Apis Flora Industrial e Comercial LTDA

Ribeirão Preto, Brazil

Apis Flora Industrial e Comercial LTDA

Ribeirão Preto, Brazil

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Batista B.L.,University of Sao Paulo | da Silva L.R.S.,Federal University of Goais | Rocha B.A.,University of Sao Paulo | Rodrigues J.L.,Federal University of Vales do Jequitinhonha and Mucuri | And 5 more authors.
Food Research International | Year: 2012

Multi-element analysis of honey samples was carried out with the aim of developing a reliable method of tracing the origin of honey. Forty-two chemical elements were determined (Al, Cu, Pb, Zn, Mn, Cd, Tl, Co, Ni, Rb, Ba, Be, Bi, U, V, Fe, Pt, Pd, Te, Hf, Mo, Sn, Sb, P, La, Mg, I, Sm, Tb, Dy, Sd, Th, Pr, Nd, Tm, Yb, Lu, Gd, Ho, Er, Ce, Cr) by inductively coupled plasma mass spectrometry (ICP-MS). Then, three machine learning tools for classification and two for attribute selection were applied in order to prove that it is possible to use data mining tools to find the region where honey originated. Our results clearly demonstrate the potential of Support Vector Machine (SVM), Multilayer Perceptron (MLP) and Random Forest (RF) chemometric tools for honey origin identification. Moreover, the selection tools allowed a reduction from 42 trace element concentrations to only 5. © 2012 Elsevier Ltd.


Brancalion A.P.S.,University of Sao Paulo | Oliveira R.B.,University of Sao Paulo | Sousa J.P.B.,University of Sao Paulo | Groppo M.,University of Sao Paulo | And 4 more authors.
Urological Research | Year: 2012

Copaifera langsdorffii Desf.commonly known as "copaíba", produce a commercially valuable oil-resin that is extensively used in folk medicine for anti-inflammatory, antimicrobial and antiseptic purposes. We have found the hydroalcoholic extract of this plant leaf has the potential to treat urolithiasis, a problem affecting ∼7% of the population. To isolate the functional compounds C. langsdorffii leaves were dried, ground, and macerated in a hydroalcoholic solution 7:3 to produce a 16.8% crude extract after solvent elimination. Urolithiasis was induced by introduction of a calcium oxalate pellet (CaOx) into the bladders of adult male Wistar rats. The treated groups received the crude extract by oral gavage at 20 mg/kg body weight daily for 18 days. Extract treatment started 30 days after CaOx seed implantation. To monitor renal function sodium, potassium and creatinine concentrations were analyzed in urine and plasma, and were found to be in the normal range. Analyses of pH, magnesium, phosphate, calcium, uric acid, oxalate and citrate levels were evaluated to determine whether the C. langsdorffii extract may function as a stone formation prevention agent. The HPLC analysis of the extract identified flavonoids quercitrin and afzelin as the major components. Animals treated with C. langsdorffii have increased levels of magnesium and decreased levels of uric acid in urinary excretions. Treated animals have a significant decrease in the mean number of calculi and a reduction in calculi mass. Calculi taken from extract treated animals were more brittle and fragile than calculi from untreated animals. Moreover, breaking calculi from untreated animals required twice the amount of pressure as calculi from treated animals (6.90 ± 3.45 vs. 3.00 ± 1.51). The extract is rich in flavonoid heterosides and other phenolic compounds. Therefore, we hypothesize this class of compounds might contribute significantly to the observed activity. © Springer-Verlag 2012.


De Mello Costa A.R.,Apis Flora Industrial e Comercial LTDA | Marquiafavel F.S.,Apis Flora Industrial e Comercial LTDA | De Oliveira Lima Leite Vaz M.M.,Apis Flora Industrial e Comercial LTDA | Rocha B.A.,Apis Flora Industrial e Comercial LTDA | And 4 more authors.
Journal of Thermal Analysis and Calorimetry | Year: 2011

Quercetin is a flavonoid very well studied and has already entered clinical trials emerging as prospective anticancer drug candidate. In addition, quercetin has being reported to its free-radical scavenging activity and suggests potential uses for the prevention and treatment of pathologies as atherosclerosis, chronic inflammation, and others. However, quercetin is sparingly soluble in water, which may be responsible for its limited absorption upon oral administration. The solid dispersion of quercetin with polyvinylpyrrolidone Kollidon® 25 (PVP K25) suggests an interesting way to increase quercetin solubility, antioxidant activity, and consequently bioavailability. Then, the purpose of this study was to prepare solid dispersions of quercetin with PVP K25 and evaluate their thermal characterization, antioxidant activity and quercetin improvement solubility. For this purpose, quercetin-PVP K25 solutions were dried and quercetin-PVP K25 solids were obtained. The formation of quercetin-PVP K25 solid dispersion was evaluated by solubility studies, powder X-ray diffraction (XRD), fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), thermogravimetry (TG), and antioxidant activity. It was observed that PVP K25 was able to provide quercetin clear aqueous solutions and that quercetin solubility was increased in a PVP K25 concentration dependent manner, improving solubility even 436-fold the pure quercetin. The results obtained with XRD, FT-IR, DSC, and TG demonstrated possible quercetin-PVP K25 solid dispersion formation. Besides, the antioxidant activity of the quercetin-PVP K25 solid dispersions dissolved in aqueous solution and pure quercetin dissolved in methanol showed IC50 value of 0.61 ± 0.03 and 1.00 ± 0.02 μg/mL, respectively, demonstrating that the solid dispersions presented a significant increase in antioxidant activity (P < 0.05). Putting results together, it was possible to conclude there was the formation of quercetin-PVP K25 solid dispersion. © Akadémiai Kiadó, Budapest, Hungary 2010.


