion therapy; pharmaceutical preparations for clinical nutrition; veterinary preparations for prolongation of plasma circulation of pharmaceutical substances; veterinary preparations for infusion therapy; veterinary preparations for clinical nutrition; dietetic food adapted for medical use; plasters for medical purposes; disinfectants for hygienic purposes and medical instruments. Scientific research; technological services, namely, synthesis of chemical substances and pharmaceutically active substances and of their precursors; research in the field of chemistry, pharmacy and pharmacology; molecular design of pharmaceutically active substances and their precursors; chemical analysis; product research.