Hori J.I.,University of Sao Paulo | Zamboni D.S.,University of Sao Paulo | Carrao D.B.,Apis Flora Industrial e Comercial Ltda | Goldman G.H.,University of Sao Paulo | And 3 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2013

Propolis extracts have gained the attention of consumers and researchers due to their unique chemical compositions and functional properties such as its anti-inflammatory activity. Recently, it was described a complex that is also important in inflammatory processes, named inflammasome. The inflammasomes are a large molecular platform formed in the cell cytosol in response to stress signals, toxins, and microbial infections. Once activated, the inflammasome induces caspase-1, which in turn induces the processing of inflammatory cytokines such as IL-1β and IL-18. So, to understand inflammasomes regulation becomes crucial to treat several disorders including autoinflammatory diseases. Since green propolis extracts are able to regulate inflammatory pathways, this work purpose was to investigate if this extract could also act on inflammasomes regulation. First, the extract was characterized and it demonstrated the presence of important compounds, especially Artepillin C. This extract was effective in reducing the IL-1β secretion in mouse macrophages and this reduction was correlated with a decrease in activation of the protease caspase-1. Furthermore, we found that the extract at a concentration of 30 g/mL was not toxic to the cells even after a 18-hour treatment. Altogether, these data indicate that Brazilian green propolis (EPP-AF) extract has a role in regulating the inflammasomes. © 2013 Juliana I. Hori et al.


Rocha B.A.,Apis Flora Industrial e Comercial LTDA | Rodrigues M.R.,Apis Flora Industrial e Comercial LTDA | Bueno P.C.P.,Apis Flora Industrial e Comercial LTDA | De Mello Costa-Machado A.R.,Apis Flora Industrial e Comercial LTDA | And 4 more authors.
Journal of Thermal Analysis and Calorimetry | Year: 2012

The propolis produced in Southeastern Brazil is known as green propolis (BGP) because of its color and the most important plant source is Baccharis dracunculifolia. Several authors reported biological activities such as antiulcer, anti-inflammator, antimutagenic, antifungal/antibacterial, antileishmanial/antiplasmodial for the BGP. For this reason, BGP has been extensively employed in food and beverages, thus helping improve health and preventing diseases. Some authors related that the biological activities of BGP are mostly due to its high levels of prenylated ρ-coumaric acids derivatives, mainly artepillin C. The inclusion complex between Brazilian green propolis (BGP) with hydroxypropyl-β-cyclodextrin (HP-β-CD) was prepared and its characterization was investigated by different analytical techniques (X-ray diffraction, Fourier transform infrared spectroscopy, and thermogravimetry) and suggesting that propolis was molecularly dispersed in the HP-β-CD matrix. The increasing solubility of chemical constituents was determined using quantitation methods for total flavonoids and polyphenols. Furthermore, it was developed a method for the quantitation and identification of the main compounds by high-performance liquid chromatography in order to evaluate the increasing water solubility of each constituent in aqueous BGP extract (aromadendrin, isosakuranetin, and artepillin C). The antioxidant activity was evaluated by chemical assay 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging. © 2011 Akadémiai Kiadó, Budapest, Hungary.


Oliveira R.B.D.,University of Sao Paulo | Coelho E.B.,University of Sao Paulo | Rodrigues M.R.,Apis Flora Industrial e Comercial Ltda. | Costa-Machado A.R.D.M.,University of Sao Paulo | And 4 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2013

The potential of the Copaifera langsdorffii leaves extract to prevent stone formation was analyzed by means of an ethylene glycol (EG) animal model of nephrolithiasis and an in vitro crystallization assay. Different doses of the C. langsdorffii leaves extract were administered to rats treated with EG. Urine biochemical parameters were quantified. CaOx deposits count and analysis of osteopontin expression were conducted on kidneys fixed in formalin. The in vitro assay was performed by turbidimetry. Phytochemical analyses of the extract were accomplished by HPLC-UV-DAD, and several compounds were isolated. C. langsdorffii leaf extract was able to avoid stone formation. The number of deposits was 50.30 ± 31.29 at the higher extract dose, compared to the value of 179.5 ± 45.96 achieved with the EG control. Significantly lower oxalate levels and OPN expression and increased citrate levels were observed after extract administration. In the in vitro assay, the extract diluted the formed crystals. Phytochemical analyses showed that the extract is rich in phenolic compounds that are capable of preventing stone formation. Thus, on the basis of our results, we suggest that the C. langsdorffii leaf extract has potential application in the prevention of kidney stone formation. © 2013 Rejane Barbosa de Oliveira et al.


PubMed | Apis Flora Industrial e Comercial LTDA and University of Sao Paulo
Type: | Journal: Evidence-based complementary and alternative medicine : eCAM | Year: 2015

Chamomile is a medicinal plant, which presents several biological effects, especially the anti-inflammatory effect. One of the compounds related to this effect is apigenin, a flavonoid that is mostly found in its glycosylated form, apigenin-7-glucoside (APG), in natural sources. However, the affectivity and safety of this glycoside have not been well explored for topical application. In this context, the aim of this work was to develop and validate a reversed-phase high-performance liquid chromatography (RP-HPLC-DAD) method to quantify APG in chamomile preparations. Additionally, the safety and the anti-inflammatory potential of this flavonoid were verified. The RP-HPLC-DAD method was developed and validated with linearity at 24.0-36.0g/mL range (r = 0.9994). Intra- and interday precision (RSD) were 0.27-2.66% and accuracy was 98.27-101.21%. The validated method was applied in the analysis of chamomile flower heads, glycolic extract, and Kamillen cream, supporting the method application in the quality control of chamomile preparations. Furthermore, the APG safety was assessed by MTT cytotoxicity assay and mutagenic protocols and the anti-inflammatory activity was confirmed by a diminished TNF- production showed by mice macrophages treated with APG following LPS treatment.


PubMed | São Paulo State University, Apis Flora Industrial e Comercial Ltda and University of Sao Paulo
Type: | Journal: Planta medica | Year: 2017

Artepillin C, a natural product present in the Brazilian green propolis, has several biological properties. Among these properties, the antitumor action of this product is noteworthy and makes it a promising drug candidate for the treatment of several types of cancer. This paper describes the


PubMed | University of Bristol, Academy of Sciences of the Czech Republic, Apis Flora Industrial e Comercial LTDA and University of Sao Paulo
Type: | Journal: BioMed research international | Year: 2014

Monensin A is a commercially important natural product isolated from Streptomyces cinnamonensins that is primarily employed to treat coccidiosis. Monensin A selectively complexes and transports sodium cations across lipid membranes and displays a variety of biological properties. In this study, we evaluated the Jacobsen catalyst as a cytochrome P450 biomimetic model to investigate the oxidation of monensin A. Mass spectrometry analysis of the products from these model systems revealed the formation of two products: 3-O-demethyl monensin A and 12-hydroxy monensin A, which are the same ones found in in vivo models. Monensin A and products obtained in biomimetic model were tested in a mitochondrial toxicity model assessment and an antimicrobial bioassay against Staphylococcus aureus, S. aureus methicillin-resistant, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Escherichia coli. Our results demonstrated the toxicological effects of monensin A in isolated rat liver mitochondria but not its products, showing that the metabolism of monensin A is a detoxification metabolism. In addition, the antimicrobial bioassay showed that monensin A and its products possessed activity against Gram-positive microorganisms but not for Gram-negative microorganisms. The results revealed the potential of application of this biomimetic chemical model in the synthesis of drug metabolites, providing metabolites for biological tests and other purposes.


PubMed | Apis Flora Industrial e Comercial Ltda., University of Ribeirão Preto and University of Sao Paulo
Type: | Journal: Evidence-based complementary and alternative medicine : eCAM | Year: 2015

The alternative use of natural products, like royal jelly (RJ), may be an important tool for the treatment of infections caused by antibiotic-resistant bacteria. RJ presents a large number of bioactive substances, including antimicrobial compounds. In this study, we carried out the chemical characterization of fresh and lyophilized RJ and investigated their antibacterial effects with the purpose of evaluating if the lyophilization process maintains the chemical and antibacterial properties of RJ. Furthermore, we evaluated the antibacterial efficacy of the main fatty acid found in RJ, the 10-hydroxy-2-decenoic acid (10H2DA). Chromatographic profile of the RJ samples showed similar fingerprints and the presence of 10H2DA in both samples. Furthermore, fresh and lyophilized RJ were effective against all bacteria evaluated; that is, the lyophilization process maintains the antibacterial activity of RJ and the chemical field of 10H2DA. The fatty acid 10H2DA exhibited a good antibacterial activity against Streptococcus pneumoniae. Therefore, it may be used as an alternative and complementary treatment for infections caused by antibiotic-resistant S. pneumoniae.

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